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You searched for +publisher:"Brown University" +contributor:("Darling, Eric"). Showing records 1 – 2 of 2 total matches.

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1. Shah, Manisha. Role of Mechanophenotype in Cell-Substrate Interactions.

Degree: Department of Molecular Pharmacology, Physiology and Biotechnology, 2018, Brown University

Cellular mechanophenotype is defined as the elastic and viscoelastic properties of a cell, and is commonly characterized by atomic force microscopy (AFM). Assessment of a cell’s mechanophenotype in the larger context of its microenvironment can add to increased understanding of biological phenomena such a cellular organization, proliferation, shape, motility, and differentiation. Here, we aim to (1) characterize the mechanical properties and differentiation potential of cells present in human lipoaspirate, (2) examine the influence of inherent mechanophenotype on cell-cell-substrate organization, and (3) assess incorporation of compliant microparticles into cancer vs. normal spheroids. Advisors/Committee Members: Darling, Eric (Advisor), Morgan, Jeffrey (Reader), Wong, Ian (Reader), Coulombe, Kareen (Reader), Slater, John (Reader).

Subjects/Keywords: Atomic force microscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Shah, M. (2018). Role of Mechanophenotype in Cell-Substrate Interactions. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792653/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Shah, Manisha. “Role of Mechanophenotype in Cell-Substrate Interactions.” 2018. Thesis, Brown University. Accessed March 25, 2019. https://repository.library.brown.edu/studio/item/bdr:792653/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Shah, Manisha. “Role of Mechanophenotype in Cell-Substrate Interactions.” 2018. Web. 25 Mar 2019.

Vancouver:

Shah M. Role of Mechanophenotype in Cell-Substrate Interactions. [Internet] [Thesis]. Brown University; 2018. [cited 2019 Mar 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:792653/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shah M. Role of Mechanophenotype in Cell-Substrate Interactions. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792653/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. González Cruz, Rafael D. Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells.

Degree: Biomedical Engineering, 2018, Brown University

Adipose-derived stem cells (ASCs) represent a promising cell source for cell-based therapies and tissue engineering applications because of their multipotency and abundance. However, these cells are mechanically heterogeneous and exist in subpopulations that exhibit variability in their differentiation potential. Therefore, their widespread use in clinical applications is limited by the number of actual stem cells that can differentiate into desired lineages for a particular lineage. In this thesis, I present evidence to confirm the validity of the following scientific hypotheses: (a) cellular mechanical properties can serve as physical biomarkers to identify lineage-specific differentiation potential in clonally-derived ASCs (Chapter 2); (b) cellular mechanical properties to change across passage numbers and are important parameters to consider in ASC expansion protocols and differentiation efficiency (Chapter 3); and (c) lamin protein C serves as a potential macromolecular biomarker for whole-cell mechanophenotype and is dependent on intact cytoskeletal structures in stiff but not compliant cells (Chapter 4). I also present preliminary data that suggests that lamin A/C proteins can be used to characterize and sort cells with drastically different mechanophenotypes (Appendixes A and B). Altogether, the collection of studies hereby presented proposes the use of lamin A/C proteins for high-throughput sorting and characterization of cells with distinct mechanophenotype, such as stem cells, in heterogenous samples. Advisors/Committee Members: Darling, Eric M. (Advisor), Hoffman-Kim, Diane (Reader), Shukla, Anita (Reader), Franck, Christian (Reader), Dahl, Kris Noel (Reader).

Subjects/Keywords: Stem cells – Research

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

González Cruz, R. D. (2018). Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792667/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

González Cruz, Rafael D. “Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells.” 2018. Thesis, Brown University. Accessed March 25, 2019. https://repository.library.brown.edu/studio/item/bdr:792667/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

González Cruz, Rafael D. “Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells.” 2018. Web. 25 Mar 2019.

Vancouver:

González Cruz RD. Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells. [Internet] [Thesis]. Brown University; 2018. [cited 2019 Mar 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:792667/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

González Cruz RD. Analysis of Cellular Mechanical Properties and Nuclear Lamin Proteins As Biomarkers for Whole-Cell Mechanophenotyping of Adipose-Derived Stem Cells. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792667/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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