+publisher:"Cornell University" +contributor:("Cohen, Paula")

Cornell University

1. Strong, Edward. Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse .

Degree: 2014, Cornell University

► It is clear that there are many genes required for meiosis in mammals that are not present in the more tractable model organisms. To identify… (more)

Subjects/Keywords: Meiosis; Sumoylation; CCNB1IP1

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APA (6^th Edition):

Strong, E. (2014). Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/36075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Strong, Edward. “Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse .” 2014. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/36075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Strong, Edward. “Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse .” 2014. Web. 13 Dec 2019.

Vancouver:

Strong E. Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse . [Internet] [Thesis]. Cornell University; 2014. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/36075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Strong E. Towards The Understanding Of Ccnb1Ip1 As A Co-Regulator Of Meiotic Crossing-Over In The Mouse . [Thesis]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

2. Brosnahan, Margaret. Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/39386

► Successful mammalian pregnancy requires precise modulation of normal biological processes to ensure the survival of the feto-placental tissues while preserving the good health of the… (more)

▼ Successful mammalian pregnancy requires precise modulation of normal biological processes to ensure the survival of the feto-placental tissues while preserving the good health of the mother. The original research presented in this dissertation encompasses studies in both of these areas. The first part of this work addresses the long-standing question as to how feto-placental tissues, specifically invasive trophoblast, survive in the hostile environment of the maternal immune system. The Antczak laboratory previously developed an ectopic trophoblast transplant system to facilitate studies of the maternal immune response to invasive trophoblast in the horse. Single transplants of horse chorionic girdle into the vulvar mucosa of non-pregnant horse recipients were found to have lifespans and physiologic effects upon recipients similar to endometrial cups in a normal horse pregnancy. The current studies first expanded the trophoblast transplant system to include multiple rather than single transplants in individual recipients. The objective of this was to examine ectopic trophoblast survival in an immunologically primed as compared to naïve recipient. These serial transplants had lifespans similar to the single transplants, suggesting that the mechanisms protecting the trophoblast from immune destruction are initiated by the trophoblast itself. A series of transplants then was performed using Jenny donkeys as recipients. In each donkey recipient, primary transplants had lifespans of similar length to the horse-to-horse transplants, with second and subsequent transplants having progressively shorter lifespans. This pattern suggests that the horse-to-donkey transplants were subjected to a classic destructive memory response. The second body of work addresses the question as to how normal placentation disrupts maternal anatomy and physiology without causing adverse effects. This work was initiated with the discovery of interleukin 22 (IL22) mRNA in day 34 chorionic girdle using gene expression array analysis. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) then revealed that IL22 mRNA was rapidly upregulated in the chorionic girdle between days 32 and 35 of pregnancy. This represented the first identification of IL22 expression by a non-immune cell type. Interleukin 22 receptor RA1 (IL22RA1) mRNA was identified in pregnant endometrium, and in situ hybridization localized the mRNA to both the luminal and glandular epithelia. A monoclonal antibody was then generated against horse IL22. Mice were immunized with a fusion protein containing recombinant horse IL4 and IL22. Murine splenic cells were fused with SP 2/O myeloma cells to generate hybridoma cell lines. A monoclonal antibody to equine IL22 was identified. IL22 protein was then successfully detected in day 35 chorionic girdle. Knowing that IL22 in other biological systems has a role in preserving the integrity of mucosal surfaces and in epithelial cell proliferation, it was hypothesized that IL22 produced from the chorionic girdle binds receptor in… Advisors/Committee Members: Cohen, Paula (committeeMember), Wagner, Bettina (committeeMember).

Subjects/Keywords: equine; trophoblast; immunology

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APA (6^th Edition):

Brosnahan, M. (2015). Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/39386

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Brosnahan, Margaret. “Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle .” 2015. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/39386.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Brosnahan, Margaret. “Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle .” 2015. Web. 13 Dec 2019.

Vancouver:

Brosnahan M. Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/39386.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Brosnahan M. Invasion And Survival: Immunological Studies Of The Equine Chorionic Girdle . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/39386

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

3. Mainiero, Samantha. Understanding How Chromosome Structure Influences Recombination .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/40718

► Meiotic recombination results in the exchange of DNA between homologous chromosomes, creating allelic diversity while at the same time ensuring correct segregation of chromosomes during… (more)

Subjects/Keywords: meiosis; chromosome; maize

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mainiero, S. (2015). Understanding How Chromosome Structure Influences Recombination . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mainiero, Samantha. “Understanding How Chromosome Structure Influences Recombination .” 2015. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/40718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mainiero, Samantha. “Understanding How Chromosome Structure Influences Recombination .” 2015. Web. 13 Dec 2019.

