+publisher:"Cornell University" +contributor:("Fetcho, Joseph R.")

Cornell University

1. Haft Javaherian, Mohammad. QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS.

Degree: PhD, Biomedical Engineering, 2019, Cornell University

URL: http://hdl.handle.net/1813/67412

► Vasculature networks are responsible for providing reliable blood perfusion to tissues in health or disease conditions. Volumetric imaging approaches, such as multiphoton microscopy, can generate… (more)

Subjects/Keywords: Crowdsourcing; Electrical engineering; computer vision; Image Processing; Biomedical engineering; Artificial intelligence; Alzheimer’s disease; Brain Vasculature; Network Analysis

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APA (6^th Edition):

Haft Javaherian, M. (2019). QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/67412

Chicago Manual of Style (16^th Edition):

Haft Javaherian, Mohammad. “QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS.” 2019. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/67412.

MLA Handbook (7^th Edition):

Haft Javaherian, Mohammad. “QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS.” 2019. Web. 18 Apr 2021.

Vancouver:

Haft Javaherian M. QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS. [Internet] [Doctoral dissertation]. Cornell University; 2019. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/67412.

Council of Science Editors:

Haft Javaherian M. QUANTITATIVE ASSESSMENT OF CEREBRAL MICROVASCULATURE USING MACHINE LEARNING AND NETWORK ANALYSIS. [Doctoral Dissertation]. Cornell University; 2019. Available from: http://hdl.handle.net/1813/67412

Cornell University

2. Rosidi, Nathanael. Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods.

Degree: PhD, Biomedical Engineering, 2011, Cornell University

URL: http://hdl.handle.net/1813/30702

► Microvascular lesions are a common feature in the aging brain and clinical evidence has correlated microvascular pathology with the development of neurodegenerative diseases such as… (more)

▼ Microvascular lesions are a common feature in the aging brain and clinical evidence has correlated microvascular pathology with the development of neurodegenerative diseases such as Alzheimer's disease and dementia. Traditional animal models that replicate hemorrhagic and ischemic lesions in the brain typically affect large regions in the cortex and do not reproduce the small-scale lesions linked to neurodegeneration that likely stem from injuries to single microvessels. Due in part to this lack of smallscale injury animal models, there remains an incomplete understanding of the cellular and pathophysiological dynamics following small-scale vascular lesions, making progress on therapeutic strategies difficult. We used tightly focused femtosecond laser pulses to injure single penetrating arterioles (PA) (i.e., arterioles that plunge into the brain) in the cortex of live anesthetized rodents and used two-photon excited fluorescence (2PEF) imaging to quantify blood flow changes and to determine the time course of pathological consequences in the brain after injury. We find that after ischemic occlusion of a PA, nearby pial and penetrating arterioles do not actively compensate for the reduction of blood flow observed near the occluded blood vessel. We find that capillaries connected downstream to the clotted vessel dilate but other capillaries in the vicinity do not, suggesting that any compensatory signal that results in a physiological response travels vascularly. We ruptured individual PAs to induce microhemorrhages that resulted in extravasation of blood into the parenchyma. We find that tissue compression due to the hematoma does not collapse capillaries and cause acute ischemia. 2PEF imaging of mice expressing yellow fluorescent protein (YFP) in a subset of cortical neurons revealed no dendrite degeneration out to seven days after microhemorrhage. However, we did observe an inflammatory response by microglia/macrophages as quickly as 1.5-hrs after microhemorrhage which persisted past seven days. Lastly, we looked at spine (i.e., post-synaptic terminals on dendrites) dynamics on GFP fluorescent cortical dendrites and found a higher rate of spine loss and gain after a nearby microhemorrhage out to 14 days. This higher rate of spine turnover may help provide an understanding of the development of symptomatic dysfunction due to consequences in neuronal rewiring after a microhemorrhage. The work presented in this dissertation provides quantification of pathological consequences after both ischemic and hemorrhagic injury to a single blood vessel in the brain. We see that after a small-scale ischemic lesion, surrounding blood vessels do not elicit an active response to compensate for a lack of blood flow in the targeted blood vessel and surrounding tissue. After a hemorrhage to a single blood vessel, we do not observe any neuronal degeneration or death. These hemorrhagic lesions, however, do result in an inflammatory reaction that may lead to subtle changes in neuronal rewiring or seed the development of… Advisors/Committee Members: Schaffer, Chris (chair), Xu, Chunhui (committee member), Fetcho, Joseph R. (committee member).

Subjects/Keywords: two photon imaging; hemorrhage; microvascular injury

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APA (6^th Edition):

Rosidi, N. (2011). Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30702

Chicago Manual of Style (16^th Edition):

Rosidi, Nathanael. “Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods.” 2011. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/30702.

MLA Handbook (7^th Edition):

Rosidi, Nathanael. “Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods.” 2011. Web. 18 Apr 2021.

Vancouver:

Rosidi N. Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/30702.

