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You searched for +publisher:"Eastern Michigan University" +contributor:("Michael Angell, PhD"). Showing records 1 – 2 of 2 total matches.

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Eastern Michigan University

1. Sepehri, Saedeh J. A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus.

Degree: MS, Biology, 2011, Eastern Michigan University

This study was to determine if one or more loci are serving as a master gene for the amplification of LINE, ID, and mys elements, which mobilize via RNA, in Peromyscus maniculatus tissues, by the use of the RT-PCR technique. It was concluded that potentially more than one master gene is responsible for the amplification of LINE and mys in P.maniculatus. RT-PCR products of testes and brain for ID matched that of the BC1 gene, known to be the master gene for ID elements. However, none of the cloned products detected from ovary matched any gene available in the database. Therefore, L1 and ID might not be retrotransposed in the ovary, and BC1 might not be the master gene responsible for the amplification of the ID elements in ovary tissue. The ID in ovary potentially belongs to a more recent family of ID that hasn‘t been previously demonstrated in P.maniculatus. Advisors/Committee Members: David Kass, PhD, Chair, Michael Angell, PhD, Aaron Liepman, PhD.

Subjects/Keywords: Peromyscus maniculatus; retrotransposons; LINE elements; Mys elements; ID element; Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sepehri, S. J. (2011). A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus. (Masters Thesis). Eastern Michigan University. Retrieved from http://commons.emich.edu/theses/310

Chicago Manual of Style (16th Edition):

Sepehri, Saedeh J. “A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus.” 2011. Masters Thesis, Eastern Michigan University. Accessed March 22, 2019. http://commons.emich.edu/theses/310.

MLA Handbook (7th Edition):

Sepehri, Saedeh J. “A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus.” 2011. Web. 22 Mar 2019.

Vancouver:

Sepehri SJ. A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus. [Internet] [Masters thesis]. Eastern Michigan University; 2011. [cited 2019 Mar 22]. Available from: http://commons.emich.edu/theses/310.

Council of Science Editors:

Sepehri SJ. A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus. [Masters Thesis]. Eastern Michigan University; 2011. Available from: http://commons.emich.edu/theses/310


Eastern Michigan University

2. Katakowski, Joseph Adam. The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay.

Degree: MS, Biology, 2007, Eastern Michigan University

Short interspersed DNA elements (SINEs) and long interspersed DNA elements (LINEs) represent two families of transposable elements (TEs) within mammalian genomes. About 45% of the human genome consists of TE derived sequences. SINEs do not encode protein, so they cannot be autonomously propagated and require additional cellular machinery, most likely LINE proteins (ORF1p and ORF2p), for their mobilization, which has been supported by a cell culture-based retrotransposition assay. SINEs are ancestrally derived from either the 7SL RNA gene or from various tRNA genes. Sequence data support the co-evolution of some tRNA-derived SINEs with LINEs. This study involved testing whether or not tRNAderived SINEs could mobilize in the absence of LINE ORF1p, as well as assessing a coevolution of both SINEs and LINEs using the retrotransposition assay. A series of SINE constructs containing Alu, B2, and B1 elements were created and analyzed. No evidence to support a co-evolution of SINEs and LINEs was found; however, it was discovered that tRNA-derived SINEs do not require ORF1p to mobilize. Interestingly, both tRNA and 7SL RNA-derived SINEs mobilize more effectively in the absence of ORF1p. Advisors/Committee Members: David Kass, PhD, Chair, Michael Angell, PhD, Tamara Greco, PhD.

Subjects/Keywords: Mobile genetic elements; Molecular genetics; RNA; DNA; Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Katakowski, J. A. (2007). The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay. (Masters Thesis). Eastern Michigan University. Retrieved from http://commons.emich.edu/theses/14

Chicago Manual of Style (16th Edition):

Katakowski, Joseph Adam. “The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay.” 2007. Masters Thesis, Eastern Michigan University. Accessed March 22, 2019. http://commons.emich.edu/theses/14.

MLA Handbook (7th Edition):

Katakowski, Joseph Adam. “The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay.” 2007. Web. 22 Mar 2019.

Vancouver:

Katakowski JA. The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay. [Internet] [Masters thesis]. Eastern Michigan University; 2007. [cited 2019 Mar 22]. Available from: http://commons.emich.edu/theses/14.

Council of Science Editors:

Katakowski JA. The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay. [Masters Thesis]. Eastern Michigan University; 2007. Available from: http://commons.emich.edu/theses/14

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