Vancouver:

Mainiero S. Understanding How Chromosome Structure Influences Recombination . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/40718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mainiero S. Understanding How Chromosome Structure Influences Recombination . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

4. Zhou, Adele. Understanding regulation of meiosis in Arabidopsis and maize .

Degree: 2018, Cornell University

URL: http://hdl.handle.net/1813/59496

► Meiosis is a specialized cell division that is required for sexual reproduction. Homologous chromosome pairing and meiotic recombination are two defining processes of prophase I… (more)

▼ Meiosis is a specialized cell division that is required for sexual reproduction. Homologous chromosome pairing and meiotic recombination are two defining processes of prophase I of meiosis and are required for the generation of genetic variation. Core proteins involved in these processes are well studied, but it is not well understood how meiosis is regulated. In this dissertation, I shed light on how meiosis is regulated by long non-coding RNAs (lncRNAs) in Arabidopsis thaliana, as well as how meiotic recombination is regulated by chromatin in maize. Many long non-coding RNAs (lncRNAs) have been identified in various species but few have been functionally analyzed. Of the few lncRNAs that have been analyzed, they have been found to play roles in regulating gene expression and modifying chromatin. However, the functions of the vast majority of them are unknown and no lncRNAs have been identified in Arabidopsis meiosis. On the other hand, significantly more genes are expressed during meiosis than the number of genes actually required for meiosis completion, suggesting that there is a mechanism controlling gene expression post-transcriptionally, possibly involving lncRNAs. This situation prompted us to conduct a systematic analysis of lncRNAs in Arabidopsis meiosis. We generated a bioinformatics pipeline that utilizes RNA-seq data from Arabidopsis meiocytes and somatic tissues to identify novel lncRNAs that are specific to meiosis. We then established a multistep mutant screen to identify lncRNAs that are functional in meiosis and found that lncRNAs play a role in overall fertility in Arabidopsis thaliana. Chapter 3 of this dissertation focuses on understanding the role of chromatin in the regulation of meiotic recombination events that result in crossovers (COs) in maize. In this species, double strand breaks (DSBs) are evenly distributed along chromosomes, including pericentromeric and centromeric regions. However, CO distribution does not follow the DSB distribution. Instead, COs are enriched in distal chromosome regions and suppressed in pericentromeric and centromeric regions. We investigated the dynamics of recombination events in relationship to chromatin states in order to understand how this distribution is formed. We further studied the CO/non-crossover (NCO) decision through its relationship with chromatin. We found that DNA methylation is a major contributor to the decision of which DSBs become COs and this decision is progressively enforced when meiotic DSBs are repaired. Interestingly, this process proceeds differently in B73 and CML228, two maize inbreds with dramatically differing numbers of recombination events. Advisors/Committee Members: Cohen, Paula (committeeMember), Richards, Eric Jean (committeeMember).

Subjects/Keywords: Genetics; meiosis; Molecular biology; crossovers; double strand breaks; epigenetic marks; non-coding RNAs; recombination; Plant sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, A. (2018). Understanding regulation of meiosis in Arabidopsis and maize . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/59496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Adele. “Understanding regulation of meiosis in Arabidopsis and maize .” 2018. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/59496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Adele. “Understanding regulation of meiosis in Arabidopsis and maize .” 2018. Web. 13 Dec 2019.

Vancouver:

Zhou A. Understanding regulation of meiosis in Arabidopsis and maize . [Internet] [Thesis]. Cornell University; 2018. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/59496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou A. Understanding regulation of meiosis in Arabidopsis and maize . [Thesis]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

5. Raghavan, Vandana. INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST .

Degree: 2019, Cornell University

URL: http://hdl.handle.net/1813/67771

► The mismatch repair (MMR) pathway maintains genome stability by repairing mutations incorporated in the genome during replication and recombination. While most microorganisms tend to have… (more)

▼ The mismatch repair (MMR) pathway maintains genome stability by repairing mutations incorporated in the genome during replication and recombination. While most microorganisms tend to have low mutation rates, a higher mutation rate can provide transient adaptive advantage to stress conditions by promoting adaptive mutations. Variants of the MMR genes, MLH1 and PMS1 from different yeast strains can display an incompatibility that results in a high mutation rate. MLH1 and PMS1 function as a heterodimer and the incompatibility is a result of single amino acid polymorphism in each protein. The incompatibility provides an adaptive advantage under stress but does so at the cost of long-term fitness. I identified 18 baker’s yeast isolates from 1011 yeast isolates surveyed that contain the incompatible MLH1-PMS1 genotype in a heterozygous state. I tested the mutation rates of two clinical heterozygous diploid isolates, YJS5885 and YJS5845, and their spore clones. While both of these isolates were non-mutators, their meiotic spore progeny displayed mutation rates that varied over a 340-fold range, and MLH1-PMS1 incompatibility was the major driver of high mutation rate. The range in mutation rates might be in part because these isolates are heterozygous for several genes that may be modifying the mutation rate. My data are consistent with the variance in mutation rate contributing to adaptation to stress conditions through the acquisition of beneficial mutations, with high mutation rates leading to long-term fitness costs that are buffered by mating, or eliminated through natural selection. Furthermore, I observed that one of the isolates was aneuploid and generated aneuploid spore clones at a high frequency. Aneuploidy also provides a transient adaptive advantage under stress conditions. Thus, I obtained evidence for mechanisms in clinical yeast isolates that may provide an adaptive advantage in the human body. Advisors/Committee Members: Peters, Joseph E. (committeeMember), Cohen, Paula (committeeMember).

