+publisher:"Colorado State University" +contributor:("Rhyan, Jack")

Colorado State University

1. Herman, Julia Anne. Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment.

Degree: MS(M.S.), Clinical Sciences, 2013, Colorado State University

URL: http://hdl.handle.net/10217/79061

► As a nationally recognized symbol of perseverance and survival, the American bison (Bison bison) continues to be the focus of many conservation efforts. This is… (more)

▼ As a nationally recognized symbol of perseverance and survival, the American bison (Bison bison) continues to be the focus of many conservation efforts. This is particularly true in Yellowstone National Park (YNP) where genetic diversity and population dynamics are threatened by the presence of Brucella abortus infection, the causative agent of brucellosis. To further complicate management efforts, the potential for transmission of B. abortus to livestock surrounding the park could have effects on human and animal health in addition to negative economic impacts. Genetic natural resistance to infectious diseases such as brucellosis has been examined in bison and other species. Consideration of using genetic natural resistance as a management tool within YNP relies on its applicability to the population and effectiveness in identifying susceptible or resistant animals. The aim of this study was to further investigate genetic natural resistance to brucellosis in bison using the prion protein gene (PRNP). This could provide a genetic management tool for future populations by identifying resistant and susceptible genotypes to brucellosis that governing agencies could screen when selecting animals for removal. Animals used in this study were part of the Bison Quarantine Feasibility Study (BQFS), which was conducted by state and federal government agencies to explore alternative options for bison that leave the protection of the park's perimeters as part of the Interagency Bison Management Plan (IBMP). After completing the study, the bison involved will be released into areas of their historic range and managed as a conservation herd. In addition to investigating genetic natural resistance, other genetic aspects were analyzed in this quarantine herd. We evaluated genetic diversity at 42 microsatellite loci representing each of the nuclear chromosomes in the bison genome. These markers allowed us to determine parentage which helped quantify the genetic diversity contained in the conservation herd. It was also important to understand the genetic diversity of the herd and to determine if any genetic characteristics such as cattle DNA introgression or low genetic diversity may threaten the protected status of this herd. Previously developed mitochondrial and nuclear DNA microsatellite markers were applied to determine if any introgression of domestic cattle DNA is presen. PRNP examination provided conflicting results from previous bison PRNP studies, justifying further research into the importance of PRNP in genetic natural resistance. As a conservation herd, this group of bison demonstrates high retention of unique YNP genetic diversity, high percentage of adult animals contributing offspring, and no evidence of cattle DNA introgression. This study explains support for using YNP bison as stocking animals for future satellite herds under the condition that BQFS protocol is followed. Results of these genetic analyses provide a thorough description of a quarantined herd of YNP bison destined for release to brucellosis-free public… Advisors/Committee Members: Salman, Mo (advisor), Hill, Ashley (committee member), Huyvaert, Kathryn (committee member), Rhyan, Jack (committee member).

Subjects/Keywords: bison; brucellosis; genetic diversity; PRNP; satellite herd; Yellowstone National Park

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APA (6^th Edition):

Herman, J. A. (2013). Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/79061

Chicago Manual of Style (16^th Edition):

Herman, Julia Anne. “Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment.” 2013. Masters Thesis, Colorado State University. Accessed April 14, 2021. http://hdl.handle.net/10217/79061.

MLA Handbook (7^th Edition):

Herman, Julia Anne. “Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment.” 2013. Web. 14 Apr 2021.

Vancouver:

Herman JA. Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment. [Internet] [Masters thesis]. Colorado State University; 2013. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10217/79061.

Council of Science Editors:

Herman JA. Genetic natural resistance to brucellosis in Yellowstone National Park bison (Bison bison): a preliminary assessment. [Masters Thesis]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/79061

Colorado State University

2. Orahood, Darcy Sonya. Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon.

Degree: MS(M.S.), Clinical Sciences, 2013, Colorado State University

URL: http://hdl.handle.net/10217/81048

► Aquaculture is a rapidly growing industry that significantly contributes to the world food supply. Sustainable practices in aquaculture are of increasing importance as an increasing… (more)

