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You searched for +publisher:"Freie Universität Berlin" +contributor:("Univ.-Prof. Dr. T. Skutella"). One record found.

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Freie Universität Berlin

1. Krämer, Nadine. Developmental molecular factors of the dentate gyrus in mice.

Degree: 2008, Freie Universität Berlin

The dentate gyrus provides a good model for diverse areas of research concerning the cortical development, especially neurogenesis, neuronal migration, regionalization, axonal guidance and neuronal plasticity. Furthermore, the dentate gyrus has the special ability to produce functional neurons out of neuronal precursors the whole life long. Therefore it is an interesting object of research for potential neuronal processes of regeneration in the cortex. The aim of this study was to identify molecular factors of dentate gyrus development and to analyse their functions. The subject of the experiments was a comparison of expression patterns from Emx2-/- mutant versus wildtype mouse, because the transcription factor Emx2 plays a key role in hippocampal development. Thus, mice deficient in this factor show very specific developmental alterations in the hippocampus, which leads to the absence of a morphologically visible dentate gyrus in these Emx2-/- mutants. First some known genes, whose functions had already been described in literature extensively, were analysed during development by in situ hybridisation. Thereby investigating the different functional circuits like proliferation, differentiation and migration as well as the structural alterations of the dentate gyrus and the adjacent hippocampal fissure and marginal zone were of particular interest. Furthermore some candidate genes, for which a putative relation to Emx2 had not been described previously, were identified by screening microarrays. The results show that, in the Emx2-/- mutant dentate gyrus, the proneural cascade of bHLH genes is present, but abnormally confined to the germinal layer, the neuronal and glial migrating precursors are nearly absent, the glial scaffold is defective, the few produced astrocytes and granule cells are not able to migrate to their proper destination and in addition the granule cells are deficiently differentiated. The glial scaffolding is also lacking in the Emx2-/- mutant developing hippocampal fissure. The inner marginal zone (IMZ) seems to be developed properly, in contrast the outer marginal zone (OMZ) as well as the dentate marginal zone (DMZ) is atrophic. Most of the Reln-expressing cells that should occupy the OMZ and DMZ are missing, except a specific subpopulation, characterized by using additional markers, probably born in the hem and for which Emx2 is not essentially required. The microarray analysis reveals a group of genes whose expression is reduced in the Emx2-/- hippocampus. For most of them a putative relation to Emx2 pathways had not been recognized previously. Some candidates are development genes or are involved in functional maturation and show expression in the hippocampal plate and/or developing dentate gyrus. A second group of candidates labels neuronal, glial or vascular structures in the OMZ and likely represent markers for cell populations specifically absent in the mutant. The lack of these genes points at different pathways and processes altered in the Emx2-/-mutant, particularly the Notch and… Advisors/Committee Members: n (gender), Univ.-Prof. Dr. H. Martens (firstReferee), Univ.-Prof. Dr. T. Skutella (furtherReferee), Univ.-Prof. Dr. H. Fink (furtherReferee).

Subjects/Keywords: Mice, hippocampus, embryonic development, neurons, migration, genes; Genes, Homeobox (MeSH); gene expression; Gene Expression Regulation, Developmental (MeSH); transcription factors, neuroglia, polymerase chain reaction, DNA microarrays, in-situ hybridization; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA (6th Edition):

Krämer, N. (2008). Developmental molecular factors of the dentate gyrus in mice. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Thesis, Freie Universität Berlin. Accessed January 15, 2021. http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Web. 15 Jan 2021.

Vancouver:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 15]. Available from: http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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