Subjects/Keywords: Molecular biology; Genetics; Biochemistry; Adaptation; mismatch repair; baker's yeast; clinical isolates; genetic incompatibility; Saccharomyces cerevisiae

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APA (6^th Edition):

Raghavan, V. (2019). INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/67771

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Raghavan, Vandana. “INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST .” 2019. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/67771.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Raghavan, Vandana. “INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST .” 2019. Web. 13 Dec 2019.

Vancouver:

Raghavan V. INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST . [Internet] [Thesis]. Cornell University; 2019. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/67771.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Raghavan V. INCOMPATIBILITIES IN MISMATCH REPAIR GENES MLH1-PMS1 CONTRIBUTE TO A WIDE RANGE OF MUTATION RATES IN HUMAN ISOLATES OF BAKER'S YEAST . [Thesis]. Cornell University; 2019. Available from: http://hdl.handle.net/1813/67771

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

6. Rinaldi, Vera Da Silva Garcia. Genetically dissecting the meiotic checkpoint active during prophase I in female mice .

Degree: 2017, Cornell University

URL: http://hdl.handle.net/1813/56716

► Females have a non-renewable number of gametes at birth. These oocytes are extremely sensitive to environmental factors that generate DNA damage. Oocyte death due to… (more)

▼ Females have a non-renewable number of gametes at birth. These oocytes are extremely sensitive to environmental factors that generate DNA damage. Oocyte death due to DNA damage can result in infertility and ovarian failure. In contrast to postnatal oocytes, at earlier stages of gametogenesis these cells withstand hundreds of developmentally programmed DNA breaks (DSBs). During the first meiotic division these DSBs promote synapsis (homologous chromosomes pairing), and recombination, which are both essential for sexual reproduction and environmental fitness. However, DSB repair and synapsis need to occur in a timely manner, or the quality of the gametes becomes compromised. The mechanisms that guarantee oocyte quality were hypothesized to operate through two independent pathways: one that surveys DNA integrity, and the other synapsis. However, I present experimental evidence that oocytes defective for either DNA repair or synapsis are eliminated by the same DNA damage response. Furthermore, through the detailed analysis of DNA repair dynamics, I provide evidence that the protein HORMAD2, which localizes to unsynapsed chromosomes, regulates DSB-repair. I hypothesize that HORMAD2 interferes with repair by preventing broken DNA from using the sister chromatid as a repair template. This “block to sister-chromatid repair” (BSCR) assures that the homologous chromosome is the substrate of choice. Whereas BSCR guarantees homologous recombination, it also prevents unsynapsed chromosomes from fixing DSBs. Thus, failure to synapse will result in persistent DSBs. Since DNA damage causes oocyte death postnatally, unsynapsed chromosome will trigger the DNA damage checkpoint. Through the understanding of this checkpoint, I was able to test if the transient inhibition of the DNA damage checkpoint protein (CHK2) prevents oocyte death. My finding that oocyte death was prevented, and fertility was preserved, provides evidence that chemically protecting oocyte from DNA damaging agents is a viable clinical approach. This result will hopefully translate into a treatment to delay ovarian failure. Taken together these results have implications on our current understanding of the prophase I checkpoint. TEACHING AS RESEARCH My interest in improving teaching strategies led me to research the qualitative outcome of using a novel teaching tool during the laboratory section of a histology course. I tested an interactive response system (IRS) as formative assessment tool. I found that IRS results in a positive experience, however my study was not able to detect quantitative difference on students’ grades was detected. Advisors/Committee Members: Coonrod, Scott A. (committeeMember), Alani, Eric E. (committeeMember), Cohen, Paula (committeeMember).

Subjects/Keywords: teaching; Developmental biology; meiosis; Biology; Genetics; reproduction; DNA-damage checkpoint; fertility; gametogenesis

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APA (6^th Edition):

Rinaldi, V. D. S. G. (2017). Genetically dissecting the meiotic checkpoint active during prophase I in female mice . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/56716

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rinaldi, Vera Da Silva Garcia. “Genetically dissecting the meiotic checkpoint active during prophase I in female mice .” 2017. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/56716.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rinaldi, Vera Da Silva Garcia. “Genetically dissecting the meiotic checkpoint active during prophase I in female mice .” 2017. Web. 13 Dec 2019.

Vancouver:

Rinaldi VDSG. Genetically dissecting the meiotic checkpoint active during prophase I in female mice . [Internet] [Thesis]. Cornell University; 2017. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/56716.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rinaldi VDSG. Genetically dissecting the meiotic checkpoint active during prophase I in female mice . [Thesis]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/56716

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

7. Cheong, Soon Hon. Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows .

Degree: 2012, Cornell University

URL: http://hdl.handle.net/1813/31001

► Reproductive performance is paramount to efficient milk production in dairy cows. Many postpartum cows do not clear uterine inflammation and resume normal ovarian cyclicity by… (more)

