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1. Ferooz, Jonathan. Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire.

Degree: 2009, DIAL (Belgium)

The bacterial flagellum is a rotative filamentous appendage which is anchored in the bacterial membranes and allows the bacterium to move. Besides its main locomotive function, the bacterial flagellum fulfills other functions such as adhesion and secretion. The filament, which comprises most of the flagellum structure, is formed by an assembly of proteins called flagellins. It is estimated that nearly 80% of the bacteria produce a flagellum. Brucella is a mammal pathogens bacterium that had long been designated as non-flagellated. However, recent discoveries have shown that all flagellar genes were present in its genome and were necessary to Brucella’s pathogenic ability. Several regulators that control the production of flagellar proteins have also been uncovered. However, no flagellum was viewed on Brucella until today. In this work, we showed for the first time through the optical and electron microscopy that B. melitensis produces transitionally a polar sheathed flagellum. On the other hand, we also investigated the regulation of flagellar system. Firstly, we studied the role of sigma factor RpoE1 on flagellar genes. The rpoE1 mutant overexpresses flagellar genes and produces a longer flagellum. Secondly, we showed that the production of flagellin was controlled by specific regulators named FlbT and FlaF. FlbT is a flagellin activator while FlaF is a flagellin inhibitor. In addition, we showed that the function of FlbT appears to be conserved among the Rizhobiales. We also showed that B. melitensis is more virulent in the absence of flagellin, which indicates a specific role of flagellin. The function of the flagellin of Brucella remains to be investigated. Finally, a preliminary in silico analysis of the genome of Brucella has enabled us to target other potential regulators of the flagellar system. The data gathered during this work have allowed us to draw a hierarchical regulatory cascade of flagellar genes of Brucella in 3 classes.

Le flagelle bactérien est un appendice rotatoire filamenteux ancré dans les membranes bactériennes et permet à la bactérie de se mouvoir. Bien que le rôle locomoteur soit principal, d’autres rôles lui sont attribués comme l’adhérence et la sécrétion. Le filament compose la plus grande partie du flagelle. Celui-ci est formé par un assemblage de protéines appellées flagellines. On estime que près de 80% des bactéries produisent un flagelle. Brucella est une bactérie pathogène de mammifères qui a longtemps été désignée comme non-flagellée. Cependant, les récentes découvertes ont montré que tous les gènes flagellaires étaient présents dans son génome et qu’ils étaient nécessaires lors de l’infection. Plusieurs régulateurs qui contrôlent la production des protéines flagellaires ont également été mis au jour. Néanmoins, aucun flagelle n’avait encore été visualisé chez Brucella. Lors de ce travail, nous avons montré pour la première fois grâce à la microscopie optique et électronique que B. melitensis produit de manière transitoire un flagelle polaire de type gainé. D’autre part, nous…

Advisors/Committee Members: FUNDP - SBIO_URBM (unité de recherche en biologie moléculaire), Letesson, Jean-Jacques, Matroule, Jean-Yves, Michiels, Carine, Cornelis, Pierre, Zygmunt, Michel.

Subjects/Keywords: Brucella; brucellose; flagelle; flagelline; flbT; système flagellaire; mobilité; microbiologie

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APA (6th Edition):

Ferooz, J. (2009). Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire. (Thesis). DIAL (Belgium). Retrieved from http://hdl.handle.net/2078.2/23882

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ferooz, Jonathan. “Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire.” 2009. Thesis, DIAL (Belgium). Accessed February 16, 2019. http://hdl.handle.net/2078.2/23882.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ferooz, Jonathan. “Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire.” 2009. Web. 16 Feb 2019.

Vancouver:

Ferooz J. Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire. [Internet] [Thesis]. DIAL (Belgium); 2009. [cited 2019 Feb 16]. Available from: http://hdl.handle.net/2078.2/23882.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferooz J. Le flagelle de Brucella melitensis : caractérisation de la structure et de la régulation flagellaire. [Thesis]. DIAL (Belgium); 2009. Available from: http://hdl.handle.net/2078.2/23882

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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