+publisher:"Florida International University" +contributor:("David Becker")

Florida International University

1. Zhou, Jing. Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation.

Degree: MS, Chemistry, 2013, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1024 ; 10.25148/etd.FI13120903 ; FI13120903

► DNA methylation and demethylation are involved in regulation of gene expression. CpG clusters have been identified as hotspots of oxidative damages and mutagenesis. DNA… (more)

Subjects/Keywords: DNA methylation; DNA demethylation; Base Excision Repair; DNA damage; Biochemistry, Biophysics, and Structural Biology; Genetics and Genomics

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APA (6^th Edition):

Zhou, J. (2013). Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1024 ; 10.25148/etd.FI13120903 ; FI13120903

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Jing. “Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation.” 2013. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/1024 ; 10.25148/etd.FI13120903 ; FI13120903.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Jing. “Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation.” 2013. Web. 15 Apr 2021.

Vancouver:

Zhou J. Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation. [Internet] [Thesis]. Florida International University; 2013. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/1024 ; 10.25148/etd.FI13120903 ; FI13120903.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou J. Roles of DNA Base Excision Repair in Maintaining the Integrity of DNA Methylation. [Thesis]. Florida International University; 2013. Available from: https://digitalcommons.fiu.edu/etd/1024 ; 10.25148/etd.FI13120903 ; FI13120903

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

2. Perez, Gloria Patricia. Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones.

Degree: MS, Chemistry, 2016, Florida International University

URL: https://digitalcommons.fiu.edu/etd/3052 ; 10.25148/etd.FIDC001182 ; FIDC001182

► Mitochondrial dysfunction and oxidative stress have been linked with many pathological conditions. Studies suggest that antioxidants able to accumulate in the inner regions of… (more)

Subjects/Keywords: Hydroxylamine; Organic Chemistry

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APA (6^th Edition):

Perez, G. P. (2016). Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/3052 ; 10.25148/etd.FIDC001182 ; FIDC001182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Perez, Gloria Patricia. “Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones.” 2016. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/3052 ; 10.25148/etd.FIDC001182 ; FIDC001182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Perez, Gloria Patricia. “Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones.” 2016. Web. 15 Apr 2021.

Vancouver:

Perez GP. Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones. [Internet] [Thesis]. Florida International University; 2016. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/3052 ; 10.25148/etd.FIDC001182 ; FIDC001182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Perez GP. Synthetic Studies Toward a Novel Hydroxylamine of Potential Utility in the Preparation of Mitochondriotropic Nitrones. [Thesis]. Florida International University; 2016. Available from: https://digitalcommons.fiu.edu/etd/3052 ; 10.25148/etd.FIDC001182 ; FIDC001182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

3. Tamrakar, Relina. Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection.

Degree: PhD, Chemistry, 2011, Florida International University

URL: https://digitalcommons.fiu.edu/etd/530 ; 10.25148/etd.FI11120906 ; FI11120906

► The superoxide radical is considered to play important roles in physiological processes as well as in the genesis of diverse cytotoxic conditions such as… (more)

▼ The superoxide radical is considered to play important roles in physiological processes as well as in the genesis of diverse cytotoxic conditions such as cancer, various cardiovascular disorders and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson’s disease (PD) and Alzheimer’s disease (AD). The detection and quantification of superoxide within cells is of critical importance to understand biological roles of superoxide and to develop preventive strategies against free radical-mediated diseases. Cyclic nitrone spin traps such as DMPO, EMPO, DEPMPO, BMPO and their derivatives have been widely used in conjunction with ESR spectroscopy to detect cellular superoxide with some success. However, the formation of unstable superoxide adducts from the reaction of cyclic nitrones with superoxide is a stumbling block in detecting superoxide by using electron spin resonance (ESR). A chemiluminescent probe, lucigenin, and fluorogenic probes, hydroethidium and MitoSox, are the other frequently used methods in detecting superoxide. However, luceginen undergoes redox-cycling producing superoxide by itself, and hydroethidium and MitoSox react with other oxidants apart from superoxide forming red fluorescent products contributing to artefacts in these assays. Hence, both methods were deemed to be inappropriate for superoxide detection. In this study, an effective approach, a selective mechanism-based colorimetric detection of superoxide anion has been developed by using silylated azulenyl nitrones spin traps. Since a nitrone moiety and an adjacent silyl group react readily with radicals and oxygen anions respectively, such nitrones can trap superoxide efficiently because superoxide is both a radical and an oxygen anion. Moreover, the synthesized nitrone is designed to be triggered solely by superoxide and not by other commonly observed oxygen radicals such as hydroxyl radical, alkoxyl radicals and peroxyl radical. In vitro studies have shown that these synthesized silylated azylenyl nitrones and the mitochondrial-targeted guanylhydrazone analog can trap superoxide efficiently yielding UV-vis identifiable and even potentially fluorescence-detectable orange products. Therefore, the chromotropic detection of superoxide using these nitrones can be a promising method in contrast to other available methods. Advisors/Committee Members: David Becker, Stanislaw Wnuk, Yong Cai, Richard Bone, Cyril Parkanyi.

Subjects/Keywords: azulenylsilane nitrones; superoxide detection; diagnostic tools

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APA (6^th Edition):

Tamrakar, R. (2011). Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/530 ; 10.25148/etd.FI11120906 ; FI11120906

Chicago Manual of Style (16^th Edition):

Tamrakar, Relina. “Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection.” 2011. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/530 ; 10.25148/etd.FI11120906 ; FI11120906.

MLA Handbook (7^th Edition):

Tamrakar, Relina. “Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection.” 2011. Web. 15 Apr 2021.

Vancouver:

Tamrakar R. Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection. [Internet] [Doctoral dissertation]. Florida International University; 2011. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/530 ; 10.25148/etd.FI11120906 ; FI11120906.

