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You searched for +publisher:"Freie Universität Berlin" +contributor:("[email protected]"). One record found.

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Freie Universität Berlin

1. Kempiners, Nina. Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice.

Degree: 2013, Freie Universität Berlin

Introduction: Manifold epidemiological studies have shown that low birth weight and fetal deprivation are associated with disease in later life. This correlation has been shown especially for cardiovascular disease, arteriosclerosis, diabetes mellitus, hyperlipidaemia and obesity. On the experimental side studies with homocygous eNOS-knockout-mice could support these data. Unfortunately the existing experimental designs do not distinguish between fetal, maternal and paternal genotype. Moreover, the programming of the immune system of eNOS-knockout-mice has hardly been investigated yet. This study aims to provide data for a better understandig of the basic principles of fetal programming. Methods: In this study heterocygous eNOS-knockout-mice were crossed with wildtype mice. Only the wildtype offspring was included and divided into three groups referring to the cross. Group 1: Father WT x Mother WT, Group 2: Father WT x Mother eNOS+/-, Group 3: Father eNOS+/- x Mother WT. The study design allows the correlation of the fetal phenotype and maternal or paternal genotype. Biometric data was collected over 140 days. After 140 days the animals were killed and the organs were processed. For further analysis of the immune system the spleen was investigated with histological and immunohistochemical methods. Results: Biometric data and growth patterns showed significant and partially sex-dependent differences between the three groups. The histological and immunohistochemical analysis of the spleen showed no relevant differences concerning the investigated parameters. Discussion: The differences concerning the biometric data can be seen as a result of fetal programming. This can be explained by the modified uterine environment of the heterocygous eNOS-knockout-mouse. Moreover, paternal epigenetic mechanisms seem to play an important role as well. The programming effect seems to be sex-dependent because female animals are not affected in the same way as male animals. The investigation of the spleen did not show any programming effect referring to the immune system in this study constellation. These results can only partially stand for the immune system as a whole because only the cellular components of one organ were studied. Further research with immune function parameters and a longer span of life of the animals is needed. Advisors/Committee Members: [email protected] (contact), w (gender), Prof. Dr. B. Hocher (firstReferee), Prof. Dr. J.-P. Stasch, Prof. Dr. med. M. Schwab (furtherReferee).

Subjects/Keywords: fetal programming; eNOS Knockout; spleen; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kempiners, N. (2013). Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/12218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Kempiners, Nina. “Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice.” 2013. Thesis, Freie Universität Berlin. Accessed December 11, 2019. https://refubium.fu-berlin.de/handle/fub188/12218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Kempiners, Nina. “Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice.” 2013. Web. 11 Dec 2019.

Vancouver:

Kempiners N. Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2019 Dec 11]. Available from: https://refubium.fu-berlin.de/handle/fub188/12218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kempiners N. Fetal programming of wildtype offspring of heterocygous eNOS-knockout-mice. [Thesis]. Freie Universität Berlin; 2013. Available from: https://refubium.fu-berlin.de/handle/fub188/12218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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