+publisher:"Freie Universität Berlin" +contributor:("Univ.-Prof. Dr. H. Fink")

You searched for +publisher:"Freie Universität Berlin" +contributor:("Univ.-Prof. Dr. H. Fink"). Showing records 1 – 12 of 12 total matches.

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Freie Universität Berlin

1. Walter, Alice. Assessment of postnatal persistence of prenatally induced effects on the skeletal system of rats after treatment with the classic teratogen 5-fluoro-2’-deoxyuridine (FUDR).

Degree: 2015, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-4370

► In developmental toxicology adverse effects of chemical substances on fetuses during in utero development are investigated. One important manifestation of such adverse effects are changes… (more)

▼ In developmental toxicology adverse effects of chemical substances on fetuses during in utero development are investigated. One important manifestation of such adverse effects are changes in skeletal development during organogenesis, which are generally called anomalies. During risk assessment of anomaly- inducing substances the classification of the findings into variation or malformation is crucial for their categorization as potential reproductive toxicant. While a variation is characterized by a delay in development a malformation represents a permanent irreversible impairment which can affect postnatal survival or health. However, postnatal permanence cannot be assessed in routine studies on developmental toxicity as sacrifice and evaluation of fetuses are carried out one day before their calculated delivery. Furthermore, in part of the skeletal system, especially in the cervical column, development is completed postnatally. Few publications exist that address the topic of postnatal effects of substances. For the past three decades experts have emphasized the need for studies on postnatal consequences of fetal anomalies in order to reduce uncertainties regarding their classification. The aim of the present investigation is to contribute to clarification of the postnatal fate of skeletal anomalies in the vertebral column. For that purpose already processed skeletons of rat fetuses and offspring from earlier experiments with the substance 5-fluoro-20-deoxyuridine (FUDR) were assessed. FUDR is known to induce teratological effects and is frequently used as model substance in teratological studies. Dams in the treatment groups received a single subcutaneous dose of 35, 40, 45, 50, 55, 65 and 75 mg FUDR/kg body weight (bw) on day 11 of gestation. The control group was treated with the vehicle aqua dest. at a volume of 10 ml/kg bw. Offspring were sacrificed on gestational day 21 and on postnatal days 7 and 21. Subsequently animals were subject to the double-staining procedure with alcian blue and alizarin red. Evaluation of the skeletons was carried out with 4-fold magnification under a binocular microscope. All findings were assigned according to the updated internationally harmonized terminology. Based on the results obtained postnatally recommendation for classification of several anomalies were elaborated in the present study. We recommend the classification of the anomalies vertebra centrum unossified, vertebra centrum asymmetric ossification, vertebra centrum bipartite ossification, vertebra centrum hemicentric (with the exception of cervical vertebrae) as well as vertebra centrum misshapen (with the exception of lumbar vertebrae) as variations. The anomaly vertebra centrum dumbbell-shaped should be classified as malformation based on ist postnatal persistence. The same applies to the anomaly lumbar centrum supernumerary sinister/dexter/sinister+dexter which should also be classified as malformation. The present study contributes to the reduction in uncertainty when it comes to the classification of skeletal… Advisors/Committee Members: w (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (firstReferee), Prof. Dr. I. Chahoud (furtherReferee), PD Dr. P. Hauff (furtherReferee).

Subjects/Keywords: rats; postnatal period; toxicology; deoxyuridine; malformations; teratogens; spine; microscopy; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Walter, A. (2015). Assessment of postnatal persistence of prenatally induced effects on the skeletal system of rats after treatment with the classic teratogen 5-fluoro-2’-deoxyuridine (FUDR). (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-4370

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Walter, Alice. “Assessment of postnatal persistence of prenatally induced effects on the skeletal system of rats after treatment with the classic teratogen 5-fluoro-2’-deoxyuridine (FUDR).” 2015. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-4370.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Walter A. Assessment of postnatal persistence of prenatally induced effects on the skeletal system of rats after treatment with the classic teratogen 5-fluoro-2’-deoxyuridine (FUDR). [Internet] [Thesis]. Freie Universität Berlin; 2015. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-4370.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Walter A. Assessment of postnatal persistence of prenatally induced effects on the skeletal system of rats after treatment with the classic teratogen 5-fluoro-2’-deoxyuridine (FUDR). [Thesis]. Freie Universität Berlin; 2015. Available from: http://dx.doi.org/10.17169/refubium-4370

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

2. Flechsig, geb. Lerche, Susanne. Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs.

Degree: 2011, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-10008

► In this doctoral thesis a tumor surrogate angiogenesis model for in vivo quantitation of antiangiogenic therapeutic effects was established and characterized by using contrast enhanced… (more)

