Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for +publisher:"Georgia Tech" +contributor:("Dr. Todd C. McDevitt"). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Georgia Tech

1. Charest, Joseph Leo. Topographic and chemical patterning of cell-surface interfaces to influence cellular functions.

Degree: PhD, Mechanical Engineering, 2007, Georgia Tech

This dissertation aims to further the understanding of the complex communication that occurs as cells interact with topographical and chemical patterns on a biomaterial interface. The research accomplishes this through two aims fabricating cell substrate surface topography and chemical patterns independently using non-cleanroom approaches, and analyzing higher order cellular response to surface features. The work will impact biomaterial surface modification and fabrication which will apply to biomedical implanted devices, tissue engineering scaffolds, and biological analysis devices. The first aim seeks to apply non-traditional topographical and chemical patterning methods in order to create independent topographical and chemical patterns on cell culture substrates. Experiments use the resulting patterned substrates to quantify cellular alignment to surface topography and compare the relative influence of topographical and chemical patterns on cellular response. The combined patterning methods of imprint lithography and micro-contact printing result in a high-throughput technique applicable to a variety of materials and a range of feature sizes from nanoscale through microscale, thereby enabling future analysis of cell response to surface features. The second aim evaluates the impact of topographical and chemical features on cellular differentiation. Experiments use patterned topography overlaid with a characterized chemical model layer to evaluate the effects of topography on myoblast differentiation and alignment. Chemical patterns that independently control available cell spreading area and modulate cell-cell contact are used to investigate the impact of cell-cell contact on differentiation. Advisors/Committee Members: Dr. William P. King (Committee Chair), Dr. Andres J. Garcia (Committee Member), Dr. F. Levent Degertekin (Committee Member), Dr. Hang Lu (Committee Member), Dr. Todd C. McDevitt (Committee Member).

Subjects/Keywords: Biomaterial; Cell-surface interface; Chemical pattern; Micropattern; Nanopattern; Topography; Cells; Keratinocytes; Cell adhesion; Biomedical materials; Surface chemistry

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Charest, J. L. (2007). Topographic and chemical patterning of cell-surface interfaces to influence cellular functions. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/24621

Chicago Manual of Style (16th Edition):

Charest, Joseph Leo. “Topographic and chemical patterning of cell-surface interfaces to influence cellular functions.” 2007. Doctoral Dissertation, Georgia Tech. Accessed November 24, 2020. http://hdl.handle.net/1853/24621.

MLA Handbook (7th Edition):

Charest, Joseph Leo. “Topographic and chemical patterning of cell-surface interfaces to influence cellular functions.” 2007. Web. 24 Nov 2020.

Vancouver:

Charest JL. Topographic and chemical patterning of cell-surface interfaces to influence cellular functions. [Internet] [Doctoral dissertation]. Georgia Tech; 2007. [cited 2020 Nov 24]. Available from: http://hdl.handle.net/1853/24621.

Council of Science Editors:

Charest JL. Topographic and chemical patterning of cell-surface interfaces to influence cellular functions. [Doctoral Dissertation]. Georgia Tech; 2007. Available from: http://hdl.handle.net/1853/24621

2. Ngangan, Alyssa V. Bioactive factors secreted by differentiating embryonic stem cells.

Degree: PhD, Biomedical Engineering, 2011, Georgia Tech

Current therapeutic strategies to stimulate endogenous angiogenic processes within injured tissue areas are typically based on introducing exogenous pro-angiogenic molecules or cell populations. Stem cell transplantation for angiogenic therapy aims to deliver populations of cells that secrete angiogenic factors and/or engraft in the new branching vasculature within the damaged tissue. Utilizing stem or progenitor cells has been shown to induce a rather robust angiogenic response despite minimal repopulation of the host vasculature, suggesting that stem cells may provide paracrine factors that transiently induce endogenous angiogenesis of tissues undergoing regeneration. Early differentiating embryonic stem cell (ESC) aggregates, referred to as embryoid bodies (EBs), can undergo vasculogenic differentiation, and also produce extracellular matrix and growth factors that induce proliferation, differentiation, and tissue morphogenesis. Taken together, the ESC extracellular environment may be an effective means by which to manipulate cell behavior. Thus, the objective of this project was to harness morphogens derived from ESCs undergoing differentiation and analyze their bioactive potential. To examine the expression of extracellular factors within EBs, gene expression arrays in conjunction with a variety of analytical tools were utilized to gain an understanding of the importance of extracellular factors in ESC differentiation. Furthermore, the soluble fraction of secreted factors contained within EB-conditioned media was compared to the matrix-associated factors produced by EBs, which led to the development of novel ESC-derived matrices via mechanical acellularization methods. Acellular embryonic stem cell-derived matrices demonstrated the retention of bioactive factors that impacted aspects of angiogenesis. In conclusion, extracellular factors were modulated in response to the progression of EB differentiation and can further be harnessed via acellularization techniques, in order to deliver bioactive ESC-secreted factors in a cell-free manner. Advisors/Committee Members: Dr. Todd C. McDevitt (Committee Chair), Dr. Andres J. Garcia (Committee Member), Dr. Robert E. Guldberg (Committee Member), Dr. Thomas Barker (Committee Member), Dr. Young-sup Yoon (Committee Member).

Subjects/Keywords: Angiogenesis; Extracellular matrix; Growth factors; Embryonic stem cells; Stem cells; Stem cells Research; Embryonic stem cells Research; Neovascularization

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ngangan, A. V. (2011). Bioactive factors secreted by differentiating embryonic stem cells. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/44913

Chicago Manual of Style (16th Edition):

Ngangan, Alyssa V. “Bioactive factors secreted by differentiating embryonic stem cells.” 2011. Doctoral Dissertation, Georgia Tech. Accessed November 24, 2020. http://hdl.handle.net/1853/44913.

MLA Handbook (7th Edition):

Ngangan, Alyssa V. “Bioactive factors secreted by differentiating embryonic stem cells.” 2011. Web. 24 Nov 2020.

Vancouver:

Ngangan AV. Bioactive factors secreted by differentiating embryonic stem cells. [Internet] [Doctoral dissertation]. Georgia Tech; 2011. [cited 2020 Nov 24]. Available from: http://hdl.handle.net/1853/44913.

Council of Science Editors:

Ngangan AV. Bioactive factors secreted by differentiating embryonic stem cells. [Doctoral Dissertation]. Georgia Tech; 2011. Available from: http://hdl.handle.net/1853/44913

.