Council of Science Editors:

Rosidi N. Studies Of Pathological Dynamics After Microvascular Injury Using Nonlinear Optical Methods. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30702

Cornell University

3. Nguyen, John. Femtosecond Laser Pulses To Model And Treat Brain Diseases.

Degree: PhD, Biomedical Engineering, 2011, Cornell University

URL: http://hdl.handle.net/1813/30763

► Tightly focusing femtosecond laser pulses into bulk media, such as brain tissue, can generate high intensities within the focal volume. With sufficient laser energies, nonlinear… (more)

▼ Tightly focusing femtosecond laser pulses into bulk media, such as brain tissue, can generate high intensities within the focal volume. With sufficient laser energies, nonlinear absorption of femtosecond laser pulses by the media can take place. This thesis takes advantages of these nonlinear processes to image the cortical vasculature in live anesthetized rodent, and induce localized tissue disruption. We used two-photon excited fluorescence (2PEF) microscopy to image fluorescently labeled cortical vasculature in vivo. In this technique, two-photons are simultaneously absorbed to generate fluorescence that is localized within the focal volume, where intensity is greatest. Scanning the focus enables three-dimensional imaging since out-of-focus fluorescence is not generated. Higher-order nonlinear absorption processes that lead to plasma and cavitation bubble formation are used to induce localized tissue disruption. Together these nonlinear tools enable fine-detailed studies of the cortical vasculature and precise ablation of tissue. To understand the potential role of small venule strokes in the development of cognitive disorders, we investigated the blood flow and vascular effects in individual vessels after inducing single venule occlusions using femtosecond laser ablation. To better understand the blood flow and metabolic responses during an epileptic seizure, we use a several optical techniques to investigate the neurovascular and neurometabolic activity during pre-ictal and ictal phases of focal seizures. Using 2PEF microscopy we reveal the presence of vasoconstriction in arterioles surrounding the focus prior to seizure onset. The deposition of energy within the focal volume can also used to make incisions in bulk brain to disrupt tissue. Using our laser scalpel, we produced sub-surface cortical incisions and characterized the cut width and depth as a function of different laser energies. We then employed this cutting technique to determine the efficacy of sub-surface cuts in stopping seizure propagation. We then showcased a technique to produce welds at the unexposed interface between two pieces of glass and characterized their size as a function of multiple experimental parameters. Joint strength of the fused glass was tested in addition to investigating the changes in optical properties induced by the weld. Lastly, future experiments and advantages of nonlinear optical techniques are discussed. Advisors/Committee Members: Schaffer, Chris (chair), Fetcho, Joseph R. (committee member), Zipfel, Warren R. (committee member).

Subjects/Keywords: two-photon microscopy; femtosecond laser ablation; hemodynamics

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APA (6^th Edition):

Nguyen, J. (2011). Femtosecond Laser Pulses To Model And Treat Brain Diseases. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30763

Chicago Manual of Style (16^th Edition):

Nguyen, John. “Femtosecond Laser Pulses To Model And Treat Brain Diseases.” 2011. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/30763.

MLA Handbook (7^th Edition):

Nguyen, John. “Femtosecond Laser Pulses To Model And Treat Brain Diseases.” 2011. Web. 18 Apr 2021.

Vancouver:

Nguyen J. Femtosecond Laser Pulses To Model And Treat Brain Diseases. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/30763.

Council of Science Editors:

Nguyen J. Femtosecond Laser Pulses To Model And Treat Brain Diseases. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30763

Cornell University

4. Kishore, Sandeep. The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish.

Degree: PhD, Neurobiology, 2011, Cornell University

URL: http://hdl.handle.net/1813/33616

► The work described here focuses on novel patterns of motoneuron dendritic organization and dendritic development in relation to the recruitment patterns of spinal motoneurons that… (more)

▼ The work described here focuses on novel patterns of motoneuron dendritic organization and dendritic development in relation to the recruitment patterns of spinal motoneurons that drive swimming in larval zebrafish. I first looked at the dendritic organization of motoneurons in freely swimming fish and tracked its emergence in relation to the maturation of locomotor behavior. I used transient expression of fluorescent proteins to visualize the dendritic structure of motoneurons in zebrafish larvae at a stage when they have been swimming freely for a few days. My work showed that there is a dendritic topography related to the recruitment of motoneurons at different locomotor speeds that emerges by the time fish first begin to swim, and is maintained even as dendrites grow after the onset of spontaneous swimming. Since neuronal activity is thought to influence dendritic structure, I then studied the structural dynamics of dendritic arbors of individual motoneurons in larval zebrafish soon after they begin swimming. I found a systematic relationship between the location of a spinal motoneuron and the dynamics of its dendritic arbor - youngest, ventral motoneurons are least dynamic whereas increasingly older and more dorsal motoneurons are more dynamic. This is contrary to the idea that dendrites of younger neurons are more dynamic than dendrites of older neurons because younger ones are growing more. I then asked if this pattern of dendritic dynamics is related to the systematic variation of excitability of motoneurons described recently. I tested this possibility genetically by expressing Kir2.1 to suppress excitability of individual motoneurons. This led to a dramatic increase in the dynamics of ventral motoneurons, which became more dynamic than more dorsal ones. My results suggest that a naturally occurring dorsoventral gradient of excitability may contribute to the variation in dendritic dynamics. The patterns of dendritic organization and development I describe may also be applicable to other interneuron types in the spinal cord and hindbrain. Advisors/Committee Members: Fetcho, Joseph R. (chair), Deitcher, David Lawrence (committee member), Harris-Warrick, Ronald Morgan (committee member).