Council of Science Editors:

Tamrakar R. Synthesis of Azulenylsilane Nitrones as Diagnostic Tools for Superoxide Detection. [Doctoral Dissertation]. Florida International University; 2011. Available from: https://digitalcommons.fiu.edu/etd/530 ; 10.25148/etd.FI11120906 ; FI11120906

Florida International University

4. Caraballo, Norma Iris. Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues.

Degree: PhD, Chemistry, 2014, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1391 ; 10.25148/etd.FI14040888 ; FI14040888

► The manner in which remains decompose has been and is currently being researched around the world, yet little is still known about the generated… (more)

▼ The manner in which remains decompose has been and is currently being researched around the world, yet little is still known about the generated scent of death. In fact, it was not until the Casey Anthony trial that research on the odor released from decomposing remains, and the compounds that it is comprised of, was brought to light. The Anthony trial marked the first admission of human decomposition odor as forensic evidence into the court of law; however, it was not “ready for prime time” as the scientific research on the scent of death is still in its infancy. This research employed the use of solid-phase microextraction (SPME) with gas chromatography-mass spectrometry (GC-MS) to identify the volatile organic compounds (VOCs) released from decomposing remains and to assess the impact that different environmental conditions had on the scent of death. Using human cadaver analogues, it was discovered that the environment in which the remains were exposed to dramatically affected the odors released by either modifying the compounds that it was comprised of or by enhancing/hindering the amount that was liberated. In addition, the VOCs released during the different stages of the decomposition process for both human remains and analogues were evaluated. Statistical analysis showed correlations between the stage of decay and the VOCs generated, such that each phase of decomposition was distinguishable based upon the type and abundance of compounds that comprised the odor. This study has provided new insight into the scent of death and the factors that can dramatically affect it, specifically, frozen, aquatic, and soil environments. Moreover, the results revealed that different stages of decomposition were distinguishable based upon the type and total mass of each compound present. Thus, based upon these findings, it is suggested that the training aids that are employed for human remains detection (HRD) canines should 1) be characteristic of remains that have undergone decomposition in different environmental settings, and 2) represent each stage of decay, to ensure that the HRD canines have been trained to the various odors that they are likely to encounter in an operational situation. Advisors/Committee Members: Kenneth G. Furton, José Almirall, David Becker, Yong Cai, DeEtta Mills.

Subjects/Keywords: decomposition odor; human remains; scent of death; human remains detection canines; cadaver dogs; human cadavers; forensics; Analytical Chemistry; Chemistry; Forensic Science and Technology

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APA (6^th Edition):

Caraballo, N. I. (2014). Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1391 ; 10.25148/etd.FI14040888 ; FI14040888

Chicago Manual of Style (16^th Edition):

Caraballo, Norma Iris. “Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues.” 2014. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/1391 ; 10.25148/etd.FI14040888 ; FI14040888.

MLA Handbook (7^th Edition):

Caraballo, Norma Iris. “Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues.” 2014. Web. 15 Apr 2021.

Vancouver:

Caraballo NI. Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues. [Internet] [Doctoral dissertation]. Florida International University; 2014. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/1391 ; 10.25148/etd.FI14040888 ; FI14040888.

Council of Science Editors:

Caraballo NI. Identification of Characteristic Volatile Organic Compounds Released during the Decomposition Process of Human Remains and Analogues. [Doctoral Dissertation]. Florida International University; 2014. Available from: https://digitalcommons.fiu.edu/etd/1391 ; 10.25148/etd.FI14040888 ; FI14040888

Florida International University

5. Sandhaus, Shayna. Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads.

Degree: PhD, Chemistry, 2017, Florida International University

URL: https://digitalcommons.fiu.edu/etd/3561 ; 10.25148/etd.FIDC004005 ; FIDC004005

► Multi-drug resistance in bacterial pathogens has become a global health crisis. Each year, millions of people worldwide are infected with bacterial strains that are… (more)

▼ Multi-drug resistance in bacterial pathogens has become a global health crisis. Each year, millions of people worldwide are infected with bacterial strains that are resistant to currently available antibiotics. Diseases such as tuberculosis, pneumonia, and gonorrhea have become increasingly more difficult to treat. It is essential that novel drugs and cellular targets be identified in order to treat this resistance. Bacterial topoisomerase IA is a novel drug target that is essential for cellular growth. As it has never been targeted by existing antibiotics, it is an attractive target. Topoisomerase IA is responsible for relieving torsional strain on DNA by relaxing supercoiled DNA following processes such as replication and transcription. The aim of this study is to find novel compounds that can be developed as leads for antibiotics targeting bacterial type IA topoisomerase. Various approaches were used in order to screen thousands of compounds against bacterial type IA topoisomerases, including mixture-based screening and virtual screening. In the mixture-based screen, scaffold mixtures were tested against the M. tuberculosis topoisomerase I enzyme and subsequently optimized for maximum potency and selectivity. The optimized compounds were effective at inhibiting the enzyme at low micromolar concentrations, as well as killing the tuberculosis bacteria. In a virtual screen, libraries with hundreds of thousands of compounds were screened against the E. coli and M. tuberculosis topoisomerase I crystal structures in order to find new classes of drugs. The top hits were effective at inhibiting the enzymes, as well as preventing the growth of M. smegmatis cells in the presence of efflux pump inhibitors. Organometallic complexes containing Cu(II) or Co(III) were tested as well against various topoisomerases in order to determine their selectivity. We discovered a poison for human type II topoisomerase that has utility as an anticancer agent, as it killed even very resistant cell lines of breast and colon cancer. The Co(III) complexes were found to inhibit the bacterial topoisomerase I very selectively over other topoisomerases. The various methods of drug discovery utilized here have been successful at identifying new classes of compounds that may be further developed into antibiotic drugs that specifically target bacterial type IA topoisomerases. Advisors/Committee Members: Yuk-Ching Tse-Dinh, David Becker, Niclas Engene, Fenfei Leng, Yuan Liu.

Subjects/Keywords: antibiotic resistance; topoisomerase; drug discovery; Biochemistry, Biophysics, and Structural Biology; Chemistry

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APA (6^th Edition):

Sandhaus, S. (2017). Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/3561 ; 10.25148/etd.FIDC004005 ; FIDC004005

Chicago Manual of Style (16^th Edition):

Sandhaus, Shayna. “Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads.” 2017. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/3561 ; 10.25148/etd.FIDC004005 ; FIDC004005.

MLA Handbook (7^th Edition):

Sandhaus, Shayna. “Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads.” 2017. Web. 15 Apr 2021.

Vancouver:

Sandhaus S. Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads. [Internet] [Doctoral dissertation]. Florida International University; 2017. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/3561 ; 10.25148/etd.FIDC004005 ; FIDC004005.

