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You searched for +publisher:"Freie Universität Berlin" +contributor:("Priv.-Doz. Dr. Kerstin Borchers"). Showing records 1 – 2 of 2 total matches.

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Freie Universität Berlin

1. Heinemann, Daniela. Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood.

Degree: 2008, Freie Universität Berlin

EHV-1/-4 infections in horses not only cause severe clinical symptoms, but also economical losses. Vaccination plays an important role in preventing EHV-1/-4 infections. Vaccines currently available on market usually only stimulate the humorale immune response. The aim of the present study was to evaluate whether it is possible to improve the humoral immune response and whether the cellular immune response could be stimulated by the combination of the inactivated vaccine Resequin® NN plus and the immune modulator Zylexis®. A group of 20 horses were divided into a Zylexis-group (n = 11) and a Placebo- group (n = 9). The animals of both groups received three applications of Zylexis® or a Placebo respectively at 48 hour intervals. The horses were vaccinated with the inactivated combination vaccine Resequin® NN plus simultaneously to the third Zylexis®-/Placebo application. The examination of the immune response was carried out at 6 different time points. Initial values were determined 30 days before the beginning of the study. Blood samples were collected 6 hours after the second and third combined application of Zylexis®-/Placebo and Resequin® NN plus. Further blood samples were collected on days 3, 7 and 14 after vaccination. At every time point of investigation the antibody titer against EHV-1 and EHV-4 was measured with neutralization test and indirect immunofluorescence test, the number of peripheral mononuclear blood cells and the cytokine gene expression of IFN-γ, IL-2, IL-4 and IL-10 were examined. The immune status was determined at two time points (day -30 and day 0) by LABOKLIN. Foremost we studied the gene expression of selected cytokines using quantitative “real-time” TaqMan®-PCR. For the cellular immune response the gene expression levels of IFN-γ and IL 2 and for the humoral immune response the levels of gene expression of IL-4 and IL-10 were used as parameters. The cytokine mRNA was analyzed with TaqMan®-PCR directly from the horses’ blood (ex vivo) and for comparison after stimulation with Concavalin A for a period of 24 hours (in vitro). We noticed that in vivo examinations of cytokine mRNA using TaqMan®-PCR is sufficient to detect differential gene expressions. The results showed that the administration of Zylexis® in combination with the vaccine Resequin® NN plus resulted in significantly increased numbers of equine PBMCs. Further more the induction of the gene expression of IL-4 and IL-10 after vaccination equally mirrored an activity of the humoral immune response in the Zylexis®- and Placebo-treated horses. These findings correlate with the serological reactions of EHV-1/-4 antibodies. Thus the IFT and NT antibody titers increased in 19 (95%) animals and 7 (35%) horses respectively. The virus neutralizing antibodies could be detected especially in horses aged at least 4 years. No significant differences regarding the level of virus specific antibody titers and cytokine gene expression were noted between the Zylexis- and Placebo-groups. An induction of the IFN-γ and IL-2 in the present study could… Advisors/Committee Members: n (gender), Priv.-Doz. Dr. Kerstin Borchers (firstReferee), Univ.-Prof. Dr. Michael F. G. Schmidt (furtherReferee), Univ.-Prof. Dr. Kerstin E. Müller (furtherReferee).

Subjects/Keywords: horses; equine herpesvirus; vaccination; cytokines; immunostimulation; immune response; polymerase chain reaction; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Heinemann, D. (2008). Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-4819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Heinemann, Daniela. “Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood.” 2008. Thesis, Freie Universität Berlin. Accessed August 04, 2020. http://dx.doi.org/10.17169/refubium-4819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Heinemann, Daniela. “Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood.” 2008. Web. 04 Aug 2020.

Vancouver:

Heinemann D. Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2020 Aug 04]. Available from: http://dx.doi.org/10.17169/refubium-4819.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heinemann D. Ex vivo and in vitro studies of the impact of Zylexis® on the immune response following vaccination with Resequin® NN plus with special consideration of the EHV-1/-4 specific antibody response, the immune status and the cytokine gene expression patterns in horse blood. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-4819

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Fetsch, Alexandra. EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors.