▼ In this doctoral thesis a tumor surrogate angiogenesis model for in vivo quantitation of antiangiogenic therapeutic effects was established and characterized by using contrast enhanced micro-ultrasound. For this purpose, initially three animal experiments were conducted where angiogenesis of the surrogate model was investigated both with microultrasound and histology. Finally, the surrogate model was used in a therapeutic setting for the first time. The first experiment was conducted by using immunodeficient mice. Proangiogenic growth factor containing alginate beads as surrogate for tumor xenografts were injected subcutaneously. Due to in vivo release of proangiogenic growth factors angiogenesis was induced into the alginate bead depots. Growing blood vessels could be detected with contrast enhanced micro- ultrasound. A significant difference in vascularization of growth factor containing and empty alginates could be quantified. Results were confirmed by a positive correlation of ultrasound contrast signal intensity and histological blood vessel density determined by using stereological tools. Aim of the second experiment was to observe the angiogenic process within individual alginate bead depots by micro-ultrasound. Similar to the first experiment growth factor containing or empty depots were injected subcutaneously in immunodeficient mice. The progressive vascularization of individual alginates was observed by micro-ultrasound. Early after study start a significant difference could be detected between growth factor containing and empty depots, which could be strongly confirmed by histology. The third study was carried out based on the results of study one and two. The progressing vascularization of growth factor containing and empty alginate bead depots was determined with contrast enhanced micro-ultrasound. Immunocompetent mice were used. Results showed no impact of immune status on ultrasound measurements of alginate bead depot angiogenesis. Finally, the established tumor surrogate angiogenesis model was used in a therapeutic experiment for the first time. Growth factor containing alginate bead depots were injected subcutaneously to immunocompetent mice. Mice were treated daily with Bevacizumab, an antiangiogenic anti-VEGF monoclonal antibody or with Regorafenib, an antiangiogenic oral multityrosine kinase inhibitor developed by Bayer Schering Pharma AG (BAY73-4506). Control groups were made up and treated with vehicles of Bevacizumab or Regorafenib. Micro-ultrasound measurements of individual alginate bead depot vascularization were conducted once weekly. Inhibition of angiogenesis due to treatment could be quantified in vivo early after study start. Significant differences between vehicle and drug treated groups were observed early. Correlation of micro-ultrasound and histological data showed a clear correlation of blood vessel profile density and contrast signal intensity. These results illustrate, that contrast enhanced micro-ultrasound is a fast, efficient and minimally invasively method for the… Advisors/Committee Members: w (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Fr. Univ.-Prof. Dr. H. Fink (firstReferee), Hr. PD Dr. P. Hauff (furtherReferee), Prof.%20Dr.%20F.%20Kiessling%22%29&pagesize-30">Hr. Univ.-Prof. Dr. F. Kiessling (furtherReferee).

Subjects/Keywords: mice; angiogenesis; Cancer; xenografts; ultrasound; diagnostic techniques; animal models; alginates; growth factors; histology;

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Flechsig, geb. Lerche, S. (2011). Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-10008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Flechsig, geb. Lerche, Susanne. “Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs.” 2011. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-10008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Flechsig, geb. Lerche, Susanne. “Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs.” 2011. Web. 19 Jan 2021.

Vancouver:

Flechsig, geb. Lerche S. Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-10008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Flechsig, geb. Lerche S. Establishment of an in vivo tumor surrogate angiogenesis model for testings of new antiangiogenic drugs. [Thesis]. Freie Universität Berlin; 2011. Available from: http://dx.doi.org/10.17169/refubium-10008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

3. Krämer, Nadine. Developmental molecular factors of the dentate gyrus in mice.

Degree: 2008, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-8178

► The dentate gyrus provides a good model for diverse areas of research concerning the cortical development, especially neurogenesis, neuronal migration, regionalization, axonal guidance and neuronal… (more)

▼ The dentate gyrus provides a good model for diverse areas of research concerning the cortical development, especially neurogenesis, neuronal migration, regionalization, axonal guidance and neuronal plasticity. Furthermore, the dentate gyrus has the special ability to produce functional neurons out of neuronal precursors the whole life long. Therefore it is an interesting object of research for potential neuronal processes of regeneration in the cortex. The aim of this study was to identify molecular factors of dentate gyrus development and to analyse their functions. The subject of the experiments was a comparison of expression patterns from Emx2-/- mutant versus wildtype mouse, because the transcription factor Emx2 plays a key role in hippocampal development. Thus, mice deficient in this factor show very specific developmental alterations in the hippocampus, which leads to the absence of a morphologically visible dentate gyrus in these Emx2-/- mutants. First some known genes, whose functions had already been described in literature extensively, were analysed during development by in situ hybridisation. Thereby investigating the different functional circuits like proliferation, differentiation and migration as well as the structural alterations of the dentate gyrus and the adjacent hippocampal fissure and marginal zone were of particular interest. Furthermore some candidate genes, for which a putative relation to Emx2 had not been described previously, were identified by screening microarrays. The results show that, in the Emx2-/- mutant dentate gyrus, the proneural cascade of bHLH genes is present, but abnormally confined to the germinal layer, the neuronal and glial migrating precursors are nearly absent, the glial scaffold is defective, the few produced astrocytes and granule cells are not able to migrate to their proper destination and in addition the granule cells are deficiently differentiated. The glial scaffolding is also lacking in the Emx2-/- mutant developing hippocampal fissure. The inner marginal zone (IMZ) seems to be developed properly, in contrast the outer marginal zone (OMZ) as well as the dentate marginal zone (DMZ) is atrophic. Most of the Reln-expressing cells that should occupy the OMZ and DMZ are missing, except a specific subpopulation, characterized by using additional markers, probably born in the hem and for which Emx2 is not essentially required. The microarray analysis reveals a group of genes whose expression is reduced in the Emx2-/- hippocampus. For most of them a putative relation to Emx2 pathways had not been recognized previously. Some candidates are development genes or are involved in functional maturation and show expression in the hippocampal plate and/or developing dentate gyrus. A second group of candidates labels neuronal, glial or vascular structures in the OMZ and likely represent markers for cell populations specifically absent in the mutant. The lack of these genes points at different pathways and processes altered in the Emx2-/-mutant, particularly the Notch and… Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Martens%22%29&pagesize-30">Univ.-Prof. Dr. H. Martens (firstReferee), Prof.%20Dr.%20T.%20Skutella%22%29&pagesize-30">Univ.-Prof. Dr. T. Skutella (furtherReferee), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (furtherReferee).

Subjects/Keywords: Mice, hippocampus, embryonic development, neurons, migration, genes; Genes, Homeobox (MeSH); gene expression; Gene Expression Regulation, Developmental (MeSH); transcription factors, neuroglia, polymerase chain reaction, DNA microarrays, in-situ hybridization; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krämer, N. (2008). Developmental molecular factors of the dentate gyrus in mice. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Web. 19 Jan 2021.