▼ Aquaculture is a rapidly growing industry that significantly contributes to the world food supply. Sustainable practices in aquaculture are of increasing importance as an increasing proportion of fish in the global market come from aquaculture instead of wild catch. Maximizing aquaculture yields and minimizing the ecological impacts of these operations are two important goals towards sustainability. One approach to addressing these objectives is immunocontraception of fish which would increase the fish meat quality and yield in aquaculture production and prevent escaped farmed fish from undesirably altering wild fish population genetics through breeding. The research presented here was conducted with the aim of proof of concept for contraceptive vaccine use in Atlantic salmon. Nine vaccine formulations, including a negative control vaccine, were formulated at the National Wildlife Research Center in Fort Collins, Colorado and injected into farmed Atlantic salmon in Sunndalsøra, Norway. Production of antibodies against three immunogenic components in each vaccine formulation was evaluated over the course of the 12-week study. Weight and length of each fish were also tracked over time to determine whether growth was affected by vaccination. The study results indicate that Atlantic salmon will produce antibodies against BSA and KLH used as carrier proteins but that KLH is a stronger immunogen. Importantly, it was also determined that Atlantic salmon will produce antibodies against a small endogenous peptide (hapten) conjugated to the carrier protein, but to a lesser extent than the levels of antibody production against the carrier itself. Approximately 96% of samples from fish vaccinated against KLH, 76% of samples from fish vaccinated against BSA, and 36% of samples from fish vaccinated against the hapten were identified as positive. Response rates for all three antigens were highest 12 weeks post-vaccination. Significant differences in antibody levels were also detected between groups vaccinated with different immunostimulants. Collectively, the results provide proof of concept and provide a building block for further research on immunocontraception of Atlantic salmon for application in aquaculture. Advisors/Committee Members: Salman, M. D. (advisor), Eckery, Douglas C. (committee member), Miller, Lowell A. (committee member), Myrick, Christopher A. (committee member), Rhyan, Jack C. (committee member).

Subjects/Keywords: vaccine; reproduction; immunocontraception; Atlantic salmon

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APA (6^th Edition):

Orahood, D. S. (2013). Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/81048

Chicago Manual of Style (16^th Edition):

Orahood, Darcy Sonya. “Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon.” 2013. Masters Thesis, Colorado State University. Accessed April 14, 2021. http://hdl.handle.net/10217/81048.

MLA Handbook (7^th Edition):

Orahood, Darcy Sonya. “Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon.” 2013. Web. 14 Apr 2021.

Vancouver:

Orahood DS. Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon. [Internet] [Masters thesis]. Colorado State University; 2013. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10217/81048.

Council of Science Editors:

Orahood DS. Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon. [Masters Thesis]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/81048

Colorado State University

3. Powers, Jenny. Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni).

Degree: PhD, Biomedical Sciences, 2011, Colorado State University

URL: http://hdl.handle.net/10217/48211

To view the abstract, please see the full text of the document. Advisors/Committee Members: Nett, Terry (advisor), Baker, Dan (advisor), Hansen, Thomas (committee member), Rhyan, Jack (committee member), Peel, Kraig (committee member).

Subjects/Keywords: fertility control; wildlife; immunocontraception; GnRH; elk

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APA (6^th Edition):

Powers, J. (2011). Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni). (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/48211

Chicago Manual of Style (16^th Edition):

Powers, Jenny. “Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni).” 2011. Doctoral Dissertation, Colorado State University. Accessed April 14, 2021. http://hdl.handle.net/10217/48211.

MLA Handbook (7^th Edition):

Powers, Jenny. “Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni).” 2011. Web. 14 Apr 2021.

Vancouver:

Powers J. Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni). [Internet] [Doctoral dissertation]. Colorado State University; 2011. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10217/48211.

Council of Science Editors:

Powers J. Reproductive, behavioral, and first generational effects of gonadotropin releasing hormone vaccination in female Rocky Mountain elk (Cervus elaphus nelsoni). [Doctoral Dissertation]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/48211

Colorado State University

4. Kemp, Jeffrey M. Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.

Degree: PhD, Biomedical Sciences, 2011, Colorado State University

URL: http://hdl.handle.net/10217/70445

To view the abstract, please see the full text of the document. Advisors/Committee Members: Graham, James (advisor), Huyvaert, Kathryn P. (committee member), Bowen, Richard (committee member), Miller, Lowell (committee member), Rhyan, Jack (committee member).

Subjects/Keywords: immunocontraception; vaccine stabilization; recombinant bacteria; oral delivery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kemp, J. M. (2011). Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/70445

Chicago Manual of Style (16^th Edition):

Kemp, Jeffrey M. “Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.” 2011. Doctoral Dissertation, Colorado State University. Accessed April 14, 2021. http://hdl.handle.net/10217/70445.

MLA Handbook (7^th Edition):

Kemp, Jeffrey M. “Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.” 2011. Web. 14 Apr 2021.