Council of Science Editors:

Sandhaus S. Drug Candidate Discovery: Targeting Bacterial Topoisomerase I Enzymes for Novel Antibiotic Leads. [Doctoral Dissertation]. Florida International University; 2017. Available from: https://digitalcommons.fiu.edu/etd/3561 ; 10.25148/etd.FIDC004005 ; FIDC004005

Florida International University

6. Liang, Yong. Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides.

Degree: PhD, Chemistry, 2014, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1591 ; 10.25148/etd.FI14110716 ; FI14110716

► The antiviral or anticancer activities of C-5 modified pyrimidine nucleoside analogues validate the need for the development of their syntheses. In the first half… (more)

▼ The antiviral or anticancer activities of C-5 modified pyrimidine nucleoside analogues validate the need for the development of their syntheses. In the first half of this dissertation, I explore the Pd-catalyzed cross-coupling reaction of allylphenylgermanes with aryl halides in the presence of SbF₅/TBAF to give various biaryls by transferring multiple phenyl groups, which has also been applied to the 5-halo pyrimidine nucleosides for the synthesis of 5-aryl derivatives. To avoid the use of organometallic reagents, I developed Pd-catalyzed direct arylation of 5-halo pyrimidine nucleosides. It was discovered that 5-aryl pyrimidine nucleosides could be synthesized by Pd-catalyzed direct arylation of N³-free 5-halo uracil and uracil nucleosides with simple arenes or heteroaromatics in the presence of TBAF within 1 h. Both N³-protected and N³-free uracil and uracil nucleosides could undergo base-promoted Pd-catalyzed direct arylation, but only with electron rich heteroaromatics. In the second half of this dissertation, 5-acetylenic uracil and uracil nucleosides have been employed to investigate the hydrogermylation, hydrosulfonylation as well as hydroazidation for the synthesis of various functionalized 5-vinyl pyrimidine nucleosides. Hydrogermylation of 5-alkynyl uracil analogues with trialkylgermane or tris(trimethylsilyl)germane hydride gave the corresponding vinyl trialkylgermane, or tris(trimethylsilyl)germane uracil derivatives. During the hydrogermylation with triphenylgermane, besides the vinyl triphenylgermane uracil derivatives, 5-[2-(triphenylgermyl)acetyl]uracil was also isolated and characterized and the origin of the acetyl oxygen was clarified. Tris(trimethylsilyl)germane uracil derivatives were coupled to aryl halides but with decent yield. Iron-mediated regio- and stereoselective hydrosulfonylation of the 5-ethynyl pyrimidine analogues with sulfonyl chloride or sulfonyl hydrazine to give 5-(1-halo-2-tosyl)vinyluracil nucleoside derivatives has been developed. Nucleophilic substitution of the 5-(β-halovinyl)sulfonyl nucleosides with various nucleophiles have been performed to give highly functionalized 5-vinyl pyrimidine nucleosides via the addition-elimination mechanism. The 5-(β-keto)sulfonyluracil derivative has also been synthesized via the aerobic difunctionalization of 5-ethynyluracil analogue with sulfinic acid in the presence of catalytic amount of pyridine. Silver catalyzed hydroazidation of protected 2'-deoxy-5-ethynyluridine with TMSN₃ in the presence of catalytic amount of water to give 5-(α-azidovinyl)uracil nucleoside derivatives was developed. Strain promoted Click reaction of the 5-(α-azidovinyl)uracil with cyclooctyne provide the corresponding fully conjugated triazole product. Advisors/Committee Members: Stanislaw F. Wnuk, Kevin O'Shea, David Becker, Piero Gardinali, M. Alejandro Barbieri.

Subjects/Keywords: Nucleoside; Pd-catalyzed Cross-coupling; Palladium; Click Chemistry; Hydrogermylation; C-H activation; Direct Arylation; Organic Chemistry

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APA (6^th Edition):

Liang, Y. (2014). Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1591 ; 10.25148/etd.FI14110716 ; FI14110716

Chicago Manual of Style (16^th Edition):

Liang, Yong. “Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides.” 2014. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/1591 ; 10.25148/etd.FI14110716 ; FI14110716.

MLA Handbook (7^th Edition):

Liang, Yong. “Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides.” 2014. Web. 15 Apr 2021.

Vancouver:

Liang Y. Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides. [Internet] [Doctoral dissertation]. Florida International University; 2014. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/1591 ; 10.25148/etd.FI14110716 ; FI14110716.

Council of Science Editors:

Liang Y. Novel Approaches for the Synthesis of C-5 Modified Pyrimidine Nucleosides. [Doctoral Dissertation]. Florida International University; 2014. Available from: https://digitalcommons.fiu.edu/etd/1591 ; 10.25148/etd.FI14110716 ; FI14110716

7. Corzo, Ruthmara. Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence.

Degree: PhD, Chemistry, 2018, Florida International University

URL: https://digitalcommons.fiu.edu/etd/3814 ; 10.25148/etd.FIDC006819 ; FIDC006819

► Improvements in printing technology have exacerbated the problem of document counterfeiting, prompting the need for analytical techniques that better characterize inks for forensic analysis.… (more)

▼ Improvements in printing technology have exacerbated the problem of document counterfeiting, prompting the need for analytical techniques that better characterize inks for forensic analysis. In this study, 319 printing inks (toner, inkjet, offset, and intaglio) were analyzed directly on the paper substrate using Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS) and Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS). As anticipated, the high sensitivity of LA-ICP-MS resulted in excellent discrimination (> 99%) between ink samples originating from different sources. Moreover, LA-ICP-MS provided ≥ 90% correct association for ink samples originating from the same source. SEM-EDS resulted in good discrimination for toner and intaglio inks (> 97%) and excellent correct association (100%) for all four ink types. However, the technique showed limited utility for the discrimination of inkjet and offset inks. A searchable ink database, the Forensic Ink Analysis and Comparison System (FIACS), was developed in order to provide a tool that allows the analyst to compare a questioned ink sample to a reference population. The FIACS database provided a correct classification rate of 94-100% for LA-ICP-MS and 67-100% for SEM-EDS. An important consideration in forensic chemistry is the interpretation of the evidence. Typically, a match criterion is used to compare the known and questioned sample. However, this approach suffers from several disadvantages, which can be overcome with an alternative approach: the likelihood ratio (LR). Two LA-ICP-MS glass databases were used to evaluate the performance of the LR: a vehicle windshield database (420 samples) and a casework database (385 samples). Compared to the match criterion, the likelihood ratio led to improved false exclusion rates (< 1.5%) and similar false inclusion rates (< 1.0%). In addition, the LR limited the magnitude of the misleading evidence, providing only weak support for the incorrect proposition. The likelihood ratio was also tested through an inter-laboratory study including 10 LA-ICP-MS participants. Good correct association rates (94-100%) were obtained for same-source samples for all three inter-laboratory exercises. Moreover, the LR showed a strong support for an association. Finally, all different-source samples were correctly excluded with the LR, resulting in no false inclusions. Advisors/Committee Members: Jose Almirall, David Becker, Yong Cai, Anthony DeCaprio, Werner Boeglin.