Vancouver:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

4. Ehrentraut, Claudia. Investigation of the DNA adduct forming potential from selected pharmaceuticals.

Degree: 2009, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-9483

► DNA adducts are of interest both because of their possible direct involvement in mutagenic and carcinogenic processes and as indicators of exposure to mutagens. For… (more)

▼ DNA adducts are of interest both because of their possible direct involvement in mutagenic and carcinogenic processes and as indicators of exposure to mutagens. For several environmentally or occupationally relevant compounds, i.e. benzo(a)pyrene and several aromatic amines, the different steps in the process of carcinogenesis initiated by DNA adduct formation are well established. However, pharmaceuticals are less well investigated. To broaden the knowledge about the adduct forming potential of pharmaceuticals we selected in this thesis pharmaceuticals which should be investigated with the 32P-postlabeling assay in rat liver slices. We selected drugs which had been on the German market for at least 30 years and which had a molecular weight in the range of 100 to 600 g/mol. These criteria were chosen to ensure that the latency period of most cancers was surpassed and that because of their volume the DNA-adducts would be detectable in the 32P postlabeling assay. By means of the IARC-Classification, the DEREK-program and an epidemiologic literature screen we further divided the received 734 drugs and selected them representatively. After incubation of the pharmaceuticals with the rat liver slices in a submerged incubation system the DNA was isolated. The originated DNA-adducts were enriched, labeled with 32P and visualized with a thin layer chromatography-process. In this thesis we showed that the chosen test system which had been optimized to investigate DNA reactivity of steroidal compounds is able to detect DNA-adducts from various classes of pharmaceuticals. However, several drugs which were expected to cause adducts were surprisingly negative, namely alkylating antineoplastics like chlorambucil, cyclophosphamide and melphalan. Most probably the structural changes of the DNA bases caused by alkylation were too small to be chromatographically detectable. In addition we could show that the adduct-level and the specific adduct-pattern depends on several factors, for example age, gender, tissue and species, and the examination will be hindered due to I-compounds. However, the DNA-adduct analysis in tissue slices enabled an examination of different drugs and concentrations in one liver in only one in vitro experiment. Due to the fact that different tissues (in this thesis: liver and kidney) could be chosen, the efficiency and the significance of this method was broadened. Even the use of only small amount from substance is another benefit of this procedure. Although this procedure is very complex and time-consuming, the analysis could be chosen to clear up risk assessments from inconsistent combination of positive and negative results in carcinogenic and mutagenic experiments. Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Frau Univ.-Prof. Dr. H. Fink (firstReferee), Herr Prof. Dr. W. Kuhnz (furtherReferee), PD Dr. Michael Veit (furtherReferee).

Subjects/Keywords: dna; binding; dna adducts (MeSH); rats; liver; kidneys; culture techniques; phosphorus radioisotopes (MeSH); thin layer chromatography; steroids; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ehrentraut, C. (2009). Investigation of the DNA adduct forming potential from selected pharmaceuticals. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-9483

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ehrentraut, Claudia. “Investigation of the DNA adduct forming potential from selected pharmaceuticals.” 2009. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-9483.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ehrentraut, Claudia. “Investigation of the DNA adduct forming potential from selected pharmaceuticals.” 2009. Web. 19 Jan 2021.

Vancouver:

Ehrentraut C. Investigation of the DNA adduct forming potential from selected pharmaceuticals. [Internet] [Thesis]. Freie Universität Berlin; 2009. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-9483.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ehrentraut C. Investigation of the DNA adduct forming potential from selected pharmaceuticals. [Thesis]. Freie Universität Berlin; 2009. Available from: http://dx.doi.org/10.17169/refubium-9483

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

5. Holzapfel, Judith Maria. Use of propofol as a short-term anesthetic in domestic waterfowl.

Degree: 2009, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/12378

► The aim of this study was to verify the suitability of propofol for minor diagnostic or thera-peutic procedures in mute swans (Cygnus olor) and mallard… (more)

▼ The aim of this study was to verify the suitability of propofol for minor diagnostic or thera-peutic procedures in mute swans (Cygnus olor) and mallard ducks (Anas platyrhynchos). In the present study 30 free-living mute swans and seven mallard ducks were given propofol as part of minor diagnostic and/or therapeutic procedures. The birds were randomly divided into two groups. Induction of anesthesia was accomplished by administering 8 mg/kg of propofol. The birds of the first group were given additional boluses (2-4 mg/kg of propofol every one to seven minutes) as required to attain a light plain of anesthesia. In the other birds the induction was followed by a constant rate infusion (CRI) of propofol (0.85 mg/kg/min). After receiving the loading dose no bird showed apnea and all birds could be easily intubated. Periodic bolus injection requires constant assessment and adjustment of anesthetic depth. Birds woke up unexpectedly during anesthesia with bolus administration. Therefore constant rate infusion is superior to and much more practical than bolus administration. Body temperature, heart rate, respiratory rate, saturation with oxygen and reflexes were comparable in both groups. In mute swans the loss in body temperature was 0.75°C (bolus) and 0.85°C (CRI) within 35 minutes, and in mallard ducks 0.8°C (bolus) and 0.9°C (CRI) within 35 minutes. Heart rate decreased from 164 to 129 bpm (bolus) and from 221 to 191 bpm (CRI) in mute swans, and from 310 to 210 bpm (bolus) and from 367 to 337 bpm (CRI) in mallard ducks. Respiratory rate varied in mute swans during anesthesia with boluses and decreased during CRI from eight to 12 breaths per minute. In mallard ducks the respiratory rate did not change significantly over time (bolus) and decreased during CRI from 37 to 34 breaths per minute. Saturation with oxygen varied in all birds, increased in mute swans from 69 % to 81 % (bolus) and in mallard ducks from 61 % to 88 % (bolus) and from 79 % to 82 % (CRI). There was no increase of saturation with oxygen in mute swans during CRI. During CRI all mute swans and during boluses and CRI all mallard ducks had corneal reflex. During boluses corneal reflex was temporarily delayed in three mute swans and lost in one. The swallowing reflex was lost in most birds and delayed in some birds. Most birds always displayed pedal reflex. All birds recovered from anesthesia without any long-term effects. At the latest 40 minutes after the end of anesthesia all birds had fully recovered. 60 % (12/30) of the mute swans and 71 % (5/7) of the mallard ducks examined showed transient signs of central nervous system excitement during recovery, which subsided after a few minutes. Besides respiratory depress-sion and excitatory phenomena during recovery, no other side effects of propofol occurred during this study. Propofol is a good anesthetic agent for minor procedures in mute swans and mallard ducks – especially given as constant rate infusion. Advisors/Committee Members: n (gender), Prof.%20Dr.%20L.%20Brunnberg%22%29&pagesize-30">Univ.-Prof. Dr. L. Brunnberg (firstReferee), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (furtherReferee), Prof.%20Dr.%20H.%20T%C3%83%C2%B6nhardt%22%29&pagesize-30">Univ.-Prof. Dr. H. Tönhardt (furtherReferee).

