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You searched for +publisher:"Freie Universität Berlin" +contributor:("Priv.-Doz. Dr. rer. nat. Th. Schulz"). Showing records 1 – 3 of 3 total matches.

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Freie Universität Berlin

1. Föst, Agnes. Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3.

Degree: 2011, Freie Universität Berlin

Linezolid is a antibiotic from the group oxazolidinone. It is used against grampositive pathogens. In trials with animals it shows effectson the fertility. The quinolones is a group of often used antibiotics. They also sohws effects on fertility and reproductive skills. The experiments have done for 3 and 7 days in the concentrations of antibiotics from 0,1 to 100 mg/l. All experiments were done with the sertoli-cellline SerW3. The effects of the proteins of the blood-testis-barrier, Occludin, N-Cadherin, β1-Integrine,Vimentin and Connexin 43 were analysiered with Western-Blot- Analysis, Immunhistology and Microscopy. The results show that Linezolid has the biggest effect in low concentrations on the SerW3-cells. The effects on the proteins Occludin, N-Cadherin and Connexin 43 are obviously. Advisors/Committee Members: w (gender), Prof. Dr. med. R. Stahlmann (firstReferee), Prof. Dr. med. B. Drewelow (furtherReferee), Priv-Doz. Dr. rer. nat. Th. Schulz (furtherReferee).

Subjects/Keywords: Linezolid; Levofloxacin; Moxifloxacin; SerW3; fertility; Sertoli cells; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Föst, A. (2011). Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/10696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Föst, Agnes. “Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3.” 2011. Thesis, Freie Universität Berlin. Accessed December 13, 2019. https://refubium.fu-berlin.de/handle/fub188/10696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Föst, Agnes. “Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3.” 2011. Web. 13 Dec 2019.

Vancouver:

Föst A. Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2019 Dec 13]. Available from: https://refubium.fu-berlin.de/handle/fub188/10696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Föst A. Effects of Linezolid, Levofloxacin and Moxifloxacin on the cells of the Sertoli-cellline SerW3. [Thesis]. Freie Universität Berlin; 2011. Available from: https://refubium.fu-berlin.de/handle/fub188/10696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Zurek, Martin. Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3.

Degree: 2011, Freie Universität Berlin

Di-(2-ethylhexyl)-phthalat (DEHP) is a synthetic plasticiser. It will be metabolized in the gastrintestinal tract in the active metabolite Mono-(2-ethylhexyl)-phthalat (MEHP). The heavy metal cadmium is widely spread, for example in food. It ist known, that all substances have a high effect on fertility. The experiments have done for 1, 3 and 6 days in the concentrations of phthalates from 0,01 μΜ to 100 μΜ and of cadmium from 0,01 μΜ to 1 μΜ. All experiments were done with the sertoli-cellline SerW3. The effects of the proteins of the blood-testis-barrier, Occludin, N-Cadherin, β1-Integrine,Vimentin and Connexin 43 were analysiered with Western-Blot- Analysis, Immunhistology and Microscopy. The results show that MEHP has the biggest effect in low concentrations on the SerW3-cells. The effects on the proteins Occludin, N-Cadherin and Connexin 43 are obviously. Advisors/Committee Members: m (gender), Prof. Dr. med. R. Stahlmann (firstReferee), Prof. Dr. P. Steinberg (furtherReferee), Priv-Doz. Dr. rer. nat. Th. Schulz (furtherReferee).

Subjects/Keywords: phthalates; SerW3; DEHP; MEHP; cadmium; sertoli cells; fertility; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Zurek, M. (2011). Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Zurek, Martin. “Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3.” 2011. Thesis, Freie Universität Berlin. Accessed December 13, 2019. https://refubium.fu-berlin.de/handle/fub188/13865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Zurek, Martin. “Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3.” 2011. Web. 13 Dec 2019.