Subjects/Keywords: Dendrites; Motoneurons; Zebrafish; Development; Filopodia; Imaging; Motor networks

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APA (6^th Edition):

Kishore, S. (2011). The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/33616

Chicago Manual of Style (16^th Edition):

Kishore, Sandeep. “The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish.” 2011. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/33616.

MLA Handbook (7^th Edition):

Kishore, Sandeep. “The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish.” 2011. Web. 18 Apr 2021.

Vancouver:

Kishore S. The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/33616.

Council of Science Editors:

Kishore S. The Development And Organization Of Dendrites Of Motoneurons In Larval Zebrafish. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/33616

Cornell University

5. Bares, Amanda Josephine. Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures.

Degree: PhD, Biomedical Engineering, 2017, Cornell University

URL: http://hdl.handle.net/1813/56781

► Studies of both normal and disease state physiology require the visualization of multiple cells and cell types simultaneously. Multiphoton microscopy has enabled researchers to visualize… (more)

▼ Studies of both normal and disease state physiology require the visualization of multiple cells and cell types simultaneously. Multiphoton microscopy has enabled researchers to visualize fluorescently-tagged cells with subcellular resolution in live animal models of disease, overcoming the optical-scattering effects of tissue. However, most multiphoton microscopes only provide two or four color channels, limiting the number of fluorescent labels, and thus cell types, that can be simultaneously imaged. This thesis describes the development and demonstration of a hyperspectral multiphoton microscope that enables simultaneous visualization of multiple cell types. Three laser excitation sources are multiplexed with multiple detection channels, each providing improved spectral detection through the use of angle-tuned bandpass filters. The detection system was designed to provide efficient detection of highly-scattered light from tissue while minimizing the impact of scattered light on spectral resolution. We demonstrated the ability of the instrument to image multiple, spectrally-similar fluorescent labels, and developed methodology to post-process data to generate images with each fluorescent label clearly separated and indicated with a unique color in a composite image. We demonstrated hyperspectral imaging and spectral separation of ten overlapping colors of fluorescent beads, up to seven fluorescent labels in live cells, and five labels in live mouse cortex. In addition, we demonstrated multicolor labeling techniques enabling identification of morphologically-similar cells based on color “hue”, and characterized color variability using the spectral capabilities of the microscope. In other work in this thesis, we explored third harmonic generation for label-free visualization of myelinated nerves over large viewing areas for eventual surgery room applications. Nerves are notoriously difficult to see in the surgical field, and nerve injuries are a common cause of post-surgery morbidity. Third harmonic generation has been established as an excellent tool for myelin visualization, but the requirements for a high zoom microscope objective have limited the area of imaging to areas too small for surgery room use. We demonstrated third harmonic generation imaging in both mouse sciatic nerve and rat cavernous nerve on the prostate with low-zoom, low numerical-aperture objectives, and found that myelin produces less signal than fat deposits, potentially limiting the utility for nerve visualization in areas with high fat content. Advisors/Committee Members: Schaffer, Chris (chair), Fetcho, Joseph R. (committee member), Xu, Chunhui (committee member).

Subjects/Keywords: Biophysics; Biomedical engineering; Nonlinear Optics; Neurosciences; Hyperspectral; Imaging; Microscopy; Multiphoton; Multiplexing

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APA (6^th Edition):

Bares, A. J. (2017). Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/56781

Chicago Manual of Style (16^th Edition):

Bares, Amanda Josephine. “Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures.” 2017. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/56781.

MLA Handbook (7^th Edition):

Bares, Amanda Josephine. “Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures.” 2017. Web. 18 Apr 2021.

Vancouver:

Bares AJ. Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures. [Internet] [Doctoral dissertation]. Cornell University; 2017. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/56781.

Council of Science Editors:

Bares AJ. Development of Tools for Nonlinear Optical Imaging of Multiple Cells and Tissue Structures. [Doctoral Dissertation]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/56781

Cornell University

6. Gadamsetty, Poornima. SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY.

Degree: PhD, Biomedical Engineering, 2017, Cornell University

URL: http://hdl.handle.net/1813/51628

► Optoporation allows the minimal invasive transfer of extracellular molecules into cells. The use of a low repetition rate laser system (100 fs at 790 nm… (more)