Subjects/Keywords: Forensic; Ink; Glass; Likelihood Ratio; SEM-EDS; LA-ICP-MS; LIBS; Database; Analytical Chemistry

…Forensic Ink Analysis and Comparison System FIU Florida International University FRSD Fixed…

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APA (6^th Edition):

Corzo, R. (2018). Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/3814 ; 10.25148/etd.FIDC006819 ; FIDC006819

Chicago Manual of Style (16^th Edition):

Corzo, Ruthmara. “Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence.” 2018. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/3814 ; 10.25148/etd.FIDC006819 ; FIDC006819.

MLA Handbook (7^th Edition):

Corzo, Ruthmara. “Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence.” 2018. Web. 15 Apr 2021.

Vancouver:

Corzo R. Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence. [Internet] [Doctoral dissertation]. Florida International University; 2018. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/3814 ; 10.25148/etd.FIDC006819 ; FIDC006819.

Council of Science Editors:

Corzo R. Elemental Characterization of Printing Inks and Strengthening the Evaluation of Forensic Glass Evidence. [Doctoral Dissertation]. Florida International University; 2018. Available from: https://digitalcommons.fiu.edu/etd/3814 ; 10.25148/etd.FIDC006819 ; FIDC006819

8. sun, pengfei. Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis.

Degree: PhD, Chemistry, 2015, Florida International University

URL: https://digitalcommons.fiu.edu/etd/2066 ; 10.25148/etd.FIDC000133 ; FIDC000133

► Polyether compounds are a subgroup of natural products with regular occurrence of multiple C-O-C motifs. The biosynthetic origin of polycylic polethers has been studied… (more)

▼ Polyether compounds are a subgroup of natural products with regular occurrence of multiple C-O-C motifs. The biosynthetic origin of polycylic polethers has been studied and the majority of them are derived from polyketide or terpene pathways. Normally, the polycyclic polyethers can be divided into two groups based on their structural features: the first group features multiple rings that are interconnected by carbon-carbon single bond, which are produced by a biosynthetic cascade of exo epoxide-opening reactions; the other group has multiple fused cyclic ethers and are formed by an cascade of endo epoxide-opening reactions. Karenia brevis (K. brevis) is known as principle harmful bloom (HAB) organism of the Gulf of Mexico which can cause red tides. Brevetoxins (PbTx) are a suit of cyclic polyether ladder compounds produced by K. brevis. Brevetoxins are neurotoxins that can bind to voltage-gated sodium channels in nerve and muscle cells, resulting in disruption of normal neurological processes causing the human illness which is clinically described as neurotoxic shellfish poisoning (NSP). Inspired by Cane-Celmer-Wesley’s proposal regarding monensin biosynthesis, Nakanishi and Shimizu proposed a biosynthetic pathway for brevetoxin which suggests that PKS-mediated synthesis of the polyene is followed by epoxidation to afford a polyepoxide which then undergoes an epoxide-opening cascade, catalyzed by an epoxide hydrolase (EH). To find evidence to support the hypothesis that an epoxide hydrolase from polyether ladder producing dinoflagellates will catalyze the construction of the polyether ladder framework from polyepoxide substrates, and to study the role of epoxide hydrolase in the biosynthesis of polyether ladder compounds, it is necessary to identify and produce one or more epoxide hydrolase from dinoflagellates. The methods to detect epoxide hydrolase activity in K. brevis and different techniques to obtain epoxide hydrolases from K. brevis are discussed. A microsomal EH identified from a K. brevis EST library was cloned and expressed. The characterization of this EH, including substrate selectivity and enantioselectivity as well as its potential to catalyze the critical ento-tet cyclization epoxy alcohol, is discussed. Advisors/Committee Members: Kathleen S. Rein, David Becker, Watson Lees, Lidia Kos, Xiaotang Wang.

Subjects/Keywords: Karenia brevis; dinoflagellate; brevetoxin; epoxide hydrolase; Biochemistry; Molecular Biology

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APA (6^th Edition):

sun, p. (2015). Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/2066 ; 10.25148/etd.FIDC000133 ; FIDC000133

Chicago Manual of Style (16^th Edition):

sun, pengfei. “Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis.” 2015. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/2066 ; 10.25148/etd.FIDC000133 ; FIDC000133.

MLA Handbook (7^th Edition):

sun, pengfei. “Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis.” 2015. Web. 15 Apr 2021.

Vancouver:

sun p. Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis. [Internet] [Doctoral dissertation]. Florida International University; 2015. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/2066 ; 10.25148/etd.FIDC000133 ; FIDC000133.

Council of Science Editors:

sun p. Characterization of an Epoxide Hydrolase from the Florida Red Tide Dinoflagellate, Karenia brevis. [Doctoral Dissertation]. Florida International University; 2015. Available from: https://digitalcommons.fiu.edu/etd/2066 ; 10.25148/etd.FIDC000133 ; FIDC000133

9. Wang, Wentian. Asymmetric Syntheses of Analogs of Kainic Acid.

Degree: PhD, Chemistry, 2012, Florida International University

URL: https://digitalcommons.fiu.edu/etd/756 ; 10.25148/etd.FI12113007 ; FI12113007

► Kainic acid has been used for nearly 50 years as a tool in neuroscience due to its pronounced neuroexcitatory properties. However, the significant price… (more)

▼ Kainic acid has been used for nearly 50 years as a tool in neuroscience due to its pronounced neuroexcitatory properties. However, the significant price increase of kainic acid resulting from the disruption in the supply from its natural source, the alga Digenea Simplex, as well as inefficient synthesis of kainic acid, call for the exploration of functional mimics of kainic acid that can be synthesized in a simpler way. Aza kainoids analog could be one of them. The unsubstituted aza analog of kainoids has demonstrates its ability as an ionotropic glutamate receptor agonist and showed affinity in the chloride dependent glutamate (GluCl) binding site. This opened a question of the importance of the presence of one nitrogen or both nitrogens in the aza kainoid analogs for binding to glutamate receptors. Therefore, two different pyrrolidine analogs of kainic acid, trans-4-(carboxymethyl)pyrrolidine-3-carboxylic acid and trans-2-carboxy-3-pyrrolidineacetic acid, were synthesized through multi-step sequences. The lack of the affinity of both pyrrolidine analogs in GluCl binding site indicated that both nitrogens in aza kainoid analogs are involved in hydrogen bonding with receptors, significantly enhancing their affinity in GluCl binding site. Another potential functional mimic of kainic acid is isoxazolidine analogs of kainoids whose skeleton can be constituted directly via a 1, 3 dipolar cycloaddition as the key step. The difficulty in synthesizing N-unsubstituted isoxazolidines when applying such common protecting groups as alkyl, phenyl and benzyl groups, and the requirement of a desired enantioselectivity due to the three chiral ceneters in kainic acid, pose great challenges. Hence, several different protected nitrones were studied to establish that diphenylmethine nitrone may be a good candidate as the dipole in that the generated isoxazolidines can be deprotected in mild conditions with high yields. Our investigations also indicated that the exo/endo selectivity of the 1, 3 dipolar cycloaddition can be controlled by Lewis acids, and that the application of a directing group in dipolarophiles can accomplish a satisfied enantioselectivity. Those results demonstrated the synthesis of isoxazoldines analogs of kainic acid is very promising. Advisors/Committee Members: Kathleen S. Rein, David Becker, Kevin O'Shea, Philip Stoddard, Waston Lees.

