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You searched for +publisher:"Brown University" +contributor:("Lee, Jonghwan"). Showing records 1 – 2 of 2 total matches.

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1. Crowley, Shannon. Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy.

Degree: Biomedical Engineering, 2018, Brown University

Epilepsy affects over 3 million people in the United States alone. In many of those cases, the disease is refractory to medication, leaving patients limited in their daily lives. When oral medication does not mitigate or relieve seizure activity, neurosurgery is performed in order to remove the seizure centers of the brain. Herein lies the unmet need in the treatment of this disease. This research is the first step toward creating a novel intraoperative microscope that can delineate the boundary between healthy and epileptogenic tissue in order to assist surgeons in the surgical resection of the diseased tissue in real time. This research aims to use Optical Coherence Tomography (OCT) to quantify the changes before and after seizure activity in cortical tissue. In a rodent model, seizures were induced using pilocarpine and verified by electrocorticography (ECoG). OCT was utilized to quantify the scattering coefficient, blood flow, and the structure of cortical tissue. The optical scattering coefficient of the cortical tissue exhibited a significant increase after the induced seizure activity. This distinct change in the optical property of tissue, which can be detected by OCT in real time and without fluorescence, supports the technical feasibility of the proposed intraoperative microscope. Advisors/Committee Members: Lee, Jonghwan (Advisor), Asaad, Wael (Reader), Borton, David (Reader).

Subjects/Keywords: Optical coherence tomography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Crowley, S. (2018). Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792792/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Crowley, Shannon. “Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy.” 2018. Thesis, Brown University. Accessed October 16, 2019. https://repository.library.brown.edu/studio/item/bdr:792792/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Crowley, Shannon. “Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy.” 2018. Web. 16 Oct 2019.

Vancouver:

Crowley S. Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy. [Internet] [Thesis]. Brown University; 2018. [cited 2019 Oct 16]. Available from: https://repository.library.brown.edu/studio/item/bdr:792792/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Crowley S. Optical coherence tomography of cortical tissue in pilocarpine treated rats as a model for temporal lobe epilepsy. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792792/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Lee, Julia Seungmi. Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography.

Degree: School of Engineering, 2018, Brown University

Cellular viability represents whether a cell is performing normal functions, relating to intracellular energy synthesis. Accurately quantifying the cellular viability would facilitate novel studies on how pathological environments affect the functioning of cells in various diseases. Nevertheless, technologies for monitoring the cellular viability in live tissue models are currently lacking. This study aims at testing a recently developed technology, which integrates dynamic light scattering and optical coherence tomography (called DLS-OCT), to image the cellular viability with single-cell resolution. DLS analyzes fluctuations in light scattered by particles to measure diffusion or flow of the particles, and OCT uses coherence gating to collect light only scattered from a small volume for high-resolution structural imaging. Integrating the two technologies, DLS-OCT constructs high-resolution diffusion coefficient and flow velocity 3D maps. It is known that the motion of intracellular organelles, often called intracellular motility, resembles a random walk in the confined cytoplasm space, thus it can be quantified by the diffusion coefficient. Since the intracellular motility is correlated with the cell’s metabolism level, the diffusion coefficient map of DLS-OCT is expected to enable us to image the cellular viability. Here, the DLS-OCT imaging of cellular viability was validated by characterizing responses of the measured intracellular motility to the environmental conditions such as the temperature and pH, in animal retinal explant samples. First, we characterized our new OCT system and optimized scanning sequences and processing procedures for DLS-OCT data, to match the dynamic range of our DLS-OCT measurement with the typical range of intracellular motility. Both numerical simulation and phantom experiments were performed for optimization. Second, methods required for animal retinal explant experiments were established, and DLS-OCT data from retinal tissue while manipulating the cellular viability were acquired and analyzed, to test the technical hypothesis that DLS-OCT-measured intracellular motility of neurons significantly diminishes when the cellular viability levels are out of the physiological ranges. Similar operations were performed to tissue spheroids with additional morphological measurements. As a result, we measured individual cells’ healthiness for tempered conditions, which will enlighten studying cells’ healthiness during disease progress or therapeutic treatment in stroke, epilepsy, and Alzheimer’s disease among others. Advisors/Committee Members: Lee, Jonghwan (Advisor), Powers, Thomas (Reader), Hoffman-Kim, Diane (Reader).

Subjects/Keywords: Dynamic Light Scattering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lee, J. S. (2018). Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792773/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Lee, Julia Seungmi. “Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography.” 2018. Thesis, Brown University. Accessed October 16, 2019. https://repository.library.brown.edu/studio/item/bdr:792773/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Lee, Julia Seungmi. “Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography.” 2018. Web. 16 Oct 2019.

Vancouver:

Lee JS. Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography. [Internet] [Thesis]. Brown University; 2018. [cited 2019 Oct 16]. Available from: https://repository.library.brown.edu/studio/item/bdr:792773/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee JS. Cellular Viability Imaging Using Dynamic Light Scattering Optical Coherence Tomography. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792773/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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