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You searched for +publisher:"Freie Universität Berlin" +contributor:("Prof. Dr. med. Petra Reinke"). Showing records 1 – 2 of 2 total matches.

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Freie Universität Berlin

1. Meyer, Sonja. A new strategy for adoptive immunotherapy.

Degree: 2006, Freie Universität Berlin

Adoptive immunotherapy with antigen-specific T-cells has been successfully used to treat certain infectious diseases and cancers. The frequent use of T -cell-therapy is restricted by limitations in current T cell generation strategies. The most commonly applied peptide-based approaches rely on the knowledge of relevant epitopes. Therefore, T-cells cannot be generated for diseases with unknown epitopes or for patients with unfavorable HLA types, and the transfer under GMP-guidelines is critical. We developed a peptide-based approach for HLA type-independent generation of specific T cells against various proteins. It is based on short-time stimulation with peptide libraries that cover most CD4+ and CD8+ T cell epitopes of given proteins. The procedure requires no prior knowledge of epitopes because libraries are synthesized solely on the basis of the protein's amino acid sequence. Using HCMV as an example, we generated pp65-specific T-Cells by short-time-stimulation with the overlapping peptide library of pp6, followed by immunomagnetic selection of activated IFN-g-secreting cells and nonspecific expansion. Generated T cell lines consisted of pp65-specific CD4+ and CD8+ lymphocytes that displayed antigen-specific killing and proliferation. The protocol combines the biosafety of peptide-based approaches with HLA type independence and may help to advance adoptive immunotherapy in the future. Advisors/Committee Members: n (gender), Prof. Dr. med. Petra Reinke (firstReferee), Prof. Dr. med. D. Kabelitz (furtherReferee), Prof. Dr. med. H. Einsele (furtherReferee).

Subjects/Keywords: Adoptive immunotherapy; peptide libraries; CMV; T-cells; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Meyer, S. (2006). A new strategy for adoptive immunotherapy. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Meyer, Sonja. “A new strategy for adoptive immunotherapy.” 2006. Thesis, Freie Universität Berlin. Accessed December 07, 2019. https://refubium.fu-berlin.de/handle/fub188/13960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Meyer, Sonja. “A new strategy for adoptive immunotherapy.” 2006. Web. 07 Dec 2019.

Vancouver:

Meyer S. A new strategy for adoptive immunotherapy. [Internet] [Thesis]. Freie Universität Berlin; 2006. [cited 2019 Dec 07]. Available from: https://refubium.fu-berlin.de/handle/fub188/13960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meyer S. A new strategy for adoptive immunotherapy. [Thesis]. Freie Universität Berlin; 2006. Available from: https://refubium.fu-berlin.de/handle/fub188/13960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Bold, Gantuja. Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression.

Degree: 2008, Freie Universität Berlin

Pharmacodynamic test and methods analyzing the functional effect of immunosuppressive drugs on the immune response might be more informative for the optimization of an individualized immunosuppressive therapy if compared to pharmacodynamic parameters. For this reason pharmacodynamic tests are needed for endpoints of intracellular pathways in lymphocytes and monocytes, mirroring the net effect of different immunosuppressive drugs on the immune response being responsible for graft rejection. Examples of pathway endpoints are lymphocyte proliferation, expression of T-cells surface markers and cytokine production by T-cells and monocytes. Here I evaluated standardized whole blood functional tests for lymphocytes and monocytes after in vitro mitogen stimulation using a new semiautomatic ELISA to monitor immuno- suppressant drugs in renal transplant patients. The objective was the early detection of suboptimal immunosuppression harbouring the risk of renal graft rejection and the detection of supraoptimal immunosuppression with an increased infection risk. A major prediction factor for renal graft rejection is the quality of the kidney to be transplanted. The immunogenicity of the graft is supposedly influenced by donor characteristics like age, hypertension, graft ischemia and reperfusion injury, brain death and others. The increasing use of marginal-suboptimal donor organs as a solution for organ shortage contributes to these problems. However, tests to reliably evaluate individual graft quality are needed. A possible solution is the preoperative monitoring of the inflammatory response by quantification of key cytokines in urine and blood plasma. Urine cytokine quantification is a simple non-invasive method, probably mirroring the graft immunological situation most reliably. Here I could show that increased IL-8 and TNF-alpha urin level during early post transplantation period predicted an acute renal graft rejection. It is believed that increased graft immunogenicity might be induced by pronounced ischemia and reperfusion injury of the graft. Further studies will focus on this hypothesis. Advisors/Committee Members: n (gender), Prof. Dr. med. Petra Reinke (firstReferee), Prof. Dr. med. Dr. h.c. U. Heemann (furtherReferee), Prof. Dr. med. W. Schareck (furtherReferee).

Subjects/Keywords: kindney transplantation; immuno-monitoring; graft rejection; IL-8; TNF-alpha; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bold, G. (2008). Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-12755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bold, Gantuja. “Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression.” 2008. Thesis, Freie Universität Berlin. Accessed December 07, 2019. http://dx.doi.org/10.17169/refubium-12755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bold, Gantuja. “Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression.” 2008. Web. 07 Dec 2019.

Vancouver:

Bold G. Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2019 Dec 07]. Available from: http://dx.doi.org/10.17169/refubium-12755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bold G. Standardized immuno-monitoring during early period after kidney transplantation for pharmacodynamic detection of over- and under-suppression. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-12755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.