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You searched for +publisher:"Georgia Tech" +contributor:("Dr. Nicholas V. Hud"). Showing records 1 – 2 of 2 total matches.

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Georgia Tech

1. Nayak, Satish Prakash. Design, Synthesis and Characterization of Multiresponsive Microgels.

Degree: PhD, Chemistry and Biochemistry, 2005, Georgia Tech

This thesis is geared towards using hydrogel nanoparticles in various biotechnological applications. The polymer that was used in making these nanoparticles was poly(N-isopropylacrylamide), which is a thermoresponsive polymer. These particles were used in making fast responsive polymer films, which can be used in optics. It was observed that the rate of deswelling increased as the concentration of the nanoparticles in the film was increased. These particles were also used in making photoresponsive materials. In this case a photoresponsive dye (malachite green) was conjugated to these nanoparticles and in presence of light of appropriate wavelength the particles undergo a phase transition. A core/shell construct was synthesized where the core was composed of degradable cross-links and the shell of composed of non-degradable cross-links. The degradable cross-linker had vicinal diols, which can be cleaved by sodium periodate. Hence after degrading the core, hollow particles were obtained. Zwitterionic particles were made by incorporating a cationic and anionic comonomer. These microgels go from a positively charged state to zwitterionic to negatively charged state on increasing the pH. One of the important potential applications for these microgels is drug delivery. Microgels were used for targeting cancer cells. Folic acid was used as the targeting ligand. The microgels were conjugated with folic acid and were able to target cells that overexpress folate receptors. In one other application core/shell microgels were made which exhibit pore-size dependent permeation of proteins. Advisors/Committee Members: Dr. L. Andrew Lyon (Committee Chair), Dr. Christopher W. Jones (Committee Member), Dr. Jiri Janata (Committee Member), Dr. Marcus Weck (Committee Member), Dr. Nicholas V. Hud (Committee Member).

Subjects/Keywords: pNIPAm; Core/Shell; Nanoparticles; Hydrogels; Polymers; Thin films; Polymers Thermal properties; Polymers Optical properties; Nanoparticles Synthesis; Colloids

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APA (6th Edition):

Nayak, S. P. (2005). Design, Synthesis and Characterization of Multiresponsive Microgels. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/6845

Chicago Manual of Style (16th Edition):

Nayak, Satish Prakash. “Design, Synthesis and Characterization of Multiresponsive Microgels.” 2005. Doctoral Dissertation, Georgia Tech. Accessed April 13, 2021. http://hdl.handle.net/1853/6845.

MLA Handbook (7th Edition):

Nayak, Satish Prakash. “Design, Synthesis and Characterization of Multiresponsive Microgels.” 2005. Web. 13 Apr 2021.

Vancouver:

Nayak SP. Design, Synthesis and Characterization of Multiresponsive Microgels. [Internet] [Doctoral dissertation]. Georgia Tech; 2005. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1853/6845.

Council of Science Editors:

Nayak SP. Design, Synthesis and Characterization of Multiresponsive Microgels. [Doctoral Dissertation]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/6845


Georgia Tech

2. Manning, Linda. The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds.

Degree: PhD, Chemistry and Biochemistry, 2005, Georgia Tech

Nitrochemicals are currently widely used as solvents, drugs, biocides, fuels and explosives and are consequently widely distributed in the environment. The reductive nitrite elimination from explosive compounds is catalyzed by two FMN-dependent, xenobiotic reductases (XenA or XenB). These genes for these regiospecific enzymes were cloned from Pseudomonas putida and P. fluorescens I-C respectively and isolated from the soil of a contaminated World War II munitions manufacturing plant. These enzymes enable the microbes to fulfill their nitrogen requirements from nitroglycerin by catalyzing the regiospecific, NADPH dependent, reductive denitration of nitroglycerin with differing selectivities. The two enzymes also transform a number of additional nitrocompounds in vitro, e.g. TNT and metronidazole, a leading drug in the treatment of Helicobacter pylori, a causative agent of human ulcers. Single crystals were obtained for XenA and XenB and complete X-ray diffraction datasets have been collected and analyzed to better understand these characteristics. The 1.6 Å resolution structure of XenA reveals a dimer of β/α)₈-TIM barrels, but the 2.3 Å resolution structure for XenB is a monomer. The (β/α)₈-TIM barrel protein fold is the most common fold in the PDB. However, the XenA structure exhibits a unique, C-terminal domain-swapped topology. Thus a portion of each active site is comprised of residues from the neighboring monomer. To probe the reaction cycle, crystal structures of ligand complexes and the reduced enzyme have been refined. For example, our structure of the XenA-metronidazole complex shows that ligands bind parallel to the FMN si-face. Our 1.5 Å resolution structure for reduced XenA reveals an FMN isoalloxazine ring with an angle of ~165° along the N5-N10 axis. We have also generated models of the reduced enzyme-nitroglycerin complexes by molecular dynamics. The results with both XenA and XenB reveal differences in enzyme-ligand hydrogen bonding. These differences correlate remarkably well with the regiospecific differences observed for nitrite elimination from nitroglycerin and reduction of TNT by the two enzymes. Advisors/Committee Members: Allen M. Orville (Committee Chair), Dr. Dale E. Edmondson (Committee Member), Dr. Frank E. Löffler (Committee Member), Dr. Loren D. Williams (Committee Member), Dr. Nicholas V. Hud (Committee Member).

Subjects/Keywords: Nitrocompounds; Flavoprotein; X-ray Crystallography; Xenobiotics; Reductones; Flavoproteins; X-ray crystallography; Nitrogen compounds; Crystals Structure

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Manning, L. (2005). The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/36528

Chicago Manual of Style (16th Edition):

Manning, Linda. “The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds.” 2005. Doctoral Dissertation, Georgia Tech. Accessed April 13, 2021. http://hdl.handle.net/1853/36528.

MLA Handbook (7th Edition):

Manning, Linda. “The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds.” 2005. Web. 13 Apr 2021.

Vancouver:

Manning L. The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds. [Internet] [Doctoral dissertation]. Georgia Tech; 2005. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1853/36528.

Council of Science Editors:

Manning L. The crystal structures of xenobiotic reductase A and B from pseudomonas putida II-B and pseudomonas fluorescens I-C: structural insight into regiospecific reactions with nitrocompounds. [Doctoral Dissertation]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/36528

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