Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"Eastern Michigan University" +contributor:("Joseph Paulauskis"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Eastern Michigan University

1. Aldilaimi, Akram. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.

Degree: MS, Health Sciences, 2016, Eastern Michigan University

Next-Generation Sequencing (NGS) technology has advanced the field of personalized medicine by predicting effective treatments for cancer patients using genomic sequence data, including the detection of oncogenes (genes involved in cancer development). Simultaneous amplification of multiple DNA templates (referred to as polyclonal formation) is a primary disadvantage when preparing templates for NGS resulting in redundancies in DNA sequences or nonspecific noise in the sequencing. This study was conducted to compare DNA templates for sequencing prepared using two instruments the Thermo Fisher Ion Chef (IC) and Thermo Fisher OneTouch-2 (OT2). Six sequencing metrics obtained from 114 sequencing trials for evaluation: polyclonal reads, total sequencing reads, empty microcell well reads, no-template reads, useable number reads, and library number reads. A comparison of mean sequencing metrics between the IC and OT2 methods established that for four of the six metrics, IC was the preferred operation for DNA template preparation due to the less polyclonal formation. Advisors/Committee Members: Irwin Martin, David Kass, Joseph Paulauskis.

Subjects/Keywords: DNA Sequence; Genomic sequencing; Next Generation Sequencing; PCR duplications; Personalized Medicine; Polyclonal formation; Medicine and Health Sciences

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Aldilaimi, A. (2016). A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. (Masters Thesis). Eastern Michigan University. Retrieved from https://commons.emich.edu/theses/790

Chicago Manual of Style (16th Edition):

Aldilaimi, Akram. “A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.” 2016. Masters Thesis, Eastern Michigan University. Accessed September 28, 2020. https://commons.emich.edu/theses/790.

MLA Handbook (7th Edition):

Aldilaimi, Akram. “A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues.” 2016. Web. 28 Sep 2020.

Vancouver:

Aldilaimi A. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. [Internet] [Masters thesis]. Eastern Michigan University; 2016. [cited 2020 Sep 28]. Available from: https://commons.emich.edu/theses/790.

Council of Science Editors:

Aldilaimi A. A comparison of two methods of template amplification for next generation sequencing: Implications of polyclonal formation on DNA sequence for several cancer tissues. [Masters Thesis]. Eastern Michigan University; 2016. Available from: https://commons.emich.edu/theses/790

.