Subjects/Keywords: anaesthesia; propofol; injectable anaesthetics; infusion; boluses; swans; ducks; anatidae; water fowl; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Holzapfel, J. M. (2009). Use of propofol as a short-term anesthetic in domestic waterfowl. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/12378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Holzapfel, Judith Maria. “Use of propofol as a short-term anesthetic in domestic waterfowl.” 2009. Thesis, Freie Universität Berlin. Accessed January 19, 2021. https://refubium.fu-berlin.de/handle/fub188/12378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Holzapfel, Judith Maria. “Use of propofol as a short-term anesthetic in domestic waterfowl.” 2009. Web. 19 Jan 2021.

Vancouver:

Holzapfel JM. Use of propofol as a short-term anesthetic in domestic waterfowl. [Internet] [Thesis]. Freie Universität Berlin; 2009. [cited 2021 Jan 19]. Available from: https://refubium.fu-berlin.de/handle/fub188/12378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Holzapfel JM. Use of propofol as a short-term anesthetic in domestic waterfowl. [Thesis]. Freie Universität Berlin; 2009. Available from: https://refubium.fu-berlin.de/handle/fub188/12378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

6. Tellmann, Arn. Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs.

Degree: 2008, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/13035

► To be able to use the Göttingen minipig as an experimental animal model for endocrinological and pharmacological objectives, it must be possible to evaluate the… (more)

▼ To be able to use the Göttingen minipig as an experimental animal model for endocrinological and pharmacological objectives, it must be possible to evaluate the pharmacological effect of a substance on the ovarian cycle of the animals. In a preliminary study, methods were tested for their practicability and significance to characterize the cycle by using six female minipigs. Suitable proved to be the natural toleration reflex, the redness/swelling of the vulva, the evaluation of the vaginal mucus, the ultrasound examination of the ovaries as well as the determination of the plasma concentrations of luteinizing hormone, follicle stimulating hormone, 17-ß-estradiol and progesterone. In a main study, 12 female animals (3 treatment groups, 1 control group) were treated intramuscularly for 24 days with levonorgestrel (LNG), a synthetic gestagen. The aim was to inhibit the ovulation of the animals and to determine the threshold dose for the inhibition of ovulation. The lowest pharmacologically effective dose for the inhibition of ovulation was determined to be 0.02 mg/kg/day LNG. In the 0.002 mg/kg/day dose group, no clear estrus symptoms were evident on the outer genital. However, ovulation was confirmed by an ultrasound examination as well as the hormone concentrations at the end of the study. In the 0.02 mg/kg/day and 0.1 mg/kg/day dose groups, both behavior and all other measured parameters showed a clear interruption of the sexual cycle, and no ovulation occurred. Animals in both the mid- and high dose groups showed substance related effects, not only on the ovary but also on the vagina, uterus, and the organ weights. These results correlated with known effects observed in other species, e.g. the rat. Overall, the Göttinger minipig proved to be a very suitable animal model to test pharmacological hormone effects on the sexual cycle of the minipig. This was also applicable to the choice of methods, which offered an additional possibility to evaluate the gestagenic effects of hormones and other substances in toxicology studies. Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (firstReferee), Prof. Dr. C. Hegele-Hartung (furtherReferee), H.%20Lahrmann%22%29&pagesize-30">Prof. Dr. K.-H. Lahrmann (furtherReferee).

Subjects/Keywords: Pigs; miniature pigs; laboratory animals; progesterone; steroid hormones; synthetic progestogens; ouvlation; animal behaviour; ultrasonography; ovary; endometrium; histology; LH; FSH; estradiol; quantitative analysis; pharmacokinetics; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tellmann, A. (2008). Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tellmann, Arn. “Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs.” 2008. Thesis, Freie Universität Berlin. Accessed January 19, 2021. https://refubium.fu-berlin.de/handle/fub188/13035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tellmann, Arn. “Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs.” 2008. Web. 19 Jan 2021.

Vancouver:

Tellmann A. Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 19]. Available from: https://refubium.fu-berlin.de/handle/fub188/13035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tellmann A. Effects of levonorgestrel on the ovarian cycle of Göttingen minipigs. [Thesis]. Freie Universität Berlin; 2008. Available from: https://refubium.fu-berlin.de/handle/fub188/13035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

7. Meier-Ince, Beatrix von. In vitro Optimierung der Mikrodialyse für pharmakokinetische.

Degree: 2008, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/13808

► Einleitung Die Mikrodialyse wurde ursprünglich in der Neuropharmakologie, zur Messung von Neurotransmitter entwickelt (Ungerstedt, Herrera-Marschitz et al., 1982). Seit 1990 wurde die Mikrodialyse auch für… (more)

