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You searched for +publisher:"Brown University" +contributor:("Scott, Jeffrey"). Showing records 1 – 2 of 2 total matches.

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1. Budrewicz, Jay. Novel Heart Failure Model Development in Swine.

Degree: Biomedical Engineering, 2017, Brown University

Abstract of Novel Heart Failure Development in Swine, by Jay Budrewicz, ScM., Brown University, May 2017. Objective: There is a need for a simple and reliable large animal doxorubicin cardiotoxicity heart failure model that is created through similar means as it is caused in humans. The model is necessary for the testing of novel and existing drugs and medical devices used to treat this condition. Methods: Two juvenile Yorkshire swine were administered different doses per body mass (30mg/m2 and 3mg/m2) of a doxorubicin hydrogel. It was serially delivered, percutaneously through the sub-xyphoid region, to the pericardial sac. The doxorubicin hydrogel was formulated to become viscous and remain in the pericardium following weekly administrations. Transesophageal Echocardiography Data, hemodynamic data and general health data were collected to evaluate the effects of the toxin. At necropsy evidence of macroscopic findings were collected and processed for histological examination. Results: The animal that received the 30mg/m2 dose displayed clinical observations (dyspnea and wet cough) that prevented it from surviving for more than two administrations or reaching the intended 42 day time point. This animal had a left ventricular ejection fraction that decreased from 56 to 23%. Histological observations of the left atrium showed evidence of fibrous exudates in the pericardium. In the myocardium of the left atrium, there was a zone of superficial myocardial pallor-degeneration. The animal that received the 3mg/m2 dose did not exhibit any signs of doxorubicin induced heart failure. Conclusion: The two doses tested in this doxorubicin induced heart failure model development appear to be too high and two low. The 30mg/m2 dose resulted in off target cardiotoxicity described as pericarditis/epicarditis combined with pericardial effusion resulting in right sided heart failure. The 3mg/m2 did not result in any significant cardiotoxicity. Doses in between the two tested for this model need to be used in an attempt to identify the right dose that will be successful in creating the desired toxicity. Advisors/Committee Members: Mathiowitz, Edith (Advisor), Schell, Jacquelyn (Reader), Scott, Jeffrey (Reader).

Subjects/Keywords: Heart failure

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Budrewicz, J. (2017). Novel Heart Failure Model Development in Swine. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733284/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Budrewicz, Jay. “Novel Heart Failure Model Development in Swine.” 2017. Thesis, Brown University. Accessed March 28, 2020. https://repository.library.brown.edu/studio/item/bdr:733284/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Budrewicz, Jay. “Novel Heart Failure Model Development in Swine.” 2017. Web. 28 Mar 2020.

Vancouver:

Budrewicz J. Novel Heart Failure Model Development in Swine. [Internet] [Thesis]. Brown University; 2017. [cited 2020 Mar 28]. Available from: https://repository.library.brown.edu/studio/item/bdr:733284/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Budrewicz J. Novel Heart Failure Model Development in Swine. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733284/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Vasquez, Stephany. High and Low Compressive Loading on Tissue Engineered Cartilage.

Degree: Biomedical Engineering, 2017, Brown University

Introduction: Osteoarthritis is a debilitating joint disease that affects over 27 million Americans. Tissue engineered cartilage could be a viable option for patients that have lost cartilage due to injury or osteoarthritis. However, reaching optimal mechanical and biochemical properties in tissue engineered cartilage is still a challenge. This study investigates the dose-dependent effects of compressive loading on chondrocyte seeded agarose hydrogels over a 6-week study. Our hypothesis is that early high compressive loading will enhance GAG deposition. Methods: Primary chondrocytes were isolated from porcine knees and digested in 0.15% Collagenase Type II overnight at 37°C. Cells were suspended in 2% agarose and punched into discs with final dimensions was 4 mm diameter and 1.5 mm thick. After two weeks of static culture in chondrogenic media with TGF-β and Dexamethasone, dynamic loading was applied with a 5% tare strain followed by 10% or 30% dynamic compressive loading, or a combination of the two, at 1 Hz for 3hrs/day and 5 days/week for four weeks. The data was analyzed using ANOVA with Tukey Method with a confidence of 95%. Results: On day 28, samples loaded with 30% strain showed significantly higher GAG/ dry weight than samples that were cultured in free-swell. On day 42, there was no significant difference between groups and there was a significant decrease in GAG when compared to day 28. Group loaded with 30% strain and then 10% (group 30/10) had significantly higher DNA/ dry weight than any group on day 42. However, there was no significant difference in DNA between groups in the initial 2 weeks of mechanical loading (day 28 of culture). Group 30/10 also had significantly higher collagen per dry weight than the free-swell group, however as seen with GAG, there was also a decrease in collagen for all groups when compared to day 28. There was no significant difference in collagen between groups after 2 weeks of mechanical loading (day 28 of culture). Equilibrium modulus (EM) increased for all groups between day 14 and day 28 of culture but decreased during day 42. On day 28, samples that received no loading and samples that received 10% strain had significantly higher EM than groups loaded with 30% strain. On day 42, group 10/10 had significantly higher EM than the free-swell group. Discussion: Dynamic loading significantly enhanced GAG production by chondrocytes in the first 28 days of culture. The samples were able to reach EM values consistent with native cartilage (60-250kPa) in the first 28 days. After 28 days, there was a decrease in GAG, collagen, and equilibrium modulus in all the groups. However, DNA production increased after 28 days indicating cell death did not contribute to the decrease in GAG, collagen, and mechanical properties. The results could indicate a decrease in chondrogenic characteristics. Although the data failed to prove the hypothesis, it did suggest that subjecting the samples with a strain of 30% and then 10% could lead to higher DNA and collagen deposition than freeswell. Advisors/Committee Members: Bilgen, Bahar (Advisor), Scott, Jeffrey (Reader), Borton, David (Reader).

Subjects/Keywords: tissue engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Vasquez, S. (2017). High and Low Compressive Loading on Tissue Engineered Cartilage. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733549/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Vasquez, Stephany. “High and Low Compressive Loading on Tissue Engineered Cartilage.” 2017. Thesis, Brown University. Accessed March 28, 2020. https://repository.library.brown.edu/studio/item/bdr:733549/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Vasquez, Stephany. “High and Low Compressive Loading on Tissue Engineered Cartilage.” 2017. Web. 28 Mar 2020.

Vancouver:

Vasquez S. High and Low Compressive Loading on Tissue Engineered Cartilage. [Internet] [Thesis]. Brown University; 2017. [cited 2020 Mar 28]. Available from: https://repository.library.brown.edu/studio/item/bdr:733549/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vasquez S. High and Low Compressive Loading on Tissue Engineered Cartilage. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733549/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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