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You searched for +publisher:"Freie Universität Berlin" +contributor:("Prof. Dr. sc. med. Wolfgang Presber"). Showing records 1 – 2 of 2 total matches.

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Freie Universität Berlin

1. Schlafer, Sebastian. molecular epidemiology and spatial distribution in biofilms.

Degree: 2012, Freie Universität Berlin

Periodontitis is an inflammatory disease of bacterial aetiology that affects all structures supporting and anchoring the tooth. In the adult population, periodontitis represents the most frequent cause of tooth loss. The microflora in periodontal lesions forms complex, structurally heterogeneous biofilms on the root surface of affected teeth and comprises a multitude of fastidious and as yet uncultured bacteria. Identification of these organisms was only made possible by the advent of culture-independent DNA-based techniques. Filifactor alocis is a fastidious, Gram-positive rod and was repeatedly detected in patients suffering from chronic periodontitis (CP) and generalized aggressive periodontitis (GAP) in recent years. For the first study of the present dissertation, a species-specific oligonucleotide probe was designed, and dot blot hybridizations were performed to determine the prevalence of F. alocis in a total of 490 subgingival samples from GAP patients, CP patients and periodontitis-resistant (PR) patients. Furthermore, fluorescence in situ hybridization (FISH) and subsequent epifluorescence microscopy were used to analyse the spatial distribution and structural arrangement of F. alocis in subgingival biofilms from GAP patients. In the second study, identical methods were employed to determine the prevalence of oral Selenomonas spp., regarded as candidate periodontal pathogens by some authors, in 742 samples from GAP, CP and PR patients. Moreover, the structural arrangement of these organisms in GAP biofilms was studied. F. alocis proved to be an excellent marker organism for periodontal disease. The species could be detected in the majority of CP and GAP patients and had an extremely low prevalence in the PR group. The microscopic analysis of subgingival biofilms from GAP patients suggested an important role of F. alocis in the pathogenesis of periodontal disease. The organism predominantly colonized the depth of periodontal pockets and contributed to numerous bacterial formations that reflect a structural organization of the biofilms. In contrast, the prevalence of Selenomonas spp. was not correlated with periodontal disease. However, FISH and subsequent microscopic analysis of GAP biofilms showed that, at least in some cases, Selenomonas spp. appeared in large numbers and made a relevant contribution to biofilm architecture. In the third study of the present work, biopsies from digital dermatitis (DD) lesions, an ulcerative inflammatory bovine foot disease, were analysed. With the help of FISH, Guggenheimella bovis, a Gram- positive coccoid rod that is only seldom detected in DD-lesions, could be visualized deep inside the tissue as a pioneer organism way ahead of the advancing bacterial front. The present work clearly demonstrates the advantages of a combined epidemiological and microscopic approach for the study of polymicrobial biofilm diseases. Advisors/Committee Members: [email protected] (contact), m (gender), PD Dr. med. Annette Moter (firstReferee), Prof. Dr. sc. med. Wolfgang Presber (furtherReferee), PD Dr. med. Dirk Theegarten (furtherReferee).

Subjects/Keywords: Biofilm; digital dermatitis; dot blot hybridization; FISH; periodontitis; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Schlafer, S. (2012). molecular epidemiology and spatial distribution in biofilms. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-12753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Schlafer, Sebastian. “molecular epidemiology and spatial distribution in biofilms.” 2012. Thesis, Freie Universität Berlin. Accessed December 06, 2019. http://dx.doi.org/10.17169/refubium-12753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Schlafer, Sebastian. “molecular epidemiology and spatial distribution in biofilms.” 2012. Web. 06 Dec 2019.

Vancouver:

Schlafer S. molecular epidemiology and spatial distribution in biofilms. [Internet] [Thesis]. Freie Universität Berlin; 2012. [cited 2019 Dec 06]. Available from: http://dx.doi.org/10.17169/refubium-12753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schlafer S. molecular epidemiology and spatial distribution in biofilms. [Thesis]. Freie Universität Berlin; 2012. Available from: http://dx.doi.org/10.17169/refubium-12753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Pauli, Daniela. Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans.

Degree: 2006, Freie Universität Berlin

The conversion of glucosamine-6-phosphate into fructose-6-phosphate catalyzed by the enzyme glucosamine-6-phosphate deaminase (GNPDA) is an important step in the N-acetyl-glucosamine (GlcNAc) and glucosamine (GlcN) catabolic pathway. Normally, C. albicans strains are able to utilize these two aminosugars as the sole carbone source. The work group of Tietz described morphologically atypical C. albicans strains from Africa which seemed to be less virulent than typical C. albicans strains from the same geographical area [Tietz et al., 1995]. The objective of this study was to search for mutations in the NAG1 gene, which the GNPDA encodes, or for changes in the gene regulation that may explain the inability of GlcNAc- and GlcN-negative strains to assimilate the two amino sugars. The ability to induce GNPDA through GlcNAc is reduced in the atypical strains. However, in all uninduced strains, typical and atypical, an activity of GNPDA was detectable. Specific PCR primers, based on sequences of the NAG1 gene published by Natarajan and Datta (1993), were designed for the amplification of the NAG1 gene DNA and mRNA in various typical and atypical C. albicans strains. The analysis of the gene-expression showed no significant differences in the concentrations of mRNA in both populations (GlcNAc induced and not induced). To test for sequence polymorphisms, the amplification products were subjected to an sscp analysis. All GlcNAc- and GlcN-negative strains with the exception of two strains had an identical but unique sscp pattern, whereas all typical strains tested showed varying, almost individual sscp patterns. Sequencing of the amplified NAG1 genes of GlcNAc- and GlcN- negative strains did reveal three mutations in the amino acid sequence compared to the gene amplified from typical strains, which could provoke a change of conformation in the enzyme and therefore a reduced induction may be the consequence. Advisors/Committee Members: n (gender), Prof. Dr. sc. med. Wolfgang Presber (firstReferee), Prof. Dr. med. Reinhard RĂ¼chel (furtherReferee), Prof. Dr. med. Michael Seyfarth (furtherReferee).

Subjects/Keywords: <; i>; C. albicans<; /i>;

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pauli, D. (2006). Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7051

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Pauli, Daniela. “Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans.” 2006. Thesis, Freie Universität Berlin. Accessed December 06, 2019. http://dx.doi.org/10.17169/refubium-7051.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Pauli, Daniela. “Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans.” 2006. Web. 06 Dec 2019.

Vancouver:

Pauli D. Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans. [Internet] [Thesis]. Freie Universität Berlin; 2006. [cited 2019 Dec 06]. Available from: http://dx.doi.org/10.17169/refubium-7051.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pauli D. Molecular characterization of glucosamine-6-phosphate deaminase (NAG1) from Candida albicans. [Thesis]. Freie Universität Berlin; 2006. Available from: http://dx.doi.org/10.17169/refubium-7051

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.