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You searched for +publisher:"Cape Peninsula University of Technology" +contributor:("Kambizi, L., Prof"). One record found.

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Cape Peninsula University of Technology

1. Mlungwana, Asanda. In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) .

Degree: 2018, Cape Peninsula University of Technology

Drosanthemum micans and Drosanthemum hallii are endangered succulent shrubs of horticultural and medicinal value. They are restricted to the Succulent Karroo, which is one of the world’s biodiversity hotspots. The species risk extinction from illegal over-harvesting for water-wise gardens, erosion by occasional flush floods from ephemeral rivers, competition from alien invasive species, overgrazing and clearing of land for agriculture and human settlement. Although seeds and cuttings may be used in propagating these species, they often require seasonal collection and planting and cuttings struggle to establish, hence the need for in-vitro propagation as an alternative solution. Thus, the main objective of the study was to develop a method for rapid in-vitro shoot and root multiplication and acclimatization of D. micans and D. hallii. To initiate shoot formation, disinfected leaf and stem nodal explants were cultured in Murashige and Skoog (1962) media supplemented with different rates (0, 10, 20 or 30μM) of 2-isopentyladenine, 6-Benzyladenine and kinetin for D. hallii or 2-isopentyladenine, 6-Benzyladenine and Thiadiazuron for D. micans. Shoots from explants were rooted in varying rates (0, 10, 20 or 30μM) of IAA for root initiation. Three media, which were used in previous studies, were tested for acclimatization of rooted explants in i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%). For quantitative evaluation of plant stress, chlorophyll fluorescence index (Fv/Fm) was measured as a proxy for plant stressf stress. It emerged that stem nodal explants of D. hallii tend to produce multiple shoots whilst leaf explants tended to produce callus when cultured in full-strength Murashige and Skoog (1962). Shoot multiplication was optimal in both D. hallii and D. micans at 10 μM of kinetin. Root formation in both D. hallii and D. micans only occurred when shoots were transferred to a full-strength Murashige and Skoog (1962) media without any phytohormones added. The intensity of tissue browning increased at higher levels of cytokinins, suggesting an interaction of plant growth regulators with exudates from explants. Different acclimatization media tested showed no significant differences in the level of stress (Fv/Fm). It is recommended that Murashige and Skoog (1962) media with10 μM kinetin be used for shoot development and multiplication, followed by transfer of the shoots to fresh full-strength Murashige and Skoog (1962) media without hormones for root development. Acclimatization of the rooted explants was possible in one of the following media: i) vermiculite, ii) sand (50%): vermiculite (50%) or iii) sand (75%): perlite (25%) and in a misted greenhouse (ca. 60% RH), with gradual weekly reductions in humidity by 10% over 2 weeks. Advisors/Committee Members: Kambizi, L., Prof (advisor), Matimati, I., Dr (advisor).

Subjects/Keywords: Drosanthemum micans; Drosanthemum hallii; Aizoaceae  – Micropropagation; Plant micropropagation; Plant tissue culture; Plant cell culture; Plants  – Analysis; Plant conservation

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APA (6th Edition):

Mlungwana, A. (2018). In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) . (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/2748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mlungwana, Asanda. “In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) .” 2018. Thesis, Cape Peninsula University of Technology. Accessed November 22, 2019. http://etd.cput.ac.za/handle/20.500.11838/2748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mlungwana, Asanda. “In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) .” 2018. Web. 22 Nov 2019.

Vancouver:

Mlungwana A. In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) . [Internet] [Thesis]. Cape Peninsula University of Technology; 2018. [cited 2019 Nov 22]. Available from: http://etd.cput.ac.za/handle/20.500.11838/2748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mlungwana A. In-vitro propagation studies of the endangered succulents Drosanthemum Micans and Drosanthemum Hallii (Aizoaceae) . [Thesis]. Cape Peninsula University of Technology; 2018. Available from: http://etd.cput.ac.za/handle/20.500.11838/2748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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