▼ Optoporation allows the minimal invasive transfer of extracellular molecules into cells. The use of a low repetition rate laser system (100 fs at 790 nm and 1 kHz repetition rate) can enable the future transfer of this technique to in vivo applications. However, it is essential to understand the biophysical parameter space of this technique beforehand. We examined the optoporation dependence on the extracellular calcium concentration in HeLa cells. We observed a low cell recovery rate in calcium free medium and varying cell viabilities with different calcium concentrations, possibly related to calcium dependent repair processes. Also we followed the calcium rise in the cell by a fluorescent calcium indicator. We extended our previous model of pore formation model and applied different sized dextrans to verify our assumptions on pore radius, and resealing time. Therefore, this study provides a better understanding of the biophysical processes accompanying single cell laser transfection. Drug delivery into the brain is inhibited by the blood brain barrier and limited by the structure of the brain interstitium. Convection-enhanced delivery (CED) is a technique that delivers therapeutics by infusing directly into brain parenchyma through a needle inserted into the brain. We developed a fast scanning, real time volumetric imaging platform to image transport of nanoparticles in the rat cortex in vivo with high temporal and spatial resolution and to study transport mechanisms for different sized nanoparticles in the brain interstitium and the perivascular space (PVS). Fluorescent polystyrene beads of sizes ranging from 20nm to 200nm were infused directly into a rat cortex. Particles sized >100nm had distribution routes along the perivascular spaces and were relatively hindered in the extracellular spaces in comparison to particles in the 20nm – 40nm size range that prefered transport in extracellular space. Thus, we can study the alterations in the shape, size and movement of the infusion in the brain, allowing for better control for potential clinical design and optimization. Advisors/Committee Members: Schaffer, Chris B (chair), Fetcho, Joseph R (committee member), Xu, Chunhui (committee member).

Subjects/Keywords: Biophysics; Biomedical engineering; blood brain barrier; convection enhanced delivery; optoporation; single cell manipulation; two-photon microscopy; Neurosciences

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APA (6^th Edition):

Gadamsetty, P. (2017). SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/51628

Chicago Manual of Style (16^th Edition):

Gadamsetty, Poornima. “SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY.” 2017. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/51628.

MLA Handbook (7^th Edition):

Gadamsetty, Poornima. “SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY.” 2017. Web. 18 Apr 2021.

Vancouver:

Gadamsetty P. SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY. [Internet] [Doctoral dissertation]. Cornell University; 2017. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/51628.

Council of Science Editors:

Gadamsetty P. SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY. [Doctoral Dissertation]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/51628

Cornell University

7. Farrar, Matthew. Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System.

Degree: PhD, Physics, 2012, Cornell University

URL: http://hdl.handle.net/1813/31104

► The spinal cord of vertebrates serves as the conduit for somatosensory information and motor control, as well as being the locus of neural circuits that… (more)

▼ The spinal cord of vertebrates serves as the conduit for somatosensory information and motor control, as well as being the locus of neural circuits that govern fast reflexes and patterned behaviors, such as walking in mammals or swimming in fish. Consequently, pathologies of the spinal cord -such as spinal cord injury (SCI)- lead to loss of motor control and sensory perception, with accompanying decline in life expectancy and quality of life. Despite the devastating effects of these diseases, few therapies exist to substantially ameliorate patient outcome. In part, studies of spinal cord pathology have been limited by the inability to perform in vivo imaging at the level of cellular processes. The focus of this thesis is to present the underlying theory for and demonstration of novel multi-photon microscopy (MPM) and optical manipulation techniques as they apply to studies the mouse central nervous system (CNS), with an emphasis on the spinal cord. The scientific findings which have resulted from the implementation of these techniques are also presented. In particular, we have demonstrated that third harmonic generation is a dyefree method of imaging CNS myelin, a fundamental constituent of the spinal cord that is difficult to label using exogenous dyes and/or transgenic constructs. Since gaining optical access to the spinal cord is a prerequisite for spinal cord imaging, we review our development of a novel spinal cord imaging chamber and surgical procedure which allowed us to image for multiple weeks following implantation without the need for repeated surgeries. We also have used MPM to characterize spinal venous blood flow before and after point occlusions. We review a novel nonlinear microscopy technique that may serve to show optical interfaces in three dimensions inside scattering tissue. Finally, we discuss a model and show results of optoporation, a means of transfecting cells with genetic constructs. Brief reviews of MPM and SCI are also presented. Advisors/Committee Members: Cohen, Itai (chair), Wang, Michelle D (committee member), Elser, Veit (committee member), Schaffer, Chris (committee member), Fetcho, Joseph R. (committee member).

Subjects/Keywords: spinal cord injury; multi-photon microscopy; nonlinear optics

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APA (6^th Edition):

Farrar, M. (2012). Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/31104

Chicago Manual of Style (16^th Edition):

Farrar, Matthew. “Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System.” 2012. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/31104.

MLA Handbook (7^th Edition):

Farrar, Matthew. “Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System.” 2012. Web. 18 Apr 2021.

Vancouver:

Farrar M. Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System. [Internet] [Doctoral dissertation]. Cornell University; 2012. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/31104.

