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You searched for +publisher:"Freie Universität Berlin" +contributor:("PD Dr. J. Walter"). Showing records 1 – 2 of 2 total matches.

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Freie Universität Berlin

1. Beier, Ilka. Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity.

Degree: 1999, Freie Universität Berlin

In this thesis rec.eqIFN were expressed in several vector systems. The antiviral activity was determined and the proteins displayed. The coding part of the IFN-DNA was cloned in a pro- and eucaryotic vector system (pBK CMV). In the eucaryotic system, biological activity was shown only to a very low extend. In the procaryotic system rec.eqIFN-a and rec.eqIFN-b were detected on protein basis and in the bioactive form. In different bioassays with rec.eqIFN-a the antiviral activity and sensitivity of different cells (ED, EEL, MDBK) and different equine viruses (EHV, VSV, RHINO, BDV) were determined. One of the next vectors (pMal c2) was not suitable for IFN-expression. Furthermore rec.eqIFN-a was cloned in the procaryotic vector pASK IBA2, that uses the Strep-tag-system. Principally it was only possible to get unsoluble protein. After splitting the inclusion bodies and purification of the IFN- protein with the Strep-tag-column the protein could be demonstrated by means of SDS-PAGE. With the third vector (pBAD gIII) an essential enhancement of the antiviral activity of rec.eqIFN-a and -b -proteins by several fold was achieved. After immunization of mice it was possible to produce antibodies against rec.eqIFN-b and to show the proteins in western-blot. After optimizing the expression, interferons can be used for clinical therapy or for diagnostic use. Advisors/Committee Members: n (gender), Univ.-Prof. Dr. Dieter Ebner (firstReferee), PD Dr. J. Walter (furtherReferee).

Subjects/Keywords: interferon; equine; horse; virus; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Beier, I. (1999). Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-13258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Beier, Ilka. “Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity.” 1999. Thesis, Freie Universität Berlin. Accessed August 04, 2020. http://dx.doi.org/10.17169/refubium-13258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Beier, Ilka. “Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity.” 1999. Web. 04 Aug 2020.

Vancouver:

Beier I. Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity. [Internet] [Thesis]. Freie Universität Berlin; 1999. [cited 2020 Aug 04]. Available from: http://dx.doi.org/10.17169/refubium-13258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Beier I. Production and characterization of recombinant equine interferons:expression cloning, demonstration of the proteins and the biological activity. [Thesis]. Freie Universität Berlin; 1999. Available from: http://dx.doi.org/10.17169/refubium-13258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Bach, Barbara. The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model.

Degree: 2008, Freie Universität Berlin

Diaphyseal atrophic non-unions often show biologically disadvantageous conditions for healing. An insufficient vascular supply of the fractured bone as a result of a preceding trauma is considered the main course (Kasperczyk et al., 1996). In such a situation, bone regeneration cannot be achieved without surgical intervention (Gebauer et al., 2005). The current Ā»golden standardĀ« for treatment is to provide a stabilizing osteosynthesis in combination with transplantation of autologous/allogen spongiosa (Braun et al., 1996). However, this kind of therapy does not always guarantee healing. Beside high socio- economic costs, a failure in bone healing is associated with a prolonged recovery time and therefore patient suffering. Moreover repeated surgical interventions may be a risk factor, especially for medically compromised patients. Therefore, over the past years medical science has focused on minimally invasive methods for treatment of delayed and non-unions. In this context, the use of mesenchymal stem cells (MSCs) is considered to be practically, ethically and immunologically inoffensive (Rauner et al., 2007). Thus MSCs represent a promising approach for minimally invasive therapies to enhance bone regeneration (Izdapanah et al., 2005). The present study analyzed the influence of autologous MSCs predifferentiated into the osteogenic lineage in vitro on bone healing. The aim of this study was to examine the therapeutic potential of locally transplanted autologous osteoprogenitor cells (OPCs) in a biologically unreactive fracture situation. Therefore, 32 male adult Sprague DawleyTM rats were randomly devided into two groups, each 16 animals (experimental group, control group). All animals received a tibial bone marrow aspiration. Isolated MSCs were cultured in vitro and predifferentiated into the osteogenic lineage. In all animals, a standardized midshaft osteotomy of the femur diaphysis was performed and stabilized with a monolateral external fixator. An atrophic non-union was induced by thermal destruction of the periosteum 2 mm proximally and distally to the osteotomy gap and bone marrow removal. Two days after surgery, the experimental group received a percutaneous injection of approximately 2 x 106 OPCs/0,1ml expansion medium into the osteotomy gap, whilst the control group was treated with an equivalent volume of pure expansion medium. X-rays were taken directly postoperatively as well as at one and two weeks after surgery. After two weeks, the femora of all animals were explanted and processed for histological/histomorphometrical and immunohistochemical analysis. Radiological and histological examination demonstrated a delay in bone healing. By histomorphometrical analysis, a significantly higher area of the periosteal callus and a higher fraction of cartilage in the periosteal callus at the medial side was found in the experimental compared to the control group. However, there was no difference in the fraction of (mineralized) bone and connective tissue between both groups. The present study demonstrates that… Advisors/Committee Members: n (gender), PD Dr. A. Sterner-Kock, PhD (firstReferee), Univ.-Prof. Dr.-Ing. G.N. Duda (furtherReferee), PD Dr. J. Walter (furtherReferee).

Subjects/Keywords: rats; animal models; fracture healing; postoperative complications; pseudarthrosis; embryonic stem cells; mesenchymal stem cells [MeSH]; cell differentiation; osteogenesis; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bach, B. (2008). The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-6937

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bach, Barbara. “The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model.” 2008. Thesis, Freie Universität Berlin. Accessed August 04, 2020. http://dx.doi.org/10.17169/refubium-6937.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bach, Barbara. “The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model.” 2008. Web. 04 Aug 2020.

Vancouver:

Bach B. The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2020 Aug 04]. Available from: http://dx.doi.org/10.17169/refubium-6937.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bach B. The impact of autologeous mesenchymal stem cells predifferentiated into the osteogenic lineage on bone healing in a rodent atrophic non-union model. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-6937

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.