▼ Reproductive performance is paramount to efficient milk production in dairy cows. Many postpartum cows do not clear uterine inflammation and resume normal ovarian cyclicity by the time they are presented for reinsemination. This dissertation aimed to study of factors associated with the delay in clearance of uterine inflammation and resumption of ovarian cyclicity in the postpartum cow. Subclinical endometritis (SCE) is the presence of inflammation in the uterus beyond the normal involution without any other signs of disease. A large epidemiological study was performed obtaining uterine samples, risk factors and reproductive outcome data from 38 commercial dairy herds. Cow-level risk factors for SCE identified were: ketosis, milk production and metritis. Reagent strip test was evaluated as a potential cow-side test for SCE. Reagent strip tests were found to be strongly associated with SCE, however low sensitivity and specificity limits its potential use. The effects uterine sample collections on economically important outcomes on sampled cows were tested and no detrimental effects were found. The main difference between early postpartum follicles that will ovulate and those that do is the ability to produce a rise in circulating estradiol concentrations. A novel follicle fate prediction method was used to identify cows that will likely go on to ovulate or not using follicle growth parameters and circulating estradiol concentrations. This allowed for follicular fluid collection and steroid hormone analyses. In non-ovulatory cows; the theca cell function was impaired, there were fewer luteinizing hormone pulses, and had more severe negative energy balance primarily due to decreased feed intake compared with ovulatory cows. Uterine health association with follicular function was evaluated. Certain bacterial isolates were associated with reduced follicle growth. Follicular fluid endotoxin levels were found to be higher in non-ovulatory cow and they also had higher circulating haptoglobin levels which are an indicator for acute phase response. Negative energy balance and uterine health disorders were associated with both SCE and non-ovulation. Advisors/Committee Members: Roberson, Mark Stephen (committeeMember), Nydam, Daryl Van (committeeMember), Cohen, Paula (committeeMember).

Subjects/Keywords: Dairy Cow; Ovarian cyclicity; Uterine health

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cheong, S. H. (2012). Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/31001

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheong, Soon Hon. “Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows .” 2012. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/31001.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheong, Soon Hon. “Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows .” 2012. Web. 13 Dec 2019.

Vancouver:

Cheong SH. Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows . [Internet] [Thesis]. Cornell University; 2012. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/31001.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheong SH. Factors Associated With The Clearance Of Uterine Inflammation And Resumption Of Ovarian Cyclicity In Postpartum Dairy Cows . [Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31001

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

8. Bozza, Christopher. Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs .

Degree: 2012, Cornell University

URL: http://hdl.handle.net/1813/31407

► Homologous pairing during meiosis is an important process for the fidelity of recombination and chromosome segregation. The activity of homologous pairing has been linked to… (more)

▼ Homologous pairing during meiosis is an important process for the fidelity of recombination and chromosome segregation. The activity of homologous pairing has been linked to events that occur during the early stages of recombination, namely double strand break formation and single end invasion. The exact activities that regulate the genetic network of homologous pairing are not completely understood. The first chapter of this dissertation provides a review of research regarding homologous pairing. To understand more about the activity, a forward genetics approach was used to identify two mutants of maize, segII and dsyCS. Cytological characterization of these mutants and mapping the genetic lesion underlying each of their phenotypes comprise the second and third chapters. Combining FISH and immunolocalization studies, the segII mutant displays abnormalities beginning at the leptotene stage of meiosis, which result in reduced installation of DSB repair proteins, non-homologous pairing, reduced chiasmata, and crossovers between non-homologous chromosomes. Application of cisplatin to segII mutants to induce double strand breaks at leptotene partially rescues repair protein installation, suggesting that a lack of DSBs underlies the abnormal meiotic phenotype of segII. Analysis of the segII mutant also indicates that maize displays crossover homeostasis through its reduced single end invasion events. The number of chiasmata is disproportionally reduced when compared to the number of SEI: 26% versus 2% respectively. The dsyCS mutant was also analyzed using FISH and immunolocazliation studies. It displays a similar repair protein abnormality to segII, a more severe pairing phenotype than segII and, most notably, a high rate of anaphase bridges. A FISH probe to the telomeres reveals interchromosomal connections at diakinesis, which suggests that improper telomere repair is at least partially responsible for the bridges. dsyCS displays non-homologous crossovers, and together with segII and the previously characterized zmRad51 mutant, suggests that maize has at least three genetic paths to create nonhomologous chiasmata. Advisors/Committee Members: Cohen, Paula (committeeMember), Rose, Jocelyn (committeeMember), Scanlon, Michael J. (committeeMember).

Subjects/Keywords: Meiosis; Homologous Pairing; chiasmata; recombination; segII; dsyCS; sei; Crossover homeostasis

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APA (6^th Edition):

Bozza, C. (2012). Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/31407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bozza, Christopher. “Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs .” 2012. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/31407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bozza, Christopher. “Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs .” 2012. Web. 13 Dec 2019.