Council of Science Editors:

Farrar M. Nonlinear Optical Techniques For Imaging And Manipulating The Mouse Central Nervous System. [Doctoral Dissertation]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31104

Cornell University

8. Peace, Shane. Novel Insights Into Olfactory Bulb Gamma Oscillations.

Degree: PhD, Neurobiology, 2015, Cornell University

URL: http://hdl.handle.net/1813/41028

► In the rodent olfactory bulb (OB), coherent gamma oscillations (40-100 Hz) in the local field potential are readily observed during active odor investigation and are… (more)

▼ In the rodent olfactory bulb (OB), coherent gamma oscillations (40-100 Hz) in the local field potential are readily observed during active odor investigation and are thought to be necessary for high resolution odorant constructions. These oscillations are believed to be coordinated by dendrodendritic connections in the external plexiform layer (EPL) where the excitatory mitral cells receive inhibitory, GABAergic feedback from granule cells. Currently, the predominant theories of gamma oscillation rhythmogenesis are insufficient to fully describe the complex characteristics of bulbar gamma oscillations. The goal of the presented research was to utilize novel recording techniques to more fully describe the network mechanisms that generate gamma oscillations in the rodent OB. To investigate synchronous activity in the OB, we recorded from OB slices from mice coexpressing channelrhodopsin-2 and enhanced yellow fluorescent protein under the control of the olfactory marker protein, hence limiting expression to the olfactory sensory neuron axonal arbors innervating the glomerular layer of the slices. To enable large area recordings across the olfactory bulb slice, we used a 60-electrode planar microelectrode array to record spike and local field activity. A brief (5 second) flash (4 Hz) of a blue light stimulus or the brief application of metabotropic glutamate receptor agonists and an acetylcholine receptor agonist were used to induce long-lasting (>5 minutes) gamma oscillations (20-55Hz) that were coherent across ranges of up to 300 [MICRO SIGN]m across the OB slice. Contrary to current theories of OB gamma oscillogenesis, blocking bulbar GABA(A) receptor activity did not reduce the power of the gamma band oscillations but did reduce the oscillation frequency. Furthermore, GABAergic blockade eliminated the coherence between oscillating glomerular columns. To explain these results, I propose a hybrid coupled-oscillator model of bulbar oscillogenesis, in which lateral GABAergic feedback couples and synchronizes independent intraglomerular oscillators that rely on non-GABAergic coordination mechanisms, primarily mitral cell subthreshold oscillations (STOs). These STOs function to synchronize the spiking of the mitral cells. Our model predicts the gamma oscillation frequency is influenced by the repetitive GABAergic reset of mitral cell STOs increasing the overall frequency of the oscillatory network. Extensive GABAergic feedback across the external plexiform layer is further necessary to synchronize the intraglomerular oscillations across the bulb producing the coherent gamma oscillations recorded in vivo. To increase the resolution of electrophysiological recordings within the bulbar region producing coherent oscillations, we developed a 768-channel MEA designed with decreased distance between recording electrodes. Our MEA also employed angle sensitive pixels that are optical receptors capable of detecting and reconstructing the patterns of optical stimulation used to excite the transgenic slices. These sensors were… Advisors/Committee Members: Cleland,Thomas A. (chair), Linster,Christiane (committee member), Molnar,Alyosha Christopher (committee member), Fetcho,Joseph R. (committee member).

Subjects/Keywords: olfactory bulb; gamma oscillations; MEA

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APA (6^th Edition):

Peace, S. (2015). Novel Insights Into Olfactory Bulb Gamma Oscillations. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/41028

Chicago Manual of Style (16^th Edition):

Peace, Shane. “Novel Insights Into Olfactory Bulb Gamma Oscillations.” 2015. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/41028.

MLA Handbook (7^th Edition):

Peace, Shane. “Novel Insights Into Olfactory Bulb Gamma Oscillations.” 2015. Web. 18 Apr 2021.

Vancouver:

Peace S. Novel Insights Into Olfactory Bulb Gamma Oscillations. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/41028.

Council of Science Editors:

Peace S. Novel Insights Into Olfactory Bulb Gamma Oscillations. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/41028

Cornell University

9. Byrnes, Laura. Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin.

Degree: PhD, Biophysics, 2013, Cornell University

URL: http://hdl.handle.net/1813/34301

► The membrane of the endoplasmic reticulum (ER) is the site of numerous complex activities essential to the survival of eukaryotic cells. In the membrane sheets… (more)