Degree: 2007, Freie Universität Berlin

Several times, an involvement of the Equine Herpesvirus Type 2 (EHV-2) in different forms of inflammatory diseases of the cornea and/or conjunctiva in horses is documented; the exact pathogenesis of equine keratoconjunctivitis has yet not been understood completely. This project focus on the question, whether EHV-2-infected eye-diseased horses possibly show an immune status which differs from the reference value. Besides, the results of this thesis should provide conclusions, if the pathogenesis of the disease is possibly been influenced by immunosuppressive mechanisms of EHV-2. Furthermore, it should be investigated, whether other viral and/or bacterial pathogens play a role in equine keratoconjunctivitis. Finally, the investigations of the ocular tissue- and cell-tropism of EHV-2/-5 should further contribute to the understanding of the pathogenesis of this disease. Investigations of the cellular immune status had been carried out comparing ten eyediseased and 21 clinically sound horses. PBL, isolated from blood samples and swabs from the conjunctival fossa had been investigated using a EHV-2 specific nested PCR. Additionally, serological investigations had been carried out and the cellular immune status of each horse had been determined using flow cytometry and white blood cell count methods. EHV-2 was detected within this study in both, eye- diseased and clinically sound horses by serological methods respectively nPCR. However, a statistically significant correlation between EHV-2 and the characteristic eye-diseased was not observed. Referring to the cellular immune status EHV-2 positive horses without ocular diseases showed significantly more often decreased b-cell numbers than EHV-2 negative horses with and without ocular diseases, respectively. This should be seen as first indication that an EHV-2 infection or reactivation causes a result in a measurable reduction of b-cells. Due to the small sample number, only further investigations will give information about, how individual differences of the immune system may influence the outbreak of keratoconjunctivitis induced by EHV-2. To answer the question, whether other co-factors such as viral or bacterial pathogens are involved in EHV-2 induced equine keratoconjunctivitis, eye-swabs and blood samples from a second investigation group (consisting of 68 eye-diseased and 32 clinically sound horses) had been tested for EHV-2 and subsequently EHV-5, using specific nPCRs. Finally, random samples of eye-swabs had been investigated using pathogen specific PCRs for the presence of EAdV-1, chlamydiae and mycoplasmae, respectively. Within the scope of this project, the EAdV-1 specific nPCR had been established previously. A statistically significant correlation between eye-diseased and sound horses relating to the detection rate of EHV-2 in swab- and blood-samples was also not observed in this investigation group. In addition, evidence for an involvement of EAdV-1, chlamydiae and mycoplasmae, respectively in inflammatory diseases of the cornea and/or conjunctiva of horses… Advisors/Committee Members: n (gender), Priv.-Doz. Dr. Kerstin Borchers (firstReferee), Univ.-Prof. Dr. Anja Sterner-Kock (furtherReferee), Univ.-Prof. Dr. Hartmut Weiß (furtherReferee).

Subjects/Keywords: equine herpesvirus; horse diseases; keratoconjunctivitis; immune response; diagnostic techniques; in situ-hybridisation; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Fetsch, A. (2007). EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/10732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Fetsch, Alexandra. “EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors.” 2007. Thesis, Freie Universität Berlin. Accessed August 04, 2020. https://refubium.fu-berlin.de/handle/fub188/10732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Fetsch, Alexandra. “EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors.” 2007. Web. 04 Aug 2020.

Vancouver:

Fetsch A. EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors. [Internet] [Thesis]. Freie Universität Berlin; 2007. [cited 2020 Aug 04]. Available from: https://refubium.fu-berlin.de/handle/fub188/10732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fetsch A. EHV-2 induced equine keratoconjunctivitis Evaluation of the role of immunological mechanisms as well as of viral and bacterial co-factors. [Thesis]. Freie Universität Berlin; 2007. Available from: https://refubium.fu-berlin.de/handle/fub188/10732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.