Subjects/Keywords: Kainic Acid; Pyrrolidine; Dipolar cycloaddition; Asymmetric; Exo/Endo; Isoxazolidine; Mosher method

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APA (6^th Edition):

Wang, W. (2012). Asymmetric Syntheses of Analogs of Kainic Acid. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/756 ; 10.25148/etd.FI12113007 ; FI12113007

Chicago Manual of Style (16^th Edition):

Wang, Wentian. “Asymmetric Syntheses of Analogs of Kainic Acid.” 2012. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/756 ; 10.25148/etd.FI12113007 ; FI12113007.

MLA Handbook (7^th Edition):

Wang, Wentian. “Asymmetric Syntheses of Analogs of Kainic Acid.” 2012. Web. 15 Apr 2021.

Vancouver:

Wang W. Asymmetric Syntheses of Analogs of Kainic Acid. [Internet] [Doctoral dissertation]. Florida International University; 2012. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/756 ; 10.25148/etd.FI12113007 ; FI12113007.

Council of Science Editors:

Wang W. Asymmetric Syntheses of Analogs of Kainic Acid. [Doctoral Dissertation]. Florida International University; 2012. Available from: https://digitalcommons.fiu.edu/etd/756 ; 10.25148/etd.FI12113007 ; FI12113007

10. Wen, Zhiwei. Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities.

Degree: PhD, Chemistry, 2018, Florida International University

URL: https://digitalcommons.fiu.edu/etd/3789 ; 10.25148/etd.FIDC006844 ; FIDC006844

► Two classes of C5 azido-modified pyrimidine nucleosides were synthesized and explored as radiosensitizers. The 5-azidomethyl-2'-deoxyuridine (AmdU) was prepared from thymidine and converted to its… (more)

▼ Two classes of C5 azido-modified pyrimidine nucleosides were synthesized and explored as radiosensitizers. The 5-azidomethyl-2'-deoxyuridine (AmdU) was prepared from thymidine and converted to its cytosine counterpart (AmdC). The 5-(1-azidovinyl) modified 2'-deoxyuridine (AvdU) and 2'-deoxycytidine (AvdC) were prepared employing regioselective Ag-catalyzed hydroazidation of 5-ethynyl pyrimidine substrates with TMSN₃. AmdU and AmdC were converted to 5'-triphosphates AmdUTP and AmdCTP, and incorporated into DNA-fragments via polymerase-catalyzed reaction during DNA replication and base excision repair. Radiation-mediated prehydrated electrons formed in homogeneous aqueous glassy (7.5 M LiCl) systems in the absence of oxygen at 77 K led to site-specific formation of π-type aminyl radicals (RNH•) from AmdU, AmdC, AvdU, and AvdC. The ESR spectral studies and DFT calculations showed RNH• undergo facile conversion to thermodynamically more stable σ-type iminyl radicals, R=N•. For AmdU, conversion of RNH• to R=N• was bimolecular involving α-azidoalkyl radical as intermediate; however, for AvdU, RNH• tautomerized to R=N•. Our work provides the first evidence for the formation of RNH• attached to C5 position of azidopyrimidine nucleoside and its facile conversion to R=N• under reductive environment. These aminyl and iminyl radicals can generate DNA damage via oxidative pathways. The azido-nucleosides were successfully applied as radiosensitizers in EMT6 cancer cells in both hypoxic and normoxic conditions. To explore the generation and reactivity of 2'‑deoxyguanosin-N2-yl radical (dG(N2-H)•) postulated to generate from guanine moiety towards •OH, 2-azido-2'-deoxyinosine (2-N₃dI) was prepared by conversion of 2-amino group in protected dG into 2-azido via diazotization with tert-butyl nitrite followed by displacement with azide and deprotection. The investigation of dG(N2-H)• generated from 2-N₃dI and its subsequent reactions using ESR will be discussed. Cycloaddition between 5-ethynylpyrimidine or 8-ethynylpurine nucleosides and TMSN₃ in the presence of Ag₂CO₃, CuI, or CuSO₄/sodium ascorbate provided N-unsubstituted 1,2,3-triazol-4-yl analogues of the parental DNA bases (i.e. 5-TrzdU, 5‑TrzdC, 8-TrzdA, and 8-TrzdG). These novel triazolyl nucleosides showed excellent fluorescent properties: 8-TrzdA exhibits the highest quantum yield (Φ_F) of 44% while 8‑TrzdG had Φ_F of 9%. The 5-TrzdU and 5-TrzdC showed a large Stokes shift of ~110 nm. The application of these fluorescent nucleosides to cell imaging and DNA modifications will also be discussed. Advisors/Committee Members: Stanislaw F. Wnuk, Kevin O'Shea, Kathleen Rein, David Becker, Anthony J. McGoron.

Subjects/Keywords: azide; aminyl radical; iminyl radical; anticancer; hypoxia; radiosensitizer; triazole; fluorescent; nucleoside analogues; Nucleic Acids, Nucleotides, and Nucleosides; Organic Chemicals

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APA (6^th Edition):

Wen, Z. (2018). Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/3789 ; 10.25148/etd.FIDC006844 ; FIDC006844

Chicago Manual of Style (16^th Edition):

Wen, Zhiwei. “Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities.” 2018. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/3789 ; 10.25148/etd.FIDC006844 ; FIDC006844.

MLA Handbook (7^th Edition):

Wen, Zhiwei. “Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities.” 2018. Web. 15 Apr 2021.

Vancouver:

Wen Z. Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities. [Internet] [Doctoral dissertation]. Florida International University; 2018. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/3789 ; 10.25148/etd.FIDC006844 ; FIDC006844.