▼ The membrane of the endoplasmic reticulum (ER) is the site of numerous complex activities essential to the survival of eukaryotic cells. In the membrane sheets of the rough ER (rER), integral membrane proteins are synthesized, folded, modified, and transported throughout the cell. In the smooth ER (sER) tubules, lipids are synthesized and processed with a variety of functionalities, and trafficked to specific membrane compartments. The sER is also the site of calcium storage and controlled release, an important function for several cell types including neurons and muscle. The sER requires energy and the action of numerous proteins to maintain its highly curved, tubular shape and its reticular, interconnected structure. The recent discovery of a family of proteins called atlastins has helped answer some of the questions surrounding the tubular, reticular nature of the sER. Atlastins comprise a group of ER resident proteins that have been shown to facilitate the fusion of membrane tubules within this organelle. They are part of the dynamin superfamily of proteins, which use the energy stored in GTP to sculpt membranes throughout the cell. However, the exact molecular mechanism for how atlastin mediates membrane fusion remained mysterious. In this study, we have taken apart and analyzed atlastin-1, one of three isoforms of atlastin in humans. This isoform is found primarily in the central nervous system and is mutated in the neurodegenerative disorders Hereditary Spastic Paraplegia and Hereditary Sensory Neuropathy. This study has resulted in a collection of three-dimensional, high-resolution structures of atlastin-1's catalytic core fragment, comprising its GTPase and middle domains bound to various forms of the guanine nucleotide. These structures revealed key information about the catalytic mechanism of atlastin-1, as well as interesting and important conformational changes that occur within the structure. Using these structures, as well as information from small-angle X-ray scattering (SAXS), sizeexclusion chromatography coupled to multi-angle light scattering (SEC-MALS), Förster resonance energy transfer (FRET), and enzymatic activity assays, we have put together a general working model for atlastin membrane fusion and have gathered important information about its mechanism that may be used to target the protein for the treatment of disease. Advisors/Committee Members: Sondermann, Holger (chair), Fetcho, Joseph R. (committee member), Brown, William J (committee member), Feigenson, Gerald W (committee member).

Subjects/Keywords: atlastin; dynamin; membrane fusion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Byrnes, L. (2013). Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/34301

Chicago Manual of Style (16^th Edition):

Byrnes, Laura. “Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin.” 2013. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/34301.

MLA Handbook (7^th Edition):

Byrnes, Laura. “Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin.” 2013. Web. 18 Apr 2021.

Vancouver:

Byrnes L. Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/34301.

Council of Science Editors:

Byrnes L. Molecular Basis For Nucleotide-Dependent Conformational Changes Of The Large Gtpase Atlastin. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34301

Cornell University

10. Staiger, Elizabeth. Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior.

Degree: PhD, Animal Science, 2015, Cornell University

URL: http://hdl.handle.net/1813/39327

► Selection after domestication has primarily focused on performance, conformation and desirable behaviors in the horse, resulting in breeds that are divergent across these traits. An… (more)

▼ Selection after domestication has primarily focused on performance, conformation and desirable behaviors in the horse, resulting in breeds that are divergent across these traits. An example are the "gaited" breeds, horses with the ability to perform either a lateral or diagonal fourbeat gait without a moment of suspension at intermediate speeds, yet varying in overall size and temperament. To investigate the contribution of genetics to these divergent traits, we collected DNA samples, 35 body measurements, gait information, horse discipline, and a behavior survey from 801 gaited horses. Utilizing previously genotyped horses, an across-breed genome-wide association study (GWA) identified three novel candidate regions associated with gait type on ECA1, ECA11, and EC4. A GWA in a single gaited breed, the Tennessee Walking Horse (TWH) identified two additional candidate regions on ECA19 and ECA11. Polymorphisms from whole-genome sequences have identified several SNPs within these candidate regions. We conducted principle component analysis (PCA) on 33 of the body measures for a subset of TWH. A GWA of the first PC, which describes overall size, identified the LCORL locus, which has previously been implicated with size in horses, cattle, and humans. No causal variant has been discovered yet due to extensive linkage disequilibrium (LD) in the region, but LD in the TWH is much lower, improving the resolution capabilities for fine-mapping and variant discovery. To investigate the contribution of genetics to temperament, we utilized factor analysis (FA) on the questionnaire to identify four temperament factors in TWH: neophobia, trainability, hostility, and independence. These four factors account for 64% of the total trait variance. We ran three separate GWAs using the F1-'neophobia', F2-'trainability', and F3-'hostility' scores as the phenotype and identified candidate markers in genes involved with neurodegeneration, steroidogenesis, brain development, and neuronal cell signaling pathways. The results from this work will hopefully lead to future studies to identify the causal variants of locomotion, size, and behavior traits. This will allow for the development of genetic tests to aid horse owners in their breeding and management decisions and help improve horse welfare as horses are selected for appropriate disciplines. Advisors/Committee Members: Brooks, Samantha A. (chair), Huson, Heather Jay (committee member), Fetcho, Joseph R. (committee member), Cheetham, Jonathan (committee member).

Subjects/Keywords: Genetics; Horses; Gait

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Staiger, E. (2015). Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/39327

Chicago Manual of Style (16^th Edition):

Staiger, Elizabeth. “Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior.” 2015. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/39327.

MLA Handbook (7^th Edition):

Staiger, Elizabeth. “Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior.” 2015. Web. 18 Apr 2021.

Vancouver:

Staiger E. Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/39327.