▼ Einleitung Die Mikrodialyse wurde ursprünglich in der Neuropharmakologie, zur Messung von Neurotransmitter entwickelt (Ungerstedt, Herrera-Marschitz et al., 1982). Seit 1990 wurde die Mikrodialyse auch für pharmakokinetische Untersuchungen exogener Substanzen eingesetzt (Ståhle, Segersvärd et al., 1990). Problematisch sind hierbei lipophile Substanzen, die an der Sondenmembran und am Schlauch haften. Die Mikrodialyse dieser Substanzen ist mit niedrigen Wiederfindungen (Carneheim and Ståhle, 1991) und langen Equilibrationszeiten verbunden. Die genaue Equilibrationszeit wird aber selten berichtet (Tao and Hjorth, 1992), dabei ist eine kurze Equilibrationszeit wichtig, wenn schnelle Konzentrationsänderungen gemessen werden sollen. Diese Dissertation hat daher zum Ziel ein Testverfahren zu etablieren, welches die Substanzbindung am eingesetzten Material, und die Reaktionszeit des Mikrodialyseaufbaus auf Konzentrationsänderungen quantitativ erfassen kann, damit Schlauch- und Sondenmaterialien identifiziert werden können, die für pharmakokinetische Anwendungen geeignet sind. Materialien Ein Material, dass für pharmakokinetische Studien eingesetzt werden soll, muss schnell auf Konzentrationsänderungen reagieren, und muss eine genügende Wiederfindung haben, damit die Substanzkonzentrationen in den gesammelten Proben ausreichen, um mit dem vorhandenen analytischen Verfahren quantifiziert werden zu können. Die Materialien sollten daher keine Bindung mit der Testsubstanz eingehen. Um dies zu belegen, werden Schläuche und Sonden getrennt getestet, denn nur wenn der Sammelschlauch (= Outlet) die Substanz nicht bindet, kann die Adhäsion am Sondenmaterial gemessen werden. Für die Dissertation waren 5 für die Mikrodialyse geeignete Schlauchmaterialien, und 11 unterschiedliche Hirnsonden im Handel erhältlich (Tabelle 1 und Tabelle 2). Fettgedruckt sind die Eigenschaften der Sonden, die in Bezug auf Bindungskapazität und Reaktionszeit verglichen werden: unterschiedliche Membranmaterialien und gleiches Membranmaterial aber mit unterschiedlicher Porengröße, Membrangröße, Membrandicke oder Outletmaterial. Die für die Mikrodialyse relevanten Eigenschaften der beiden Testsubstanzen sind in Tabelle 3 aufgelistet. ZK 975 ist eine lipophile Substanz, die in Vorversuchen als 'klebrig' erkannt worden war, ZK 894 ist eine hydrophile Substanz, die sich vorher als unproblematisch erwiesen hatte. Methode Für den Schlauchtest wurde jeder Schlauch (N = 4 pro Material) über Nacht mit Ringerlösung vorgespült, was zu reproduzierbareren Ergebnisse führte als mit Schläuchen, die direkt von der Packung für den Test eingesetzt wurden. Der Test bestand aus eine Expositionsphase von zirka einer Stunde, wobei der Schlauch mit einer Lösung von 5-10 µM Substanz in Ringer bei 2 µL/min durchgespült wurde (Figur 1). Am Ende dieser Phase wurde eine Probe entnommen (Probe 0). Anschließend folgte eine 57-Minütige Auswaschphase, wobei die Schläuche mit Ringerlösung gespült wurden, und alle 3 Minuten Proben gesammelt wurden. Für den Sondentest wurden die Materialien (N… Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (firstReferee), Prof. Dr. Andreas Baumann (furtherReferee), Prof. Dr. Hans Rommelspacher (furtherReferee).

Subjects/Keywords: Microdialysis; Analytical Methods; Pharmacokinetics, Drugs, Tubes, Membranes, Materials Testing (MESH), Mechanical Properties, In vitro; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Meier-Ince, B. v. (2008). In vitro Optimierung der Mikrodialyse für pharmakokinetische. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13808

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Meier-Ince, Beatrix von. “In vitro Optimierung der Mikrodialyse für pharmakokinetische.” 2008. Thesis, Freie Universität Berlin. Accessed January 19, 2021. https://refubium.fu-berlin.de/handle/fub188/13808.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Meier-Ince, Beatrix von. “In vitro Optimierung der Mikrodialyse für pharmakokinetische.” 2008. Web. 19 Jan 2021.

Vancouver:

Meier-Ince Bv. In vitro Optimierung der Mikrodialyse für pharmakokinetische. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 19]. Available from: https://refubium.fu-berlin.de/handle/fub188/13808.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meier-Ince Bv. In vitro Optimierung der Mikrodialyse für pharmakokinetische. [Thesis]. Freie Universität Berlin; 2008. Available from: https://refubium.fu-berlin.de/handle/fub188/13808

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

8. Giese, Christiane. Investigation of Biodegradation of Estrogens in the Aquatic Milieu.

Degree: 2006, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-7436

► Endocrine disruptors may adversely affect the health of animals and possibly that of humans. Compared to xenoestrogens, there has been little investigation of the significance… (more)