Vancouver:

Bozza C. Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs . [Internet] [Thesis]. Cornell University; 2012. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/31407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bozza C. Mapping And Cytological Characterization Of Maize Meiotic Mutants Segii And Dsycs . [Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

9. Trevino, Lindsey. Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease .

Degree: 2011, Cornell University

URL: http://hdl.handle.net/1813/33567

► Ovarian cancer is the leading cause of reproductive cancer death in U.S. women. This high mortality rate is due, in part, to the lack of… (more)

▼ Ovarian cancer is the leading cause of reproductive cancer death in U.S. women. This high mortality rate is due, in part, to the lack of early detection methods and incomplete understanding of the origin of the disease. Animal models of ovarian cancer can shed light on the genetic and biological factors that influence tumor development and/or progression, as well as identify strategies for prevention, early detection and treatment. One animal model, the domestic hen, has a high spontaneous incidence of the disease that is age-dependent, similar to women. Although previous studies utilizing the hen as a model for ovarian cancer have characterized chicken ovarian tumors and tested putative prevention and treatment strategies of the disease, our understanding of the development and progression of ovarian cancer in the hen is still limited. Our objectives were 1) to further characterize chicken ovarian tumors through global gene expression analysis; 2) to test the effect of progestin and estrogen together, as commonly delivered in "the pill", as well as progestin and estrogen alone on ovarian cancer prevalence in the hen; and 3) to determine estrogen receptor subtype expression in chicken ovarian tumors. The second and third objectives were based on evidence in women that steroid hormones play a role in ovarian cancer, with estrogen associated with an increased risk and progesterone associated with a decreased risk of the disease. We have shown that administration of "the pill" is associated with a significant decrease in ovarian cancer prevalence, as well as egg production, suggesting that ovulation is important for the initiation of ovarian cancer. Furthermore, we observed that chicken ovarian tumors over-express oviduct-related genes, even at early stages, providing evidence that tumors possibly arise from the epithelial cells of the oviduct. Finally, our results also support a role for steroid hormones, particularly estrogen, in mediating ovarian tumor progression. Collectively, our studies have provided information regarding the development and progression of ovarian cancer in the hen that may help unlock the mysteries of the disease in women. Advisors/Committee Members: Roberson, Mark Stephen (committeeMember), Cohen, Paula (committeeMember), Travis, Alexander J. (committeeMember).

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Trevino, L. (2011). Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/33567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Trevino, Lindsey. “Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease .” 2011. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/33567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Trevino, Lindsey. “Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease .” 2011. Web. 13 Dec 2019.

Vancouver:

Trevino L. Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease . [Internet] [Thesis]. Cornell University; 2011. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/33567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trevino L. Development And Progression Of Ovarian Cancer: Insights From The Hen Model Of The Disease . [Thesis]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/33567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

10. Chuang, Chen-hua. Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals .

Degree: 2012, Cornell University

URL: http://hdl.handle.net/1813/29249

► DNA replication is a fundamental process in all organisms. Using single stranded DNA (ssDNA) as a template, DNA polymerase synthesizes new DNA to produce an… (more)

▼ DNA replication is a fundamental process in all organisms. Using single stranded DNA (ssDNA) as a template, DNA polymerase synthesizes new DNA to produce an exact copy of the genome. However, most DNA polymerases lack the ability to unwind the double stranded DNA (dsDNA) necessary to expose ssDNA [1]. Therefore, an additional DNA helicase is required to initiate DNA replication by unwinding dsDNA and exposure of ssDNA as a template. In eukaryotes, genetic and biochemical assays have shown the MCM (minichromosome maintenance) family of proteins functions in a hexameric complex as the genomic DNA replication helicase [2]. MCMs have been well studied through in vitro biochemistry, cells in culture, and simple model organisms including S. cerevisiea and Xenopus laevis. These experimentally tractable systems have shown that misrelated or dysfunctional MCMs have deleterious consequences, especially genomic instability (GIN). However, there are still several major unresolved issues that need to be addressed. (1) The "MCM paradox" described that the MCMs are in excess of the number of origins. What is the function of these excess MCMs, and how does it relate to cells and animals? (2) The "MCM puzzle" indicates that mini-MCM complexes exist, but their functions are still unclear (3) Very little is known about the function of the MCM helicase with respect to the health of whole animals. To accomplish these issues and explore the relationship between MCMs and disease, I generated mice deficient in MCMs as a model of in vivo disease in this thesis. The mice which carry 50% reduction of Mcm2, 3, 4, 6, and 7 is phenotypically identical to wild-type at least through 1 year of age. Further reduction of Mcms to 70% causes several detrimental phenotypes, including embryonic lethality, growth retardation, genomic instability, and cancer susceptibility. Most importantly, the reduction of MCM3 rescues most of the detrimental phenotypes in other MCM deficient mice, suggesting a unique function of MCM3. Highly similar to in vitro results, I showed that the MCM3/5 dimer inhibits the MCM2-7 complex from binding chromatin and hinders cell cycle. I also discovered that the Mcm4Chaos3 mutation induces a pan-downregulation of Mcm2-7 post-transcriptionally. The pan-down regulation of Mcm2-7 is a self-preservation mechanism because it reduces MCM3 levels that block the recruitment of chromatin bound MCM2-7. Finally, I identified that Mcm hypomorphic mice possess a unique gender bias phenotype. The male animals are more resistant to MCM insufficiency due to a testosterone protective effect. In summary, this dissertation explores the function of the excess MCMs in aspects of cell cycle and in whole animals. It builds understanding about the regulation of MCMs with emphasis upon cancer formation as a result of MCM deficiency. The MCM hypomorphic mice also reveal a post-transcriptional regulation of Mcms that responded to helicase complex instability or insufficiency. The unique negative function of the MCM3/5 dimer overturns the current theory that… Advisors/Committee Members: Weiss, Robert S. (committeeMember), Tye, Bik-Kwoon (committeeMember), Cohen, Paula (committeeMember).

