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You searched for +publisher:"California State University – San Marcos" +contributor:("Jayasinghe, Sajith"). One record found.

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1. Heisinger, Christopher. Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column .

Degree: 2013, California State University – San Marcos

Glutaraldehyde has been used for decades as an effective crosslinker for proteins. This study explored the effects of glutaraldehyde crosslinking on the efficiency of an Anti-streptolysin O (ASO) purification column. Presently, ASO columns are generally produced by binding streptolysin O (SLO) to the column???s resin. After several elutions with low pH buffers, these columns begin to lose efficacy either by SLO denaturation or by degradation of the covalent bond between SLO and the column. In an effort to increase the lifespan and yield of the ASO purification column set-up, glutaraldehyde was utilized to crosslink the SLO to the resin. Crosslinking with glutaraldehyde was pursued in an attempt to raise the profit margin for ASO produced by column purification. Overall, this study demonstrated that the efficiency of ASO purification columns cannot be improved by crosslinking the SLO-resin complex with glutaraldehyde. However, this study showed the hemolytic activity of SLO is not significantly affected by the presence of inactivated SLO (via glutaraldehyde crosslinking). This ability to form SLO pores in the presence of inactivated SLO may have significant implications for medical treatment of diseases caused by streptococcus infection in a patient???s bloodstream. Comparative gel electrophoresis of SLO samples suggests SLO is the protein present at approximately 60kDa. Previous studies of SLO describe its molecular weight to be between 50 and 75kDa [2]. The results of this study suggest two future directions. First, the findings for the glutaraldehyde crosslinking suggest the exploration of alternative methods for enhancing the stability of SLO on the ASO purification column. Second, the SLO gel electrophoresis results should be confirmed by a western blot analysis utilizing ASO and a fluorescently labeled anti-IgG antibody. Advisors/Committee Members: Jayasinghe, Sajith (advisor).

Subjects/Keywords: Glutaraldehyde Crosslinking; ASO Purification Column; SLO

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Heisinger, C. (2013). Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column . (Thesis). California State University – San Marcos. Retrieved from http://hdl.handle.net/10211.8/420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Heisinger, Christopher. “Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column .” 2013. Thesis, California State University – San Marcos. Accessed December 08, 2019. http://hdl.handle.net/10211.8/420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Heisinger, Christopher. “Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column .” 2013. Web. 08 Dec 2019.

Vancouver:

Heisinger C. Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column . [Internet] [Thesis]. California State University – San Marcos; 2013. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/10211.8/420.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heisinger C. Evaluation of Glutaraldehyde Crosslinking Reactions to Enhance the Efficiency of an Anti-streptolysin O Purification Column . [Thesis]. California State University – San Marcos; 2013. Available from: http://hdl.handle.net/10211.8/420

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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