▼ Endocrine disruptors may adversely affect the health of animals and possibly that of humans. Compared to xenoestrogens, there has been little investigation of the significance of natural estrogens although the latter occur especially in the aquatic environment. The subject study investigates the changes in concentration of estrone and 17-estradiol in the aquatic milieu in the presence of an enzyme-immunoassay. The investigations are based on the hypothesis that estrogens outside an organism are microbiologically decomposed. The changes in concentration were investigated in river water and were compared to the changes in concentration in sterilized water. Samples of water containing estrone and 17-estradiol respectively were stored for 56 days at temperatures of 5 °C, 20 °C and 30 °C respectively. Furthermore, the influence of activated sludge and individual species of bacteria on estrogens in the water was considered. The study produced the following results: 1\. In river water, a pronounced decrease of the concentration of estrone was observed during the 56 days of storage. At 5 °C it took up to 42 days until more than 90 % of the initial concentration were decomposed. When stored at temperatures of 20 °C and 30 °C, however, 90 % of the initial concentration were decomposed after 2 to 14 days. The decomposition is interpreted as biodegradation. 2\. The decomposition of 90 % of the initial concentration of 17-estradiol took 4 to 12 days at a temperature of 20 °C. 17-estradiol is decomposed at a rate similar to that of estrone. 3\. During the 56 days of storage, the concentrations of estrone and 17-estradiol in aqua bidest. as well as in sterilized river water and in sterilized activated sludge remained at levels similar to the initial concentrations. The storage temperature did not affect the concentrations. Hence, non-biological factors which might cause a significant decomposition are ruled out. 4\. Activated sludge extracted from a sewage treatment plant accelerated the decomposition of estrone during storage. The microorganisms contained in the activated sludge are adapted to the utilization of estrone. 5\. The estrone concentration remained constant in the presence of Escherichia coli, Pseudomonas fluorescens or Aeromonas hydrophila. Being isolated from the other species, the bacteria are not able to biodegrade estrone under the given conditions. 6\. Several microtiter plates were used for one single experiment. Since every microtiter plate had to be initially calibrated, the same concentrations could lead to different concentration readings. To avoid errors, only one plate per experiment should be used. 7\. The curves of the estrogen concentration were described using different kinds of mathematical functions. For a logistic function, the sum of the square deviations is smaller than for an exponential or double-exponential function. Hence, the logistic function is suitable to be adapted to the data. This study contributes to the understanding of decomposition of estrogens in river waters. It therefore… Advisors/Committee Members: n (gender), Prof. Dr. G. Schlenker (firstReferee), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (furtherReferee), Prof. Dr. H. Tönhardt (furtherReferee).

Subjects/Keywords: biodegradation; oestrogens; estrone; estradiol; enzyme immunoassay; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giese, C. (2006). Investigation of Biodegradation of Estrogens in the Aquatic Milieu. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giese, Christiane. “Investigation of Biodegradation of Estrogens in the Aquatic Milieu.” 2006. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-7436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giese, Christiane. “Investigation of Biodegradation of Estrogens in the Aquatic Milieu.” 2006. Web. 19 Jan 2021.

Vancouver:

Giese C. Investigation of Biodegradation of Estrogens in the Aquatic Milieu. [Internet] [Thesis]. Freie Universität Berlin; 2006. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-7436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giese C. Investigation of Biodegradation of Estrogens in the Aquatic Milieu. [Thesis]. Freie Universität Berlin; 2006. Available from: http://dx.doi.org/10.17169/refubium-7436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

9. Hartje, Ilka. Effects of repeated Isoflurane anaesthesia with Xylazine/Polamivet® premedication on biochemical and physiological parameters and the fitness of Beagle-dogs.

Degree: 2006, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-6429

► In the study, the effects of repeated anaesthesia on selected parameters of general clinical examination (body weight, body temperature, respiratory rate and heart rate, cranial… (more)

▼ In the study, the effects of repeated anaesthesia on selected parameters of general clinical examination (body weight, body temperature, respiratory rate and heart rate, cranial nerve tests), the blood pressure, fitness (heart rate on defined ergometric load), haematological parameters and clinical chemistry (particularly liver and renal function tests) and the recovery behaviour were investigated in 1- to 2-year old Beagle-dogs. The effect of anaesthetic intervals and anaesthetic frequency on the investigated body functions and their significance for the well-being of the animal were of particular interest. The investigations presented here were conducted in dogs in which pharmacological investigations of ultrasound contrast agents from Schering AG diagnostics research were taking place in parallel over a prolonged period. The dogs repeatedly received premedication with Xylazine and Polamivet® and inhalation anaesthesia using Isoflurane. To evaluate the effect of the anaesthetic interval, a crossover experimental model was selected with 2 groups each consisting of 3 female Beagle-dogs and 2 different experimental phases. The animals in the first group were anaesthetised twice at an interval of 8 weeks in the first experimental phase, and the animals in the second group were anaesthetised 5 times at an interval of 2 weeks. In the second experimental phase this sequence was reversed between the groups. There was a 3-month break between the experimental phases. All other experimental data were described exploratively. The general clinical examination parameters, blood pressure and fitness were each recorded 1 day before anaesthesia and 24 hours, 7 and 14 days after anaesthesia. To examine renal and hepathic function in addition, 3 blood samples were taken during the anaesthesia (after premedication, 2 hours after induction of the inhalation anaesthesia and at the end of the anaesthesia). Furthermore, haematological and clinical chemistry investigations were each performed before and 2 weeks after each experimental phase. The recovery behaviour of the dogs following the anaesthesia was observed for up to 1 hour. The results of the crossover model showed a statistically significant difference in only one parameter with regard to the different anaesthetic intervals (liver enzyme ALP higher with anaestheisa at an interval of 8 weeks). The number of anaesthesia procedures had no effect on the parameters. However, some of the individual anaesthesia procedures had an effect on the recorded parameters predominantly 24 hours after the end of anaesthesia. These were apparent as weight loss, a rise in body temperature, an increase in liver enzyme activity (AST, ALT, ALP) and a reduced fitness. An effect on renal function cannot be fully excluded as 2 animals showed a temporary increase in serum urea or creatinine concentration over the reverences. The blood pressure remained unaffected. The effect of the anaesthesia procedures on the respiratory and heart rate was slight and not important. All of the effects of the anaesthesia… Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (firstReferee), Prof. Dr. M. Bräutigam (furtherReferee), Prof.%20Dr.%20N.-C.%20Juhr%22%29&pagesize-30">Univ.-Prof. Dr. N.-C. Juhr (furtherReferee).