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chuang, C. (2012). Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/29249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chuang, Chen-hua. “Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals .” 2012. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/29249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chuang, Chen-hua. “Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals .” 2012. Web. 13 Dec 2019.

Vancouver:

Chuang C. Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals . [Internet] [Thesis]. Cornell University; 2012. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/29249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chuang C. Unique Regulatory Mechanisms For Dna Replication And Genome Maintenance In Mammals . [Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/29249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

11. Sones, Jenny. Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/39356

► Preeclampsia (PE) is a devastating disorder of pregnancy that affects 10% of pregnancies worldwide. While it is a leading cause of perinatal morbidity and mortality,… (more)

▼ Preeclampsia (PE) is a devastating disorder of pregnancy that affects 10% of pregnancies worldwide. While it is a leading cause of perinatal morbidity and mortality, the cause of PE is unknown. To date the only definitive treatment is delivery of the placenta and the baby, which is often preterm and/or growth restricted. The origins of PE are generally regarded to be early in pregnancy at the time of placenta formation as placental developmental abnormalities often characterize PE pregnancies. In these thesis studies, early pregnancy events required for proper placentation, implantation and decidualization, were investigated in the BPH/5 spontaneous mouse model of PE. We discovered profound developmental defects in implantation, decidualization, and placentation in BPH/5 mice. Along with evidence of decidual hypoxia, these defects were associated with a distinct molecular signature of aberrant embryo-uterine interactions. We identified significant dysregulation in the periimplantation period, particularly in the overexpression of cyclooxygenase 2 (Cox2) in the maternal uterine environment. Using pharmacological intervention at early pregnancy with a selective Cox2 inhibitor, we confirmed that defects in Cox2 signaling during the periimplantation period have "ripple effects" impacting downstream adverse pregnancy outcomes at mid and late gestation in BPH/5 mice, including fetal growth restriction and demise. Finally, investigations in the periimplantation period lead us to discover deficiencies in immunoregulatory pathways crucial for placental development at the maternal-fetal interface in BPH/5 mice. We showed dramatic loss of decidual Natural Killer (dNK) cells and this was associated with overexpression of IL-15 in BPH/5 implantation sites. Furthermore, we linked Cox2 inhibition with a reduction in IL-15. Altogether, this points towards inflammation in the maternal uterine environment during early pregnancy having a central role in the poor fetoplacental and pregnancy outcomes associated with this model and highlights Cox2 inhibitors as potential therapeutics to prevent fetal morbidity/mortality associated with PE. Importantly, our data supports the "ripple effect" hypothesis that defects in early pregnancy events are the source of downstream adverse pregnancy outcomes using the BPH/5 mouse model of PE. Advisors/Committee Members: Cohen, Paula (committeeMember), Roberson, Mark Stephen (committeeMember), Quirk, Susan Mary (committeeMember).

Subjects/Keywords: preeclampsia; pregnancy; placenta

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sones, J. (2015). Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/39356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sones, Jenny. “Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia .” 2015. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/39356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sones, Jenny. “Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia .” 2015. Web. 13 Dec 2019.

Vancouver:

Sones J. Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/39356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sones J. Defects In Periimplantation Signaling Contribute To Adverse Pregnancy Outcomes In The Bph/5 Mouse Model Of Preeclampsia . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/39356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

12. Stephens, Claire. Follicle Selection And Growth In The Domestic Hen Ovary .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/40897

► Ovarian follicle selection and development in domestic hens is highly regulated. A single small follicle (approximately 6-8 mm in diameter) is selected daily and enters… (more)

▼ Ovarian follicle selection and development in domestic hens is highly regulated. A single small follicle (approximately 6-8 mm in diameter) is selected daily and enters the pre-ovulatory hierarchy, continuing to grow until ovulation occurs. We investigated the mechanism(s) involved in follicle selection and subsequent growth before ovulation. In the first study, we found bone morphogenetic protein 15 (BMP15) mRNA primarily in the oocyte, and BMP15 protein was detected in 6 and 8 mm follicles around the time of follicle selection. In addition, BMP15 altered mRNA expression of genes associated with follicle selection. Granulosa cell culture with BMP15 increased mRNA levels of follicle stimulating hormone receptor (FSHR) and decreased both anti-Müllerian hormone (AMH) and occludin (OCLN). OCLN protein, a tight junction protein, also decreased, suggesting that this oocyte factor may play a pivotal role in follicle selection into the pre-ovulatory hierarchy. Liver-derived yolk material enters follicles via capillaries in the theca layer, ultimately reaching oocytes via paracellular spaces between granulosa cells. Yolk is incorporated into the oocyte by receptor-mediated endocytosis. OCLN may regulate access of yolk material to the oocyte surface. We determined expression and regulation of OCLN in small follicles. OCLN mRNA expression decreased with increasing follicle size. The regulation of OCLN transcripts in laying hens was investigated by granulosa cell culture. BMP15, epidermal growth factor (EGF) and activin B decreased OCLN expression, whereas activin A increased OCLN mRNA, suggesting local factors may regulate yolk access to the oocyte. Broiler breeder hens have aberrant follicle development that is modulated by limiting feed intake. Restricted diets produce more ordered follicle development and yolk uptake, but the causative factors are unknown. We examined metabolic-related factors in the liver and global gene expression to identify altered transcripts that contribute to abnormal follicular growth. The GH/IGF1-axis may be overstimulated in hens on an ad libitum diet and feed intake alters genes involved in lipid metabolism. These studies offer new insights into specific intra-ovarian and extra-ovarian factors mediating follicle selection and growth in the hen. Advisors/Committee Members: Roberson,Mark Stephen (committeeMember), Cohen,Paula (committeeMember), Fortune,Joanne Elizabeth (committeeMember).