Council of Science Editors:

Wen Z. Azido- and Triazolyl-modified Nucleoside/tide Analogues: Chemistry, Fluorescent Properties, and Anticancer Activities. [Doctoral Dissertation]. Florida International University; 2018. Available from: https://digitalcommons.fiu.edu/etd/3789 ; 10.25148/etd.FIDC006844 ; FIDC006844

Florida International University

11. Alvarez, Carlos Andres. Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase.

Degree: MS, Chemistry, 2006, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1261 ; 10.25148/etd.FI14031601 ; FI14031601

► Chloroperoxidase (CPO), a 298-residue glycosylated protein from the fungus Caldariomyces fumago, is probably the most versatile heme enzyme yet discovered. Interest in CPO as… (more)

Subjects/Keywords: Chemistry

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APA (6^th Edition):

Alvarez, C. A. (2006). Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1261 ; 10.25148/etd.FI14031601 ; FI14031601

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alvarez, Carlos Andres. “Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase.” 2006. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/1261 ; 10.25148/etd.FI14031601 ; FI14031601.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alvarez, Carlos Andres. “Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase.” 2006. Web. 15 Apr 2021.

Vancouver:

Alvarez CA. Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase. [Internet] [Thesis]. Florida International University; 2006. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/1261 ; 10.25148/etd.FI14031601 ; FI14031601.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alvarez CA. Docking and simulation study of the enantiospecificity of chiral epoxidation reactions catalyzed by chloroperoxidase. [Thesis]. Florida International University; 2006. Available from: https://digitalcommons.fiu.edu/etd/1261 ; 10.25148/etd.FI14031601 ; FI14031601

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

12. Das, Baishakhi. Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD).

Degree: MS, Chemistry, 2006, Florida International University

URL: https://digitalcommons.fiu.edu/etd/2735 ; 10.25148/etd.FI14062201 ; FI14062201

► A study of the reaction pathways of 1,1-diphenylethylene; methyleneindan; fluorenene; trans,trans-1,4-diphenyl-1,3-butadiene; cis,trans-1,4-diphenyl-1,3- butadiene and cis,cis-1,4-diphenyl-1,3-butadiene with singlet oxygen and the singlet oxygen mimic, 4-alkyl-1,2,4-triazoline-3,5-dione… (more)

Subjects/Keywords: Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Das, B. (2006). Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD). (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/2735 ; 10.25148/etd.FI14062201 ; FI14062201

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Das, Baishakhi. “Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD).” 2006. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/2735 ; 10.25148/etd.FI14062201 ; FI14062201.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Das, Baishakhi. “Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD).” 2006. Web. 15 Apr 2021.

Vancouver:

Das B. Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD). [Internet] [Thesis]. Florida International University; 2006. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/2735 ; 10.25148/etd.FI14062201 ; FI14062201.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Das B. Reactions of 1,1-disubstituted-ethylenes and 1,4-Diphenyl-1,3-Butadienes with singlet oxygen and 4-Alkyl-1,2,4-triazoline-3,5-dione (RTAD). [Thesis]. Florida International University; 2006. Available from: https://digitalcommons.fiu.edu/etd/2735 ; 10.25148/etd.FI14062201 ; FI14062201

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

13. Kolhe, Amolkumar. Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones.

Degree: Chemistry, 2009, Florida International University

URL: https://digitalcommons.fiu.edu/etd/197 ; 10.25148/etd.FI10022526 ; FI10022526

► Free radicals have been implicated in various pathological conditions such as, stroke, aging and ischemic heart disease (IHD), as well as neurodegenerative diseases like… (more)

▼ Free radicals have been implicated in various pathological conditions such as, stroke, aging and ischemic heart disease (IHD), as well as neurodegenerative diseases like Alzheimer’s, Parkinson’s, and Huntington’s disease. The role of antioxidants in protection from the harmful effects of free radicals has long been recognized. Trapping extremely reactive free radicals and eliminating them from circulation has been shown to be effective in animal models. Nitrone-based free radical traps have been extensively explored in biological systems. Examples include nitrones such as PBN, NXY-059, MDL-101,002, DMPO and EMPO. However, these nitrones have extremely high oxidation potentials as compared to natural antioxidants such as Vitamin E (á-tocopherol), and glutathione. Becker et al. (1995) synthesized novel azulenyl nitrones, which were shown to have oxidation potentials much lower than that of any of the previously reported nitrone based spin traps. Another azulenyl nitrone derivative, stilbazulenyl nitrone (STAZN), was shown to have an even lower oxidation potential within the range of natural antioxidants. STAZN, a second generation free radical trap, was found to be markedly superior than the two most studied nitrones, PBN and NXY-059, in animal models of cerebral ischemia and in an in vitro assay of lipid peroxidation. In this study, a third generation azulenyl nitrone was synthesized with an electron donating group on the previously synthesized STAZN derivative with the aim to lower the oxidation potential even more. Pseudoazulenes, because of the presence of an annular heteroatom, have been reported to possess even lower oxidation potential than that of the azulenyl counterpart. Therefore, pseudoazulenyl nitrones were synthesized for the first time by extracting and elaborating valtrate from the roots of Centranthus ruber (Red valerian or Jupiter’s beard). Several pseudoazulenyl nitrones were synthesized by using a facile experimental protocol. The physical and biological properties of these pseudoazulenyl nitrones can be easily modified by simply changing the substituent on the heteroatom. Cyclic voltammetry experiments have shown that these pseudoazulenyl nitrones do indeed have low oxidation potentials. The oxidation potential of these nitrones was lowered even more by preparing derivatives bearing an electron donating group at the 3-position of the five membered ring of the pseudoazulenyl nitrone. Advisors/Committee Members: David Becker, John Landrum, Ophelia Weeks, Stainslaw Wnuk, Watson Lees.

Subjects/Keywords: spin traps; free radicals; nitrones; antioxidants; azulenyl; azlene; pseudoazulene; pseudoazulenyl

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APA (6^th Edition):

Kolhe, A. (2009). Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/197 ; 10.25148/etd.FI10022526 ; FI10022526

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kolhe, Amolkumar. “Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones.” 2009. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/197 ; 10.25148/etd.FI10022526 ; FI10022526.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kolhe, Amolkumar. “Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones.” 2009. Web. 15 Apr 2021.

