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You searched for +publisher:"Freie Universität Berlin" +contributor:("Prof. Dr. Th. Stamminger"). One record found.

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Freie Universität Berlin

1. Deckers, Jonas Merlin S. Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus.

Degree: 2009, Freie Universität Berlin

Cytomegalovirus infections can cause serious problems in fetus, newborns and immunocompromised patients, and viral DNA is usually detected with specific PCR. An unprejudiced way of analysing human samples for the presence of known and unknown cytomegalovirus genotypes is to use degenerate primers. However, in mixed infections, only one dominant genotype is usually amplified. Here, we present a modification of degenerate nested PCR by utilizing oligonucleotides of locked nucleic acids (LNA) to specifically block the genotype sequence which is preferentially amplified in the PCR reaction. This technology was used to detect and differentiate genotypic variants of the glycoprotein B gene (UL55) and a G-protein-coupled receptor gene (UL33) of human cytomegalovirus (HCMV). Two nested degenerate primer pairs were designed which broadly amplified hominoid CMV (from men and chimpanzees). With the help of UL55- and UL33-specific LNA, a collection of clinical samples was differentially screened for the occurrence of multiple infections with HCMV genotypes. The five gB and the three UL33 genotypic variants known to date were identified, and a novel fourth UL33 genotype was discovered. Advisors/Committee Members: [email protected] (contact), n (gender), Prof. Dr. med. D.H. Krüger (firstReferee), Priv.-Doz. Dr. med. W. Brune (furtherReferee), Prof. Dr. Th. Stamminger (furtherReferee).

Subjects/Keywords: cytomegalovirus; CMV; glycoprotein B; UL55; UL33; G-protein-coupled receptor; primate; gorilla; chimpanzee; genotype; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Deckers, J. M. S. (2009). Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-4301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Deckers, Jonas Merlin S. “Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus.” 2009. Thesis, Freie Universität Berlin. Accessed December 10, 2019. http://dx.doi.org/10.17169/refubium-4301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Deckers, Jonas Merlin S. “Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus.” 2009. Web. 10 Dec 2019.

Vancouver:

Deckers JMS. Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus. [Internet] [Thesis]. Freie Universität Berlin; 2009. [cited 2019 Dec 10]. Available from: http://dx.doi.org/10.17169/refubium-4301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Deckers JMS. Variability of a glycoprotein and a G-protein-coupled receptor (UL55, UL33) of cytomegalovirus. [Thesis]. Freie Universität Berlin; 2009. Available from: http://dx.doi.org/10.17169/refubium-4301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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