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You searched for +publisher:"Freie Universität Berlin" +contributor:("Prof. Dr. Christoph Benning"). One record found.

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Freie Universität Berlin

1. Essigmann, Bernd. Biosynthesis, Regulation and Function.

Degree: 1999, Freie Universität Berlin

The sulfolipid sulfoquinovosyldiacylglycerol is found in almost all photosynthetic and few non-photosynthetic organisms. Studies on the biosynthesis and function of this unusual lipid were performed mostly in bacteria. In the present work the higher plant Arabidopsis thaliana was used as model organism. One part of this study focused on the biosynthesis of sulfolipid, in particular the formation of the sulfoquinovosyl-headgroup. Therefor a genetic approach was chosen. The SQD1 gene of Arabidopsis was expressed in E. coli and purified. The SQD1 protein was first characterized using a structural model and the results were proven biochemical. It was possible to identify NAD+ as cofactor and UDP-Glucose as substrate of SQD1. Crystallization and the resulting structure of SQD1confirmed the structural model and the biochemical characterization. Considering all these results a reaction mechanism for the SQD1 protein was proposed. In the second part of this work the regulation and functions of sulfolipid in Arabidopsis were investigated. Under phosphate-limiting conditions drastic changes in the lipid composition as well as strong induction of SQD1 mRNA and SQD1 protein were observed. Furthermore the pho1 mutant of Arabidopsis was used for physiological experiments. In spite of dramatic changes in the lipid composition this mutant shows no changes in the light acclimation in comparison to the wild type. It is suggested that adjustments of lipid composition of thylakoid membranes allow the cell to maintain the function of essential processes that are restricted to these membranes, such as photosynthesis. In particular a certain amount of anionic thylakoid lipid is maintained by substituting sulfolipid for phosphatidylglycerol under reduced phosphate availability. Advisors/Committee Members: n (gender), Prof. Dr. Volker A. Erdmann (firstReferee), Prof. Dr. Christoph Benning (furtherReferee).

Subjects/Keywords: plant; membrane lipids; photosynthesis; UDP-sulfoquinovose; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Essigmann, B. (1999). Biosynthesis, Regulation and Function. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Essigmann, Bernd. “Biosynthesis, Regulation and Function.” 1999. Thesis, Freie Universität Berlin. Accessed August 06, 2020. https://refubium.fu-berlin.de/handle/fub188/11127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Essigmann, Bernd. “Biosynthesis, Regulation and Function.” 1999. Web. 06 Aug 2020.

Vancouver:

Essigmann B. Biosynthesis, Regulation and Function. [Internet] [Thesis]. Freie Universität Berlin; 1999. [cited 2020 Aug 06]. Available from: https://refubium.fu-berlin.de/handle/fub188/11127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Essigmann B. Biosynthesis, Regulation and Function. [Thesis]. Freie Universität Berlin; 1999. Available from: https://refubium.fu-berlin.de/handle/fub188/11127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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