Vancouver:

Kemp JM. Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. [Internet] [Doctoral dissertation]. Colorado State University; 2011. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10217/70445.

Council of Science Editors:

Kemp JM. Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. [Doctoral Dissertation]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/70445

Colorado State University

5. Nol, Pauline. Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection.

Degree: PhD, Clinical Sciences, 2010, Colorado State University

URL: http://hdl.handle.net/10217/39046

► Wildlife reservoirs of Mycobacterium bovis are believed to play very important roles in the epidemiology of bovine tuberculosis in many countries throughout the world. In… (more)

▼ Wildlife reservoirs of Mycobacterium bovis are believed to play very important roles in the epidemiology of bovine tuberculosis in many countries throughout the world. In the United States, a free-ranging white-tailed deer (Odocoileus virginianus) population in northeastern Michigan serves as such a reservoir. Although changes in management have decreased prevalence of the disease, additional tools, such as vaccination, are needed to achieve eradication of bovine tuberculosis from Michigan deer. In this project, the efficacy of oral and parenteral Mycobacterium bovis bacille Calmette-Guerin Danish strain 1331 (BCG) was evaluated for its ability to protect white-tailed deer against disease caused by M. bovis infection. In addition, cellular and humoral immune responses in deer to BCG vaccination and M. bovis challenge were examined, and molecular detection techniques were developed to monitor shedding of BCG and M. bovis in vaccinated and infected animals. Results indicate that white-tailed deer vaccinated with BCG both orally and parenterally were protected from development of severe disease after experimental infection with virulent M. bovis when compared to unvaccinated deer. Antibody responses to M. bovis antigens by the deer in this study were evaluated over time using multiantigen (multiantigen print immunoassay, rapid test, and immunoblot to whole-cell sonicate) and single antigen tests (lipoarabinomannan [LAM] ELISA and immunoblot to MPB83). Multiantigen tests detected minimal to no antibody responses in vaccinated deer after challenge, whereas antibody responses were more readily detectable by these tests in unvaccinated deer with more advanced disease. The ELISA results indicated an overall decrease in detectable antibodies produced against LAM-enriched mycobacterial antigen in vaccinated animals as compared to unvaccinated animals after challenge. Few trends could be determined from the immunoblot results. Cellular immunity was measured via interferon gamma production and lymphocyte proliferation in response to mycobacterial antigens. Findings in regards to cellular immunity were inconclusive. Molecular techniques developed to detect M. tuberculosis complex in cervid feces, nasal and pharyngeal swabs, soil, feed, and hay produced data indicating that deer shed M. bovis and BCG only intermittently at 1-3 months after vaccination and 1-4 months after M. bovis challenge. The findings from these studies strongly support further research that could lead to the eventual use of BCG in wild white-tailed deer herds affected by bovine tuberculosis. The data also encourage the improvement and potential use of antibody-based assays, such as the multiantigen tests and the LAM-ELISA as ante-mortem tools to assess disease progression in white-tailed deer in both experimental and field vaccine trials. Finally, further improvements in the molecular detection of M. bovis could enable more effective monitoring of shedding of M. bovis by deer in both experimental and free-ranging environments. Advisors/Committee Members: Salman, Mowafak Dauod (advisor), Rhyan, Jack (committee member), Belisle, John Theodore (committee member), Hill, Ashley E. (committee member), Keefe, Thomas J. (committee member).

Subjects/Keywords: Bacille Calmette-Guérin vaccines; wildlife vaccination; Bacille Calmette-Guerin; oral vaccination; bovine tuberculosis; white-tailed deer; White-tailed deer – Tuberculosis; Mycobacterium bovis; Mycobacterium bovis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nol, P. (2010). Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/39046

Chicago Manual of Style (16^th Edition):

Nol, Pauline. “Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection.” 2010. Doctoral Dissertation, Colorado State University. Accessed April 14, 2021. http://hdl.handle.net/10217/39046.

MLA Handbook (7^th Edition):

Nol, Pauline. “Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection.” 2010. Web. 14 Apr 2021.

Vancouver:

Nol P. Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection. [Internet] [Doctoral dissertation]. Colorado State University; 2010. [cited 2021 Apr 14]. Available from: http://hdl.handle.net/10217/39046.

Council of Science Editors:

Nol P. Vaccination of white-tailed deer with Mycobacterium bovis bacille Calmette-Guerin: efficacy, immunology, and molecular detection. [Doctoral Dissertation]. Colorado State University; 2010. Available from: http://hdl.handle.net/10217/39046

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