Vancouver:

Kolhe A. Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones. [Internet] [Thesis]. Florida International University; 2009. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/197 ; 10.25148/etd.FI10022526 ; FI10022526.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kolhe A. Synthetic Studies of Azulenyl and Pseudoazulenyl Nitrones. [Thesis]. Florida International University; 2009. Available from: https://digitalcommons.fiu.edu/etd/197 ; 10.25148/etd.FI10022526 ; FI10022526

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

14. Andrei, Daniela. Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry.

Degree: PhD, Chemistry, 2006, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1287 ; 10.25148/etd.FI14032316 ; FI14032316

► The enzyme S-adenosyl-L-homocysteine (AdoHey) hydrolase effects hydrolytic cleavage of AdoHcy to adenosine (Ado) and L-homocysteine (Hcy). The cellular levels of AdoHcy and Hcy are… (more)

▼ The enzyme S-adenosyl-L-homocysteine (AdoHey) hydrolase effects hydrolytic cleavage of AdoHcy to adenosine (Ado) and L-homocysteine (Hcy). The cellular levels of AdoHcy and Hcy are critical because AdoHcy is a potent feedback inhibitor of crucial transmethylation enzymes. Also, elevated plasma levels of Hcy in humans have been shown to be a risk factor in coronary artery disease. On the basis of the previous finding that AdoHcy hydrolase is able to add the enzyme-sequestered water molecule across the 5',6'-double bond of (halo or dihalohomovinyl)-adenosines causing covalent binding inhibition, we designed and synthesized AdoHcy analogues with the 5',6'-olefin motif incorporated in place of the carbon-5' and sulfur atoms. From the available synthetic methods we chose two independent approaches: the first approach was based on the construction of a new C5'- C6' double bond via metathesis reactions, and the second approach was based on the formation of a new C6'-C7' single bond via Pd-catalyzed cross-couplings. Cross-metathesis of the suitably protected 5'-deoxy-5'-methyleneadenosine with racemic 2-amino-5-hexenoate in the presence of Hoveyda-Grubb's catalyst followed by standard deprotection afforded the desired analogue as 5'E isomer of the inseparable mixture of 9'RIS diastereomers. Metathesis of chiral homoallylglycine [(2S)-amino-5-hexenoate] produced AdoHcy analogue with established stereochemistry E at C5'atom and S at C9' atom. The 5'-bromovinyl analogue was synthesized using the brominationdehydrobromination strategy with pyridinium tribromide and DBU. Since literature reports on the Pd-catalyzed monoalkylation of dihaloalkenes (Csp2-Csp3 coupling) were scarce, we were prompted to undertake model studies on Pdcatalyzed coupling between vinyl dihalides and alkyl organometallics. The 1-fluoro-1- haloalkenes were found to undergo Negishi couplings with alkylzinc bromides to give multisubstituted fluoroalkenes. The alkylation was trans-selective affording pure Zfluoroalkenes. The highest yields were obtained with PdCl 2(dppb) catalyst, but the best stereochemical outcome was obtained with less reactive Pd(PPh3)4 . Couplings of 1,1- dichloro-and 1,1-dibromoalkenes with organozinc reagents resulted in the formation of monocoupled 1-halovinyl product. Advisors/Committee Members: Stanislaw Whuk, David Becker, Piero Gardinali, Kevin O'Shea, Richard Schoephoerster.

Subjects/Keywords: Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrei, D. (2006). Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1287 ; 10.25148/etd.FI14032316 ; FI14032316

Chicago Manual of Style (16^th Edition):

Andrei, Daniela. “Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry.” 2006. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/1287 ; 10.25148/etd.FI14032316 ; FI14032316.

MLA Handbook (7^th Edition):

Andrei, Daniela. “Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry.” 2006. Web. 15 Apr 2021.

Vancouver:

Andrei D. Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry. [Internet] [Doctoral dissertation]. Florida International University; 2006. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/1287 ; 10.25148/etd.FI14032316 ; FI14032316.

Council of Science Editors:

Andrei D. Synthesis of multisubstituted halo-olefins via Pd-catalyzed cross-coupling reactions : applications in nucleoside chemistry. [Doctoral Dissertation]. Florida International University; 2006. Available from: https://digitalcommons.fiu.edu/etd/1287 ; 10.25148/etd.FI14032316 ; FI14032316

Florida International University

15. Chen, Yin. A study of cartenoids uptake in human subjects and their metabolites.

Degree: MS, Chemistry, 1999, Florida International University

URL: https://digitalcommons.fiu.edu/etd/2148 ; 10.25148/etd.FI14060180 ; FI14060180

► In this study, a metabolite of lutein was tentatively shown to result from oxidation of lutein by performing a reduction reaction. The result is… (more)

Subjects/Keywords: Chemistry

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APA (6^th Edition):

Chen, Y. (1999). A study of cartenoids uptake in human subjects and their metabolites. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/2148 ; 10.25148/etd.FI14060180 ; FI14060180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Yin. “A study of cartenoids uptake in human subjects and their metabolites.” 1999. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/2148 ; 10.25148/etd.FI14060180 ; FI14060180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Yin. “A study of cartenoids uptake in human subjects and their metabolites.” 1999. Web. 15 Apr 2021.

Vancouver:

Chen Y. A study of cartenoids uptake in human subjects and their metabolites. [Internet] [Thesis]. Florida International University; 1999. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/2148 ; 10.25148/etd.FI14060180 ; FI14060180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen Y. A study of cartenoids uptake in human subjects and their metabolites. [Thesis]. Florida International University; 1999. Available from: https://digitalcommons.fiu.edu/etd/2148 ; 10.25148/etd.FI14060180 ; FI14060180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

16. Companioni, Dania. Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides.

Degree: MS, Chemistry, 2002, Florida International University

URL: https://digitalcommons.fiu.edu/etd/2417 ; 10.25148/etd.FI14060887 ; FI14060887

► Inhibition of the enzyme ribonucleotide reductase is an appealing concept for the rational drug design against rapid proliferation of systems such as viruses and… (more)

Subjects/Keywords: Chemistry

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APA (6^th Edition):

Companioni, D. (2002). Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/2417 ; 10.25148/etd.FI14060887 ; FI14060887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Companioni, Dania. “Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides.” 2002. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/2417 ; 10.25148/etd.FI14060887 ; FI14060887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Companioni, Dania. “Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides.” 2002. Web. 15 Apr 2021.