Subjects/Keywords: inhaled anaesthetics; preanaesthetic medication; Isoflurane; Xylazine; adverse effects; dosage effects; renal function; liver function; Beagle; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hartje, I. (2006). Effects of repeated Isoflurane anaesthesia with Xylazine/Polamivet® premedication on biochemical and physiological parameters and the fitness of Beagle-dogs. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-6429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hartje, Ilka. “Effects of repeated Isoflurane anaesthesia with Xylazine/Polamivet® premedication on biochemical and physiological parameters and the fitness of Beagle-dogs.” 2006. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-6429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Hartje I. Effects of repeated Isoflurane anaesthesia with Xylazine/Polamivet® premedication on biochemical and physiological parameters and the fitness of Beagle-dogs. [Internet] [Thesis]. Freie Universität Berlin; 2006. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-6429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hartje I. Effects of repeated Isoflurane anaesthesia with Xylazine/Polamivet® premedication on biochemical and physiological parameters and the fitness of Beagle-dogs. [Thesis]. Freie Universität Berlin; 2006. Available from: http://dx.doi.org/10.17169/refubium-6429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

10. Rothe, Julia. Functions of the postsynaptic serotonin1A-receptor effects of antidepressants.

Degree: 2009, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-12623

► Depression is the most common psychiatric disorder in humans. The number of patients has been increasing for years which, in turn, rised the necessity to… (more)

▼ Depression is the most common psychiatric disorder in humans. The number of patients has been increasing for years which, in turn, rised the necessity to improve existing treatments and to develop new ones. For over fifty years the serotonergic system has been in focus of neuropharmacologic research with the aim of discovering the exact pathogenesis of depression. In the beginning an imbalance of transmitters was thought to be the cause for developing depression. More recent results, especially from PET-studies, showed changes in the concentration and binding potential of serotonin receptors in brain areas that have been linked with depression. Special attention has been paid to the 5-HT1A-receptor. It is notable by being expressed in two locations, presynaptic as a somatodendritic autoreceptor in the raphe and postsynaptic as a heteroreceptor in the areas of projections of serotonergic neurons, e.g. cortex and hippocampus. At our institute exists for the first time a mouse line overexpressing the 5-HT1A-receptor postsynaptically, mainly in cortex and hippocampus. Aim of this study was to investigate the role of the postsynaptic 5-HT1A-receptors for depression-like behaviour in these mice after treating them with antidepressants, that affect either the serotonergic or noradrenergic transmission system in two behavioural tests. Additionally the animals were tested in an animal model of depression. The behaviour of transgenic animals was compared with wild type mice behaviour. In order to test a stress resistance of the transgenic animals, as postulated by Bert et al. (2006), the reaction of mice to defined stress stimuli was measured by radiotelemetry. In a receptor autoradiography using [3H]-labeled 8-OH-DPAT, the distribution pattern of the 5-HT1A-receptor in transgenic and wild type mice as well as male and female mice was compared. In the Porsolt-swim test, a test to assess the antidepressant potential of a drug, non-treated transgenic mice behaved as if they had been treated with antidepressants. This was supported in the sucrose-preference test, a model that examines hedonistic impulses of the animals. After a chronic mild phase of stress, they still showed a constant level of hedonic behaviour and thus affirmed the „antidepressant“ phenotype at the Porsolt-swim test. The comparison of immobility times between the full (8-OH-DPAT) and partial 5-HT1A-agonist (buspirone) seemed to confirm the hypothesis that the overexpression led to a postsynaptic receptor reserve. Since both substances affect the animal´s locomotor activity, this could not be proved conclusively and needs further elucidation. Treatment with citalopram, a selective serotonin reuptake inhibitor (SSRI), caused decreased immobility times in both genotypes and sexes. The marked effect in transgenic animals underlines the role of 5-HT1A- receptors in the mediation of the antidepressant effect of Citalopram The effects of reboxetine, a selective noradrenalin reuptake inhibitor on the swimming behaviour of transgenic mice were surprising. The… Advisors/Committee Members: n (gender), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (firstReferee), Prof.%20Dr.%20N.-C.%20Juhr%22%29&pagesize-30">Univ.-Prof. Dr. N.-C. Juhr (furtherReferee), Prof.%20Dr.%20R.%20Morgenstern%22%29&pagesize-30">Univ.-Prof. Dr. R. Morgenstern (furtherReferee).

Subjects/Keywords: animal behavior; antidepressants; depression; sex differentiation; physical activity; serotonin; stress; swimming; 5HT1A receptor (MeSH); transgenic mice (MeSH); 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rothe, J. (2009). Functions of the postsynaptic serotonin1A-receptor effects of antidepressants. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-12623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rothe, Julia. “Functions of the postsynaptic serotonin1A-receptor effects of antidepressants.” 2009. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-12623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rothe, Julia. “Functions of the postsynaptic serotonin1A-receptor effects of antidepressants.” 2009. Web. 19 Jan 2021.

Vancouver:

Rothe J. Functions of the postsynaptic serotonin1A-receptor effects of antidepressants. [Internet] [Thesis]. Freie Universität Berlin; 2009. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-12623.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rothe J. Functions of the postsynaptic serotonin1A-receptor effects of antidepressants. [Thesis]. Freie Universität Berlin; 2009. Available from: http://dx.doi.org/10.17169/refubium-12623

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

11. Pirk, Gwenda. Presenting the drugs in palatable (sweet) fluids.

Degree: 2003, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-12473

► WOLFFGRAMM and HEYNE described for the first time in 1995 an animal model to induce behavioural dependence in rats. Other authors could not repeat their… (more)