Council of Science Editors:

Staiger E. Polymorphic Gait In The Horse: An Interaction Of Genetics, Morphology, And Behavior. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/39327

Cornell University

11. Gallant, Jason. Mechansims Of Signal Diversity In Mormyrid Electric Fish.

Degree: PhD, Behavioral Biology, 2011, Cornell University

URL: http://hdl.handle.net/1813/30673

► Mormyrid fishes are one of six independent lineages of vertebrates to have evolved electric organs from skeletal muscle. The physiological output of the electric organ… (more)

▼ Mormyrid fishes are one of six independent lineages of vertebrates to have evolved electric organs from skeletal muscle. The physiological output of the electric organ is an electric signal called the electric organ discharge (EOD). EODs are especially diverse among mormyrids, particularly among a rapidly diverged, geographically restricted species flock Paramormyrops. Recent studies have implicated that EODs may be a contributing factor to the process of species divergence in Paramormyrops. In this dissertation, I have examined the genetic basis of differences between skeletal muscle and electric organ in mormyrids, and the population-level processes that contribute to their diverse physiological outputs of electric organs. In Chapter 1, I provide a general overview of electric organs, with specific attention to the developmental and genetic mechanisms underlying their development. Chapter 1 concludes with a discussion of evolutionary processes responsible for signal diversification. In Chapter 2, I describe the use of suppressive subtractive hybridization to identify genes differentially expressed in the electric organ vs. skeletal muscle in the mormyrid Brienomyrus brachyistius. This work provides a basis for future comparative work with other electric fishes. In Chapter 3, I describe patterns of geographic variation in EOD signaling among a geographically widespread member of the Paramormyrops species flock, P. kingsleyae, and describe morphological correlates of this variation. In Chapter 4, I use a population genetics approach to analyze data concerning signal variation, behavior, and anatomy in Paramormyrops kingsleyae to specifically test the hypothesis that the unique patterns of geographic variation and signal divergence detected in Chapter 3 are the result of geographic barriers and distance that reduce gene flow between local populations. Advisors/Committee Members: Hopkins, Carl D (chair), Deitcher, David Lawrence (coChair), McCune, Amy R. (committee member), Shaw, Kerry L (committee member), Fetcho, Joseph R. (committee member).

Subjects/Keywords: mormyrid; electric fish; Paramormyrops

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gallant, J. (2011). Mechansims Of Signal Diversity In Mormyrid Electric Fish. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30673

Chicago Manual of Style (16^th Edition):

Gallant, Jason. “Mechansims Of Signal Diversity In Mormyrid Electric Fish.” 2011. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/30673.

MLA Handbook (7^th Edition):

Gallant, Jason. “Mechansims Of Signal Diversity In Mormyrid Electric Fish.” 2011. Web. 18 Apr 2021.

Vancouver:

Gallant J. Mechansims Of Signal Diversity In Mormyrid Electric Fish. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/30673.

Council of Science Editors:

Gallant J. Mechansims Of Signal Diversity In Mormyrid Electric Fish. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30673

Cornell University

12. Lawton, Kristy. Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song.

Degree: PhD, Neurobiology, 2014, Cornell University

URL: http://hdl.handle.net/1813/36103

► FoxP2 is a transcription factor underlying a severe human speech and language disorder. Research on this gene in vertebrate model organisms such as mouse and… (more)

▼ FoxP2 is a transcription factor underlying a severe human speech and language disorder. Research on this gene in vertebrate model organisms such as mouse and zebra finch indicated that it is highly conserved between species and strongly expressed in motor related brain structures. Although these studies implicated FoxP2 in development of motor control regions, the precise mechanisms are not understood. This work is the first to explore the functional role of FoxP2's Drosophila homolog known as FoxP. We characterize the behavioral importance of this gene in flies using FoxP specific RNA interference throughout development, which impairs several types of behavior, with males more strongly affected. We also used a temperature sensitive UAS-Shibire line to disrupt FoxP neuron function in adulthood and we saw dramatic effects on motor coordination. Our work also provides the first visualization of FoxP expression in the fly brain. We see a small number of symmetrically expressed FoxP neurons in clusters throughout the brain, but no obvious difference between males and females. There is also strong FoxP expression in the protocerebral bridge within the central complex, which is essential for higher level locomotion control and is thought to be homologous to the vertebrate basal ganglia. FoxP2 in humans is also highly expressed in the basal ganglia, specifically in areas important for motor coordination. These results indicate a conserved functional homology of this gene between invertebrates and vertebrates, both in terms of behavioral effects on motor coordination as well as expression pattern. We also disrupted FoxP neurons throughout pupal stages and discover that eclosion behavior is abolished, indicating a possible role of FoxP in development during pupation. Thus we propose to establish Drosophila as a model to study this crucial speech disease gene. We believe this work will contribute to further understanding of the importance of FoxP transcription factors in humans, as well as provide further support for the idea of deep homology between the invertebrate and vertebrate brain. Advisors/Committee Members: Deitcher, David Lawrence (chair), Fetcho, Joseph R. (committee member), Hoy, Ronald Raymond (committee member), Bass, Andrew Howard (committee member).

Subjects/Keywords: FoxP; Drosophila melanogaster; courtship song

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lawton, K. (2014). Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36103

Chicago Manual of Style (16^th Edition):

Lawton, Kristy. “Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song.” 2014. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/36103.

MLA Handbook (7^th Edition):

Lawton, Kristy. “Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song.” 2014. Web. 18 Apr 2021.

Vancouver:

Lawton K. Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/36103.