Vancouver:

Zurek M. Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2019 Dec 13]. Available from: https://refubium.fu-berlin.de/handle/fub188/13865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zurek M. Effects of the Phthalates DEHP [Di-(2-ethylhexyl)-phthalat] and MEHP [Mono-(2-ethylhexyl)-phthalat] and of the heavy metal Cadmium on the cells of the Sertoli-cellline SerW3. [Thesis]. Freie Universität Berlin; 2011. Available from: https://refubium.fu-berlin.de/handle/fub188/13865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

3. Kaltenhäuser, Johanna. Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test.

Degree: 2006, Freie Universität Berlin

The aim of the presented thesis was to study gene expression during cardiomyocyte differentiation in embryonic stem cells and to establish molecular markers for embryotoxic substance effects after selection of appropriate candidate genes. The work was conducted on the basis of the Embryonic Stem Cell Test (EST), an alternative method to animal experiments, which is intended for the prediction of an embryotoxic potential of substances (Spielmann et al., 1997). The EST is based on the differentiation of embryonic stem cells of the mouse (line D3). Their ability to form contracting cardiomyocytes within 10 days of development is used as a model of embryonic development. During the differentiation period the expression of 18 genes with a known in vivo function in early mesoderm or heart differentiation was studied by means of quantitative RT-PCR. This selection contained heart muscle specific genes of the contractile apparatus alpha-MHC, beta-MHC, MLC1, MLC2v, alpha-Actin, alpha-Actinin and Troponin T, transcription factors of early mesodermal and heart development GATA-4, Tbx5, Hand1 and 2, MesP1 and 2, as well as myocardin, the cytokine cardiotrophin-1, the growth factor cripto-1 and, in addition, genes, which are not heart specific (Oct4 and NF L). During the differentiation of D3 cells to cardiomyocytes, the majority of gene specific transcripts was found in patterns that were in accordance with their expression in vivo. The expression of important transcription factors like MesP1 and 2 as well as Hand1 and 2 was determined during in vitro cardiomyocyte differentiation in D3 cells for the first time. In order to establish molecular markers for a prediction of embryotoxic effects on the level of gene expression, the genes MesP1 and MLC1 were chosen as test candidates, whose expression was determined after substance treatment at appropriate time points (MesP1 on day 5; MLC1 on day 7 and in addition on day 10). In substance tests with 11 substances with known embryotoxic potential in vivo and two substances without comprehensive in vivo data, it could be shown that the sensitivity of the molecular markers was comparable with the conventional microscopic endpoint in the majority of cases. An analysis of the results of the 11 known substances with all four endpoints using a mathematic model which was developed for the prediction of an embryotoxic potential (Genschow et al., 2002) lead in all but two single tests to the correct classification. Thus, out of the molecular markers that were established successfully, two lead to a correct prediction of the embryotoxic potential of the substances even earlier than the conventional endpoint. The results of the presented work contribute to understanding the succession of molecular events during in vitro differentiation of cardiomyocytes and, therefore, to improve the possibilities of the EST to be applied as a screening test for embryotoxic effects in early substance development. The results allow to shorten the test duration and with the use of molecular endpoints… Advisors/Committee Members: n (gender), Prof. Dr. med. G. Schönfelder (firstReferee), Priv.-Doz. Dr. rer. nat. Th. Schulz (furtherReferee).

Subjects/Keywords: Embryonic stem cell test; cardiomyocyte differentiation; in-vitro-method; qRT-PCR; embryotoxic substances; molecular markers; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kaltenhäuser, J. (2006). Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Kaltenhäuser, Johanna. “Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test.” 2006. Thesis, Freie Universität Berlin. Accessed December 13, 2019. http://dx.doi.org/10.17169/refubium-7301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Kaltenhäuser, Johanna. “Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test.” 2006. Web. 13 Dec 2019.

Vancouver:

Kaltenhäuser J. Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test. [Internet] [Thesis]. Freie Universität Berlin; 2006. [cited 2019 Dec 13]. Available from: http://dx.doi.org/10.17169/refubium-7301.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaltenhäuser J. Establishment of molecular markers as part of the advancement of an in vitro embryotoxicity test. [Thesis]. Freie Universität Berlin; 2006. Available from: http://dx.doi.org/10.17169/refubium-7301

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.