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You searched for +publisher:"Brown University" +contributor:("Chickering, Donald"). One record found.

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1. Labriola, Nicholas Ryan. The Role of Mechanical Properties in Stem Cell Adipogenesis.

Degree: Biomedical Engineering, 2016, Brown University

Abstract of “The Role of Mechanical Properties in Stem Cell Adipogenesis” by Nicholas R. Labriola, Ph.D., Brown University, May 2017 The mechanical properties of cells and their substrates have important biological implications, especially for stem cell differentiation. Individual cells within stem cell populations exhibit varying differentiation potentials for specific lineages and respond uniquely to chemical or mechanical cues. The first study described herein involved characterizing the heterogeneity in differentiation response and mechanical properties of single cells within adipogenically differentiating populations of adipose-derived stem cells (ASCs) in two-dimensional monolayers. The upregulation of the adipogenic gene, peroxisome proliferator-activated receptor gamma (PPARG), was associated with a drastic drop in the elastic modulus of individual cells. The percent of the population expressing PPARG increased gradually in samples provided adipogenic induction medium, while control samples maintained a constant level of expression. This study showed that the gradual decrease in elasticity of adipogenically differentiating populations, previously reported in literature, is driven by a sub-population of cells responding to the induction cues. This stresses the need to consider heterogeneity in stem cell differentiation responses when drawing conclusions, as population-based assessments can mask underlying contributions from the sub-populations that drive such changes. The second study involved the generation of mechanically distinct and stable polyacrylamide microbeads using inverse emulsification to mimic the size and elasticity of living cells. The third study involved delivering these microbeads to ASCs in three-dimensional spheroid cultures to determine the effects of mechanical cues on adipogenic differentiation response and whole-spheroid mechanophenotype, both with and without chemical induction factors. Results showed little change in the expression of lineage-specific mRNA in response to substrate stiffness for either three-dimensional, or paired two-dimensional cultures, both with and without induction factors. Three-dimensional spheroids of ASCs stiffened in accordance with the elasticity of the incorporated microbeads when cultured with adipogenic medium, and became more compliant and dissociated when cultured with microbeads stiffer than the cells themselves without chemical induction factors present. This demonstrates that ASCs still respond to mechanical properties in three-dimensional culture, however, there was little change in the differentiation response as measured by lineage-specific gene expression. Additional research will focus on distributing the microbeads more evenly throughout spheroids to increase the number of cells exposed to these mechanical cues. Advisors/Committee Members: Darling, Eric (Advisor), Chickering, Donald (Reader), Morgan, Jeffrey (Reader), Franck, Christian (Reader), Tripathi, Anubhav (Reader).

Subjects/Keywords: Stem cells – Research

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Labriola, N. R. (2016). The Role of Mechanical Properties in Stem Cell Adipogenesis. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733393/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Labriola, Nicholas Ryan. “The Role of Mechanical Properties in Stem Cell Adipogenesis.” 2016. Thesis, Brown University. Accessed March 25, 2019. https://repository.library.brown.edu/studio/item/bdr:733393/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Labriola, Nicholas Ryan. “The Role of Mechanical Properties in Stem Cell Adipogenesis.” 2016. Web. 25 Mar 2019.

Vancouver:

Labriola NR. The Role of Mechanical Properties in Stem Cell Adipogenesis. [Internet] [Thesis]. Brown University; 2016. [cited 2019 Mar 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:733393/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Labriola NR. The Role of Mechanical Properties in Stem Cell Adipogenesis. [Thesis]. Brown University; 2016. Available from: https://repository.library.brown.edu/studio/item/bdr:733393/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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