Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"Brown University" +contributor:("Kuntz Willits, Rebecca"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Lopez-Fagundo, Cristina Y. Understanding and directing cellular behavior using bioinspired and biomimetic materials.

Degree: PhD, Biomedical Engineering, 2013, Brown University

The research presented in this thesis aims to advance our understanding of cellular behavior in physiologically relevant environments composed of single cues or multiple cues. The cues explored include topographical cues, mechanical cues, biochemical cues and the combination thereof. First, we were motivated by Schwann cells (SCs), the support cells of the peripheral nervous system (PNS), and ask how after their migration to an injury site their topographical features aid neuronal guidance. By generating substrates inspired by the features and dimensions of SC, we find that SC processes are optimal for guidance and SC somas are sufficient for guidance. We also find that neurite response to these bioinspired materials was time-dependent. Next, we wanted to investigate the functional and behavioral effects of substrate stiffness on SCs. For these studies we cultured SCs on polyacrylamide (PA) substrates with varying stiffnesses and find that substrate stiffness can regulate SC phenotype as well as the magnitude of the interactions with the underlying material, possibly by cytoskeletal regulation, distribution and re-arrangement. To examine the added effect of biochemical guidance cues on substrate stiffness, we cultured SCs on PA substrates micropatterned with laminin (LN). In the presence of LN stripes, SCs exhibit a migrating phenotype different than the one observed on flat materials uniformly coated with LN. These results are important when considered in light of novel design strategies aimed at promoting SC migration and infiltration to aid repair after an injury in the PNS. We further examine the effect of environmental cues by the design and fabrication of a platform that can be tailored to the cues presented by a number of different environments, including the one required for the proliferation and maintenance of self-renewal of mouse embryonic stem cells (mESCs). The fabrication of an acellular biomimetic feeder layer with the mechanical and topographical properties of mouse embryonic fibroblasts that promotes colony formation and maintains stemness further confirmed the significance of employing biomimetic and bioinspired approaches to better understand cellular behavior in multiple scenarios. Advisors/Committee Members: Hoffman-Kim, Diane (Director), Morgan, Jeffrey (Reader), Darling, Eric (Reader), Franck, Christian (Reader), Kuntz Willits, Rebecca (Reader).

Subjects/Keywords: Schwann cells

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lopez-Fagundo, C. Y. (2013). Understanding and directing cellular behavior using bioinspired and biomimetic materials. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:386148/

Chicago Manual of Style (16th Edition):

Lopez-Fagundo, Cristina Y. “Understanding and directing cellular behavior using bioinspired and biomimetic materials.” 2013. Doctoral Dissertation, Brown University. Accessed March 20, 2019. https://repository.library.brown.edu/studio/item/bdr:386148/.

MLA Handbook (7th Edition):

Lopez-Fagundo, Cristina Y. “Understanding and directing cellular behavior using bioinspired and biomimetic materials.” 2013. Web. 20 Mar 2019.

Vancouver:

Lopez-Fagundo CY. Understanding and directing cellular behavior using bioinspired and biomimetic materials. [Internet] [Doctoral dissertation]. Brown University; 2013. [cited 2019 Mar 20]. Available from: https://repository.library.brown.edu/studio/item/bdr:386148/.

Council of Science Editors:

Lopez-Fagundo CY. Understanding and directing cellular behavior using bioinspired and biomimetic materials. [Doctoral Dissertation]. Brown University; 2013. Available from: https://repository.library.brown.edu/studio/item/bdr:386148/

.