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You searched for +publisher:"Freie Universität Berlin" +contributor:("PD Dr. S. Hippenstiel"). One record found.

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Freie Universität Berlin

1. Beermann, Wiebke. Histone modifications in the pathogenesis of bacterial infections.

Degree: 2007, Freie Universität Berlin

Histone modifications contribute to the regulation of eukaryotic gene transcription. However, the role of chromatin remodelling in immune responses to intracellular pathogens is poorly understood. Focus of this work lied on clarifying the importance of chromatin remodelling mechanisms as well as signaling pathways in response to infections with Listeria monocytogenes and Chlamydophila pneumoniae as model organism of human endothelial cell infection. L. monocytogenes and C. pneumoniae induced the expression of IL-8 and IFN(gamma), two cytokines that are of great importance for recruitment of inflammatory cells responsible for bacterial elimination. Furthermore, both bacteria induced the secretion of IL-6, TNF(alpha) and G CSF. By using the HDAC inhibitor Trichostatin A (TSA) L. monocytogenes- and C. pneumoniae- induced IL-8 secretion was significantly upregulated while treatment with TSA led to an increased pan-acetylation of histone H4 at the il8 promoter. Moreover, it was demonstrated that endothelial infection by L. monocytogenes and C. pneumoniae induced histone modifikations of histone H3 and H4. The main approach of this work was to analyse two specific histone modifications: phosphorylation/acetylation of histone H3 and pan-acetylation of histone H4. It was shown that both pathogens induced time-dependently phosphorylation/acetylation at serin 10/ lysin 14 of histone H3 and pan acetylation of histone H4, especially at the il8 promoter. In addition, recruitment of RNA polymerase II and NF-(kappa)B/p65 to the il8 promoter could be detected in a time-dependent manner. Infection with L. monocytogenes also demonstrated that the histone acetyl transferase CBP was recruited to the il8 promoter while HDAC1 disappeared. Infectionstudies with L. monocytogenes showed that histone modifications were p38 MAPK and ERK1/2-dependent, especially at the il8 promoter. While recruitment of RNA Pol II, p65 and CBP to the il8 promoter were shown to be regulated by p38-MAPK and ERK1/2, HDAC1 recruitment depended solely on p38-MAPK. On the other hand, L. monocytogenes had no effect on high basal histone acetylation at the ifn(gamma) promoter and p38-MAPK and ERK1/2 were not involved in L. monocytogenes-induced IFN(gamma) expression. L. monocytogenes as well as C. pneumoniae induced IL-8 production in a Rac1-dependend manner in human endothelial cells. Moreover, both bacteria-induced histone modifications were Rac1-dependend and recruitment of RNA Pol II and p65 to the il8 promoter were shown to be reduced after inhibition of Rac1. Finally, simvastatin, an agent known to have beneficial effects on endothelial function by cholesterol reduction and also known to have blocking effects on Rho proteins, reduced C. pneumoniae-induced IL-8 secretion. C. pneumoniae-induced histone modifications were impaired after pre-treatment with simvastatin at protein level and specifically at the il8 promoter. Moreover, recruitment of RNA Polymerase II and NF-(kappa) B/p65 to the il8 promoter was reduced by simvastatin. Taken together, chromatin… Advisors/Committee Members: n (gender), Univ.-Prof. Dr. L.H. Wieler (firstReferee), PD Dr. S. Hippenstiel (furtherReferee), Univ.-Prof. Dr. M.F.G. Schmidt (furtherReferee).

Subjects/Keywords: Bacterial Diseases; Endothelial Cells; Signal Transduction; Histones; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA (6th Edition):

Beermann, W. (2007). Histone modifications in the pathogenesis of bacterial infections. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Beermann, Wiebke. “Histone modifications in the pathogenesis of bacterial infections.” 2007. Thesis, Freie Universität Berlin. Accessed September 19, 2020. https://refubium.fu-berlin.de/handle/fub188/11553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Beermann, Wiebke. “Histone modifications in the pathogenesis of bacterial infections.” 2007. Web. 19 Sep 2020.

Vancouver:

Beermann W. Histone modifications in the pathogenesis of bacterial infections. [Internet] [Thesis]. Freie Universität Berlin; 2007. [cited 2020 Sep 19]. Available from: https://refubium.fu-berlin.de/handle/fub188/11553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Beermann W. Histone modifications in the pathogenesis of bacterial infections. [Thesis]. Freie Universität Berlin; 2007. Available from: https://refubium.fu-berlin.de/handle/fub188/11553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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