▼ WOLFFGRAMM and HEYNE described for the first time in 1995 an animal model to induce behavioural dependence in rats. Other authors could not repeat their results (MÜLLER 2000). That is the reason why we investigated the influence of presenting alcohol and an opioid (etonitazene) in palatable (sweet) fluids to the induction of behavioural dependence on these drugs in rats in an animal model based on the results of WOLFFGRAMM et al. and MÜLLER. For all experiments male Wistar rats were used. In the first experiment the rats had the choice between two different alcohol concentrations (5% and 15%) in sweet fluids (strawberry or sugar taste) and tap water presented either all the time or every 24 hours for one day. After week 45 the alcohol was presented only in tap water until the end of the experiment (week 58). To check the development of behavioural depend-ence the alcoholic solutions were mixed with a bitter substance (quinine) or another bottle with the sweet fluid without alcohol was placed to the disposal. The consumed amount of alcohol decreased steadily. When the sweet fluid was replaced by tap water the decrease was even more impressive. After addition of quinine or presentation of another bottle with sweet fluid the rats nearly stopped the ingestion of the alcoholic solution. In the second experiment the rats were treated similar to those of the first experiment. They had the choice between a sweet alcoholic solution with a concentration of 20% and tap water over 32 weeks. After week 32 the alcohol was presented in tap water. From week 36 until the end (week 44) the concentration of the alcohol was increased to 30%. The consumption of alcohol decreased over the course of the trial and also every time the alcohol was mixed with quinine or another bottle with sweet fluid was presented. Altogether in both experiments none of the animals showed signs of behavioural dependence to alcohol during the course of the trial. For the third experiment raising concentrations of an opioid (etonitazene, ETZ) were pre-sented to the rats in sweet fluids. The schedule was similar to the other experiments. From week 25 to 28 the animals were deprived from the drug. After week 33 until the end (week 44) ETZ was soluted in tap water. The same tests as before to control the development of behav-ioural dependence were done. Until the weeks of drug deprivation the rats increased the consumed amount and decreased only few their drug intake during the control tests. But after replacement of the sweet fluid by tap water the rats showed an enormous and continuous decrease of the drug intake. Therefore even in this experiment with ETZ no behavioural dependence in the animals could be induced To conclude, we can say that even with some changes as the presentation of the drug in sweet fluid this animal model is not reliable to induce behavioural dependence in the rats. Advisors/Committee Members: n (gender), Prof.%20Dr.%20N.-C.%20Juhr%22%29&pagesize-30">Univ.-Prof. Dr. N.-C. Juhr (firstReferee), Prof.%20Dr.%20A.%20Baumgartner%22%29&pagesize-30">Univ.-Prof. Dr. A. Baumgartner (furtherReferee), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (furtherReferee).

Subjects/Keywords: Rats; Alcohol Intake; Opioids; Addiction; Palatability; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pirk, G. (2003). Presenting the drugs in palatable (sweet) fluids. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-12473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pirk, Gwenda. “Presenting the drugs in palatable (sweet) fluids.” 2003. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-12473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pirk, Gwenda. “Presenting the drugs in palatable (sweet) fluids.” 2003. Web. 19 Jan 2021.

Vancouver:

Pirk G. Presenting the drugs in palatable (sweet) fluids. [Internet] [Thesis]. Freie Universität Berlin; 2003. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-12473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pirk G. Presenting the drugs in palatable (sweet) fluids. [Thesis]. Freie Universität Berlin; 2003. Available from: http://dx.doi.org/10.17169/refubium-12473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

12. Frank, Cornelia. Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour.

Degree: 2009, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/13384

► The aim of the present study was the development of a behaviour test for dogs suitable for the everyday life in an animal shelter. In… (more)

▼ The aim of the present study was the development of a behaviour test for dogs suitable for the everyday life in an animal shelter. In addition to the revelation of behavioural problems as a main criterion for the placement options of a dog, possible changes of the behaviour during a prolonged stay in the animal shelter should be recognised. As a baseline, dogs were tested in their kennel using 12 subtests. The content of these subtests are situations close to reality, which have proven their predictive value in other behaviour tests to some extent. Dogs were evaluated according to the scale developed by NETTO and PLANTA [1997] which is based on a 5 point Gutman-scale [DE VELLIS 1991]. Predominantly dominant and scare based aggressive behaviour was recorded using a special form. As a guideline for the evaluation of a dog by the tester, a list of character features was used. Statements on the placement options of a dog were made taking the highest level determined using the modified scale into account. Starting with the baseline test altogether 70 dogs were investigated within the first 5 days after their arrival in one of the 3 animal shelters participating in this study. Only about 27% of the dogs investigated showed aggressive or scare based aggressive behaviour of a level > 1. Predictions on potential behavioural problems of the dogs investigated are possible to a certain extent, but limited by the need of practicability during the everyday life in an animal shelter. Based on their behaviour 14.3% of the dogs investigated were classified non placeable. About 4 weeks after the baseline test the identical test was repeated using 23 dogs which remained in the animal shelter. 17.4% of these dogs showed conspicuous behaviour. Direct comparison of all subtests of the baseline and the repeated tests as well as of the several subtests of the dogs which were investigated repeatedly, showed a positive change of behaviour. Only the direct comparison of the subtest “turning onto the back” was an exemption. During the repeated test an increase of aggressive behaviour against the tester was observed. Advisors/Committee Members: n (gender), Prof.%20Dr.%20N.-C.%20Juhr%22%29&pagesize-30">Univ.-Prof. Dr. N.-C. Juhr (firstReferee), Prof.%20Dr.%20H.%20Fink%22%29&pagesize-30">Univ.-Prof. Dr. H. Fink (furtherReferee), Prof.%20Dr.%20E.%20Schein%22%29&pagesize-30">Univ.-Prof. Dr. E. Schein (furtherReferee).

Subjects/Keywords: dogs; animal behaviour; aggressive behaviour; shelters; kennels; adoption; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA (6^th Edition):

Frank, C. (2009). Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13384

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Frank, Cornelia. “Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour.” 2009. Thesis, Freie Universität Berlin. Accessed January 19, 2021. https://refubium.fu-berlin.de/handle/fub188/13384.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Frank, Cornelia. “Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour.” 2009. Web. 19 Jan 2021.

Vancouver:

Frank C. Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour. [Internet] [Thesis]. Freie Universität Berlin; 2009. [cited 2021 Jan 19]. Available from: https://refubium.fu-berlin.de/handle/fub188/13384.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Frank C. Development and implementation of a behaviour test for dogs kept in animal shelters and investigation of changes in their behaviour. [Thesis]. Freie Universität Berlin; 2009. Available from: https://refubium.fu-berlin.de/handle/fub188/13384

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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