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You searched for +publisher:"Brown University" +contributor:("Pins, George"). One record found.

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1. Susienka, Michael James. Quantifying the fusion and self-assembly of 3D microtissue building parts.

Degree: Biomedical Engineering, 2017, Brown University

Microtissue self-assembly and fusion are biological processes that are important to the fields of tissue engineering and biofabrication and particularly relevant to techniques such as three-dimensional (3D) bio-printing and microtissue stacking in which pre-formed building parts are brought together and then fused into a larger structure. The aim of this thesis was to study these complex phenomena in a more quantitative and higher-throughput fashion. First, we provided a comprehensive review of the current literature on spheroid fusion in the contexts of cancer biology and tissue engineering. Using non-adhesive agarose micro-molds, we formed scaffold-free spheroid microtissues composed of primary human chondrocytes or MCF-7, a human breast cancer cell line. We developed a straightforward method for rapidly assembling numerous spheroid doublets for wide-field fluorescence time-lapse imaging experiments. Using this technique, we found that the initial connection between two spheroids occurred rapidly and robustly. Additionally, we created a custom image analysis pipeline that allowed for measurement of the complex morphological changes that occur during fusion in a comprehensive and quantitative fashion. We identified a novel phenomenon of doublet rotation, which was found to be dependent upon cytoskeletal machinery. Following these spheroid fusion studies, we focused on the self-assembly and fusion of complex microtissues. By measuring the contraction of rod-shaped microtissues over time, we found that this contraction was dependent upon cellular seeding density and was inhibited at lower temperatures. We also studied microtissue intactness and found that toroid microtissues formed rapidly and that intactness was also inhibited at lower temperatures. Finally, we studied the fusion of misaligned stacks of toroid microtissues and found that small gaps between them were capable of self-closing during fusion. This study was motivated by the importance of lumen alignment during stacking and the need for design parameters about how much misalignment can be tolerated when stacking living building parts. Overall, this thesis advances our understanding microtissue self-assembly and fusion, both of which are critical for the manufacturing of larger tissues, organs, or other constructs that use individual microtissues as building parts. Advisors/Committee Members: Morgan, Jeffrey (Advisor), Darling, Eric (Reader), Pins, George (Reader), Schell, Jacquelyn (Reader), Tripathi, Anubhav (Reader).

Subjects/Keywords: tissue engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Susienka, M. J. (2017). Quantifying the fusion and self-assembly of 3D microtissue building parts. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733528/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Susienka, Michael James. “Quantifying the fusion and self-assembly of 3D microtissue building parts.” 2017. Thesis, Brown University. Accessed March 24, 2019. https://repository.library.brown.edu/studio/item/bdr:733528/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Susienka, Michael James. “Quantifying the fusion and self-assembly of 3D microtissue building parts.” 2017. Web. 24 Mar 2019.

Vancouver:

Susienka MJ. Quantifying the fusion and self-assembly of 3D microtissue building parts. [Internet] [Thesis]. Brown University; 2017. [cited 2019 Mar 24]. Available from: https://repository.library.brown.edu/studio/item/bdr:733528/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Susienka MJ. Quantifying the fusion and self-assembly of 3D microtissue building parts. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733528/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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