Gross, Jeffrey David.
Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes.
Degree: PhD, Biomedical Engineering, 2007, Georgia Tech
Design and characterization of tissue engineered substitutes rely on robust monitoring techniques that provide information regarding viability and function when exposed to various environmental conditions. In vitro studies permit the direct monitoring of cellular and construct changes because these substitutes remain accessible. However, upon in vivo implantation, changes in cell viability and function are often detected using indirect or invasive methods that make assessing temporal changes challenging. . Thus, the development of non-invasive monitoring modalities may facilitate improved tissue substitute design and, ultimately, clinical outcome.
The overall objective of this thesis was to establish a method to monitor and track cells and the cellular environment within a tissue engineered substitute in vitro and in vivo. This was accomplished via 31P NMR spectroscopy and through the incorporation of perfluorocarbon (PFC) emulsions for the monitoring of DO concentration by 19F NMR spectroscopy. The first aim of this thesis was to develop a method that tracked the state of cells and of the cellular environment within alginate constructs during perfusion studies in which the perfusing medium DO concentrations were changed over time or cells were exposed to a cytotoxic antibiotic. Due to challenges in acquiring DO concentration gradient information within beads, a second aim was to develop a mathematical model that would calculate gradients from experimentally acquired volume averaged DO concentrations; thus, significantly enhancing the robustness of tracking the alginate beads. Lastly, since the PFC emulsions used in the study may affect cell viability and function, a third aim was to characterize, experimentally and via modeling, the effect of several PFC emulsion concentrations on the encapsulated and946;TC-tet cells.
Advisors/Committee Members: Sambanis, Athanassios (Committee Chair), Chaikof, Elliot (Committee Member), Constantinidis, Ioannis (Committee Member), Edmondson, Dale (Committee Member), Long, Jr., Robert (Committee Member).
Subjects/Keywords: 19F NMR; Perfluorocarbon; Pancreatic substitute; Oxygen
to Zotero / EndNote / Reference
APA (6th Edition):
Gross, J. D. (2007). Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14499
Chicago Manual of Style (16th Edition):
Gross, Jeffrey David. “Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes.” 2007. Doctoral Dissertation, Georgia Tech. Accessed March 07, 2021.
MLA Handbook (7th Edition):
Gross, Jeffrey David. “Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes.” 2007. Web. 07 Mar 2021.
Gross JD. Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes. [Internet] [Doctoral dissertation]. Georgia Tech; 2007. [cited 2021 Mar 07].
Available from: http://hdl.handle.net/1853/14499.
Council of Science Editors:
Gross JD. Non-invasive Monitoring of Oxygen Concentrations and Metabolic Function in Pancreatic Substitutes. [Doctoral Dissertation]. Georgia Tech; 2007. Available from: http://hdl.handle.net/1853/14499