Subjects/Keywords: ovary; follicle development; hen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stephens, C. (2015). Follicle Selection And Growth In The Domestic Hen Ovary . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40897

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stephens, Claire. “Follicle Selection And Growth In The Domestic Hen Ovary .” 2015. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/40897.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stephens, Claire. “Follicle Selection And Growth In The Domestic Hen Ovary .” 2015. Web. 13 Dec 2019.

Vancouver:

Stephens C. Follicle Selection And Growth In The Domestic Hen Ovary . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/40897.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stephens C. Follicle Selection And Growth In The Domestic Hen Ovary . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40897

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

13. Cooper, Lara. Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue.

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/44336

► Assisted reproductive techniques, including in vitro development of follicles, cryopreservation and in vitro fertilization, are used to preserve female fertility. Ovarian tissue cryopreservation is a… (more)

▼ Assisted reproductive techniques, including in vitro development of follicles, cryopreservation and in vitro fertilization, are used to preserve female fertility. Ovarian tissue cryopreservation is a promising tool for maintaining fertility in women undergoing cancer treatment and for gamete rescue in wildlife species. Primordial follicles, the basic units of folliculogenesis that represent a female's reproductive potential, should be the priority when preserving fertility. Thus, a thorough understanding of primordial follicle activation, the first step of folliculogenesis, is paramount. The aims of this dissertation were to advance understanding of primordial follicle activation, examine the effects of ovarian tissue cryopreservation on activation, and identify the molecular impact of cryopreservation on follicular health and function. In a fetal bovine model, bone morphogenetic protein 4 (BMP4) stimulated primordial follicle activation in cultured ovarian tissue. Co-culture of ovarian issue with BMP4 and a blocker of KIT suggested that BMP4 stimulates activation by regulating the KIT ligand/KIT pathway. Analysis by qPCR, however, revealed that BMP4 did not regulate expression of mRNA for KIT ligand or KIT. Cryopreservation of fetal bovine ovarian tissue using needle immersion vitrification demonstrated that exposure to cryoprotectants and ultra-fast cooling did not impact primordial follicle activation. Furthermore, though the ovarian tissue sustained some damage following exposure to cryoprotectants and vitrification, normal follicular morphology was recovered after seven days of culture. The addition of sucrose to the cryoprotectants, however, did not improve follicular morphology. The protocol for needle immersion vitrification was optimized for peri-pubertal feline ovarian tissue. Cryoprotectant exposure temperature affected follicular health and function, with 4[MASCULINE ORDINAL INDICATOR]C being preferable to room temperature. However, the damage sustained due to vitrification was not repaired during culture, as it was in the fetal bovine tissue. Once again, sucrose did not improve follicular health or function. Nascent RNA transcription was found to be an early marker of follicular health. These new findings for primordial follicle activation and ovarian tissue vitrification can be used to improve assisted reproductive techniques, thereby advancing fertility preservation in women and wildlife species. Advisors/Committee Members: Comizzoli,Pierre (committeeMember), Cohen,Paula (committeeMember), Travis,Alexander J. (committeeMember).

Subjects/Keywords: Ovary; Primordial follicle activation; Cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cooper, L. (2016). Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/44336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cooper, Lara. “Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue. ” 2016. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/44336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cooper, Lara. “Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue. ” 2016. Web. 13 Dec 2019.

Vancouver:

Cooper L. Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue. [Internet] [Thesis]. Cornell University; 2016. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/44336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cooper L. Activation And Survival Of Ovarian Follicles In Vitro: The Effects Of Bone Morphogenetic Proteins (Bmps) And/Or Cryopreservation On Bovine And Feline Ovarian Tissue. [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/44336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

14. Chu, Erin Tsi-Jia. MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES .

Degree: 2017, Cornell University

URL: http://hdl.handle.net/1813/59013

► Epigenetic modifications are known to regulate gene expression in a heritable manner, and can broadly be divided into three interacting classes: DNA methylation, histone modifications,… (more)