Vancouver:

Companioni D. Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides. [Internet] [Thesis]. Florida International University; 2002. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/2417 ; 10.25148/etd.FI14060887 ; FI14060887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Companioni D. Targeting of ribonucleotide reductase with 2'-Disulfide analogs of adenosine. Electronic versus steric effects in radical deoxygenation of fluorine-containing nucleosides. [Thesis]. Florida International University; 2002. Available from: https://digitalcommons.fiu.edu/etd/2417 ; 10.25148/etd.FI14060887 ; FI14060887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

17. Pitteloud, Jean-Philippe. New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues.

Degree: Chemistry, 2010, Florida International University

URL: https://digitalcommons.fiu.edu/etd/170 ; 10.25148/etd.FI10041621 ; FI10041621

► The diverse biological properties exhibited by uridine analogues modified at carbon-5 of the uracil base have attracted special interest to the development of efficient… (more)

▼ The diverse biological properties exhibited by uridine analogues modified at carbon-5 of the uracil base have attracted special interest to the development of efficient methodologies for their synthesis. This study aimed to evaluate the possible application of vinyl tris(trimethylsilyl)germanes in the synthesis of conjugated 5-modified uridine analogues via Pd-catalyzed cross-coupling reactions. The stereoselective synthesis of 5-[(2-tris(trimethylsilyl)germyl)ethenyl]uridine derivatives was achieved by the radical-mediated hydrogermylation of the protected 5-alkynyluridine precursors with tris(trimethylsilyl)germane [(TMS)3GeH]. The hydrogermylation with Ph3GeH afforded in addition to the expected 5-vinylgermane, novel 5-(2-triphenylgermyl)acetyl derivatives. Also, the treatment with Me3GeH provided access to 5-vinylgermane uridine analogues with potential biological applications. Since the Pd-catalyzed cross-coupling of organogermanes has received much less attention than the couplings involving organostannanes and organosilanes, we were prompted to develop novel organogermane precursors suitable for transfer of aryl and/or alkenyl groups. The allyl(phenyl)germanes were found to transfer allyl groups to aryl iodides in the presence of sodium hydroxide or tetrabutylammonium fluoride (TBAF) via a Heck arylation mechanism. On the other hand, the treatment of allyl(phenyl)germanes with tetracyanoethylene (TCNE) effectively cleaved the Ge-C(allyl) bonds and promoted the transfer of the phenyl groups upon fluoride activation in toluene. It was discovered that the trichlorophenyl,- dichlorodiphenyl,- and chlorotriphenylgermanes undergo Pd-catalyzed cross-couplings with aryl bromides and iodides in the presence of TBAF in toluene with addition of the measured amount of water. One chloride ligand on the Ge center allows efficient activation by fluoride to promote transfer of one, two or three phenyl groups from the organogermane precursors. The methodology shows that organogermanes can render a coupling efficiency comparable to the more established stannane and silane counterparts. Our coupling methodology (TBAF/moist toluene) was also found to promote the transfer of multiple phenyl groups from analogous chloro(phenyl)silanes and stannanes. Advisors/Committee Members: Stanislaw F. Wnuk, David Becker, Kevin O'Shea, Watson Lees, David Lee.

Subjects/Keywords: ORGANOGERMANES; PALLADIUM-CATALYZED; CROSS-COUPLING; TRANSMETALLATION; URIDINE; NUCLEOSIDES

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pitteloud, J. (2010). New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues. (Thesis). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/170 ; 10.25148/etd.FI10041621 ; FI10041621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pitteloud, Jean-Philippe. “New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues.” 2010. Thesis, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/170 ; 10.25148/etd.FI10041621 ; FI10041621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pitteloud, Jean-Philippe. “New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues.” 2010. Web. 15 Apr 2021.

Vancouver:

Pitteloud J. New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues. [Internet] [Thesis]. Florida International University; 2010. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/170 ; 10.25148/etd.FI10041621 ; FI10041621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pitteloud J. New Organogermanium Substrates for Palladium-Catalyzed Cross-Coupling Reactions. Application of Organogermanes towards the Synthesis of Carbon-5 Modified Uridine Analogues. [Thesis]. Florida International University; 2010. Available from: https://digitalcommons.fiu.edu/etd/170 ; 10.25148/etd.FI10041621 ; FI10041621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida International University

18. Gao, Min. Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation.

Degree: PhD, Chemistry, 2007, Florida International University

URL: https://digitalcommons.fiu.edu/etd/3618 ; FI15101500

► Freshwater wetland soils of the Everglades were studied in order to assess present environmental conditions and paleo-environmental changes using organic geochemistry techniques. Organic matter… (more)

▼ Freshwater wetland soils of the Everglades were studied in order to assess present environmental conditions and paleo-environmental changes using organic geochemistry techniques. Organic matter in dominant vegetation, peat and marl soils was characterized by geochemical means. Samples were selected along nutrient and hydrology gradients with the objective to determine the historical sources of organic matter as well as the extent of its preservation. Effective molecular proxies were developed to differentiate the relative input of organic matter from different biological sources to wetland soils. Thus historical vegetation shifts and hydroperiods were reconstructed using those proxies. The data show good correlations with historical water management practices starting at the turn of the century and during the mid 1900’s. Overall, significant shortening of hydroperiods during this period was observed. The soil organic matter (SOM) preservation was assessed through elemental analysis and molecular characterizations of bulk 13C stable isotopes, solid state 13C NMR spectroscopy, UV-Vis spectroscopy, and tetramethyl ammonium hydroxide (TMAH) thermochemolysis-GC/MS. The relationship of the environmental conditions and degradation status of the soil organic matter (SOM) among the sites suggested that both high nutrient levels and long hydroperiod favor organic matter degradation in the soils. This is probably the result of an increase in the microbial activity in the soils which have higher nutrient levels, while longer hydroperiods may enhance physical/chemical degradation processes. The most significant transformations of biomass litter in this environment are controlled by very early physical/chemical processes and once the OM is incorporated into surface soils, the diagenetic change, even over extended periods of time is comparatively minimal, and SOM is relatively well preserved regardless of hydroperiod or nutrient levels. SOM accumulated in peat soils is more prone to continued degradation than the SOM in the marl soils. The latter is presumably stabilized early on through direct air exposure (oxidation) and thus, it is more refractory to further diagenetic transformations such as humification and aromatization reactions. Advisors/Committee Members: Rudolf Jaffé, Michael E. McClain, Yong Cai, David Becker, José R. Almirall.

Subjects/Keywords: Chemistry; Physical Sciences and Mathematics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, M. (2007). Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/3618 ; FI15101500

Chicago Manual of Style (16^th Edition):

Gao, Min. “Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation.” 2007. Doctoral Dissertation, Florida International University. Accessed April 15, 2021. https://digitalcommons.fiu.edu/etd/3618 ; FI15101500.

MLA Handbook (7^th Edition):

Gao, Min. “Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation.” 2007. Web. 15 Apr 2021.

Vancouver:

Gao M. Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation. [Internet] [Doctoral dissertation]. Florida International University; 2007. [cited 2021 Apr 15]. Available from: https://digitalcommons.fiu.edu/etd/3618 ; FI15101500.

Council of Science Editors:

Gao M. Chemical characterization of soil organic matter in an oligotrophic, subtropical, freshwatwer wetland system : sources, diagenesis and preservation. [Doctoral Dissertation]. Florida International University; 2007. Available from: https://digitalcommons.fiu.edu/etd/3618 ; FI15101500

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