Council of Science Editors:

Lawton K. Conserved Role Of Drosophila Melanogaster Foxp In Motor Coordination And Courtship Song. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36103

Cornell University

13. Seo, Changwoo. Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode.

Degree: PhD, Neurobiology and Behavior, 2020, Cornell University

URL: http://hdl.handle.net/1813/102980

► Survival depends on the selection of behaviors adaptive for the current environment. For example, a mouse should run from a rapidly looming hawk but should… (more)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seo, C. (2020). Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/102980

Chicago Manual of Style (16^th Edition):

Seo, Changwoo. “Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode.” 2020. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/102980.

MLA Handbook (7^th Edition):

Seo, Changwoo. “Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode.” 2020. Web. 18 Apr 2021.

Vancouver:

Seo C. Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode. [Internet] [Doctoral dissertation]. Cornell University; 2020. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/102980.

Council of Science Editors:

Seo C. Intense threat switches dorsal raphe serotonin system to a paradoxical operational mode. [Doctoral Dissertation]. Cornell University; 2020. Available from: http://hdl.handle.net/1813/102980

14. DiPietro, Joseph V. The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence.

Degree: PhD, Neurobiology and Behavior, 2017, Cornell University

URL: http://hdl.handle.net/1813/56717

► The work presented in this dissertation is an in-depth analysis of how a single neuron, the primary motoneuron of zebrafish, searches for and forms synapses… (more)

▼ The work presented in this dissertation is an in-depth analysis of how a single neuron, the primary motoneuron of zebrafish, searches for and forms synapses across the circadian periods of activity and quiescence that occur from day to night. Dendrites repeatedly extend and retract filopodia in a search for synapses, the formation of which stabilizes the process, leading to growth of the arbor. I first focused my attention on zebrafish primary neurons during the day to explore, for the first time, the search process from the level of individual filopodial dynamics to the distribution of dynamics across an entire dendritic arbor. We found the magnitude of searching at individual locations varied tremendously across locations, with filopodia extending and retracting as much as 3 microns and averaging about 12 dynamics events per filopodium in a given 30-minute period. An analysis of the temporal sequence of these dynamic events showed the pattern of dynamics at individual locations also varied tremendously. Only retractions showed a consistent trend, tending to stabilize for some time and rarely being followed by an extension. This shows that dendrites are not just simply extending and stabilizing filopodia. Instead, filopodia at each individual location are engaging in highly dynamic periods of searching, with most extensions retracting within 5 minutes. Only about 4% of extensions survive for an hour or more, possibly representing the formation of synapses. We further find that these dynamic locations are equally distributed across the dendrite arbor independent of their individual dynamics and only the furthest extents of dendrite had higher than average numbers of motile locations, suggesting the search is largely unbiased except for those furthest regions. A comparison of how the search differs between day and night shows surprisingly, that the magnitude of this searching increases at night, a quiescent time, when motoneuron are much less active. While the extensions at night are less likely to stabilize, this increase in searching may partly account for these cells still forming synapses at a similar rate as during the day, with only the rate of synapse removal changing, increasing significantly at night. These results demonstrate that during times of behavioral and activity quiescence, neurons continue to search for and form synapses at the expense of losing others, providing potential insight into the role these quiescent states may play in network development and neuronal plasticity. Advisors/Committee Members: Fetcho, Joseph R. (chair), Harris-Warrick, Ronald Morgan (committee member), Bass, Andrew Howard (committee member), Schaffer, Chris (committee member).

Subjects/Keywords: dendrites; motoneurons; sleep; synapses; zebrafish; Development; Neurosciences

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

DiPietro, J. V. (2017). The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/56717

Chicago Manual of Style (16^th Edition):

DiPietro, Joseph V. “The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence.” 2017. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/56717.

MLA Handbook (7^th Edition):

DiPietro, Joseph V. “The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence.” 2017. Web. 18 Apr 2021.

Vancouver:

DiPietro JV. The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence. [Internet] [Doctoral dissertation]. Cornell University; 2017. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/56717.

Council of Science Editors:

DiPietro JV. The Search for and Formation of Synapses on Zebrafish Motoneurons Across Natural Periods of Activity and Quiescence. [Doctoral Dissertation]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/56717

Cornell University

15. Wray, Margaret. Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level.

Degree: PhD, Neurobiology, 2011, Cornell University

URL: http://hdl.handle.net/1813/30693

Subjects/Keywords: Honey bee; Animal personality; Animal cognition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wray, M. (2011). Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30693

Chicago Manual of Style (16^th Edition):

Wray, Margaret. “Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level.” 2011. Doctoral Dissertation, Cornell University. Accessed April 18, 2021. http://hdl.handle.net/1813/30693.

MLA Handbook (7^th Edition):

Wray, Margaret. “Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level.” 2011. Web. 18 Apr 2021.

Vancouver:

Wray M. Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1813/30693.

Council of Science Editors:

Wray M. Personality And Cognition In Honey Bees (Apis Mellifera) At The Individual And Colony Level. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30693

Open Access Theses and Dissertations