▼ Epigenetic modifications are known to regulate gene expression in a heritable manner, and can broadly be divided into three interacting classes: DNA methylation, histone modifications, and chromatin interactions. While the trans acting factors that establish, maintain, and remove DNA methylation are well-known, the cis acting mechanisms that direct DNA methylation to specific genomic locations remain elusive. Two gene classes offer insights into cis-acting mechanisms for DNA methylation: imprinted loci, and transposable elements. A locus spanning both is the murine Rasgrf1 locus. Rasgrf1 has a cis element, a series of tandem repeats, required for DNA methylation, but also harbors a long noncoding RNA, the pitRNA. The pitRNA is driven by the repeats and is targeted by piRNAs, small RNAs that mediate transposable element methylation in the mammalian male germline. However, the effects of the pitRNA versus the repeats have not yet been separated. My work, where I used CRISPR/Cas9 genome editing to generate a targeted mutant system permitting inducible control of the pitRNA, is the first to query the sufficiency of the pitRNA independently. Using quantitative qPCR and targeted bisulfite sequencing, I demonstrated that expression of the lncRNA at physiological levels in the male germline is insufficient to impart DNA methylation at Rasgrf1. These findings were complimented by additional in vitro studies, where I identified Sp1 as a transcription factor that binds the repeats and is required for pitRNA expression. Sp1 binds secondary DNA structure and has recently been identified as a regulating factor at another imprinted gene. Together, these findings support an alternative, critical role for the repeats beyond their known role in regulating pitRNA expression. Beyond mechanism, DNA methylation in the context of disease are an area of active study, though its utility in non-traditional model organisms is nascent. The second focus of my thesis speaks to this. I performed reduced representation bisulfite analysis on two dog breeds with highly diverse morphology and disease risks. While this work is largely preliminary, two differentially methylated regions have direct association with differential disease risk between these two breeds, suggesting that the canine methylome could be used as method of disease surveillance. Advisors/Committee Members: Schimenti, John C. (committeeMember), Cohen, Paula (committeeMember), Kurpios, Natasza (committeeMember).

Subjects/Keywords: Genetics; Molecular biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chu, E. T. (2017). MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/59013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chu, Erin Tsi-Jia. “MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES .” 2017. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/59013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chu, Erin Tsi-Jia. “MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES .” 2017. Web. 13 Dec 2019.

Vancouver:

Chu ET. MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES . [Internet] [Thesis]. Cornell University; 2017. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/59013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chu ET. MECHANISMS AND CONSEQUENCES OF DNA METHYLATION IN TWO MODEL SPECIES . [Thesis]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/59013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. White, Simone Lenora. Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster .

Degree: 2017, Cornell University

URL: http://hdl.handle.net/1813/51676

► The process of fertilization involves many interactions between males, females, and their gametes. This is even more complex in cases of multiple mating, such as… (more)

Subjects/Keywords: Genetics

12 more images…

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APA (6^th Edition):

White, S. L. (2017). Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/51676

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

White, Simone Lenora. “Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster .” 2017. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/51676.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

White, Simone Lenora. “Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster .” 2017. Web. 13 Dec 2019.

Vancouver:

White SL. Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster . [Internet] [Thesis]. Cornell University; 2017. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/51676.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

White SL. Investigating the Female's Role in Sperm Competition in Drosophila Melanogaster . [Thesis]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/51676

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

16. Taylor, David. Dna Methylation: Cis, Trans, And Intergenerational .

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/45164

Subjects/Keywords: Epigenetics; DNA methylation; Intergenerational effects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Taylor, D. (2016). Dna Methylation: Cis, Trans, And Intergenerational . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/45164

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Taylor, David. “Dna Methylation: Cis, Trans, And Intergenerational .” 2016. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/45164.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Taylor, David. “Dna Methylation: Cis, Trans, And Intergenerational .” 2016. Web. 13 Dec 2019.

Vancouver:

Taylor D. Dna Methylation: Cis, Trans, And Intergenerational . [Internet] [Thesis]. Cornell University; 2016. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/45164.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Taylor D. Dna Methylation: Cis, Trans, And Intergenerational . [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/45164

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

17. Hilz, Stephanie Renee. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling.

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/45350

Subjects/Keywords: microRNAs; spermatogenesis; meiosis

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APA (6^th Edition):

Hilz, S. R. (2016). Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/45350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hilz, Stephanie Renee. “Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. ” 2016. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/45350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hilz, Stephanie Renee. “Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. ” 2016. Web. 13 Dec 2019.

Vancouver:

Hilz SR. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. [Internet] [Thesis]. Cornell University; 2016. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/45350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hilz SR. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/45350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

18. Mleczko, Joanna. The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks .

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/45223

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APA (6^th Edition):

Mleczko, J. (2016). The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/45223

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mleczko, Joanna. “The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks .” 2016. Thesis, Cornell University. Accessed December 13, 2019. http://hdl.handle.net/1813/45223.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mleczko, Joanna. “The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks .” 2016. Web. 13 Dec 2019.

Vancouver:

Mleczko J. The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks . [Internet] [Thesis]. Cornell University; 2016. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1813/45223.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mleczko J. The 9-1-1 Dna Damage Response Complex Coordinates Fanconi Anemia And Homologous Recombination Proteins In Responding To Interstrand Cross-Links And Dna Breaks . [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/45223

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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