+publisher:"Amrita Vishwa Vidyapeetham (University)" +contributor:("Nair, Shantikumar V")

1. Sathy, Binulal Nelson. Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -.

Degree: Engineering, 2013, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/13052

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Tissue engineering is an interdisciplinary approach which combines cell biology newlineand materials science with the purpose of developing implants using live cells and newlinesynthetic extracellular… (more)

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Tissue engineering is an interdisciplinary approach which combines cell biology newlineand materials science with the purpose of developing implants using live cells and newlinesynthetic extracellular matrix. This thesis attempts this approach in order to newlinedevelop an alternative for autologous bone grafts. Electrospinning is one of the newlinewidely used techniques to fabricate polymeric fibers mimicking the structural newlinedimensions of extracellular matrix for bone tissue engineering applications. newlineHowever current bone tissue engineering approaches using electrospun nanofibers newlinedemand a construct design to address several major challenges in the field. newlineTherefore this thesis work is focussed on developing a construct design which can newlineaddress challenges such as cell infiltration and vascularisation in the construct, newlinecontrolling the degradability of the electrospun fibers, and the scalability of the newlineconstruct. In the first part of this PhD work we have extensively evaluated newlineelectrospun Poly (and#1028;-caprolactone) (PCL) nano-fibers, micro-fibers and human newlinemesenchymal stem cells (hMSCs) for bone tissue engineering purpose. Our results newlineindicated that hMSCs attached and spread rapidly on nano-fibrous scaffolds in newlinecomparison to micro-fibrous PCL. Further, hMSCs proliferated well on the nanofibrous newlinescaffolds. The cells on the nano-fibrous PCL were found to differentiate newlineinto the osteoblast lineage and subsequently mineralize upon addition of in vivo newlineosteogenic regulators. The attachment and spreading of hMSCs were more newlineeffective on the nano-fibers compared with the micro-fibers despite the lower newlineprotein surface coverage (total adsorbed protein per unit fiber surface area) on newlinenano-fibers. Thereafter, nano-fibrous semi-synthetic polymeric nanocomposite newlinescaffolds were engineered by incorporating a maximum of 15 wt. % biopolymeric newlinegelatin nanoparticles (nG) into the synthetic polymer PCL prior to electrospinning newlinein order to impart degradability to the nano-fibers.

References given chapter wise

Advisors/Committee Members: Nair, Shantikumar V.

Subjects/Keywords: Nano science; Electrospun nano-fibrous matrix; bone tissue engineering,; marrow derived mesenchymal; electrospun PCL Nano; Micro fibers

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sathy, B. N. (2013). Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/13052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sathy, Binulal Nelson. “Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -.” 2013. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/13052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sathy, Binulal Nelson. “Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -.” 2013. Web. 12 Dec 2019.

Vancouver:

Sathy BN. Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13052.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sathy BN. Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering; -. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13052

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Ganesh, Nitya. Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -.

Degree: Engineering, 2013, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/13056

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In this study, we report our work on the incorporation of ceramic nanofillers, within a poly(caprolactone) (PCL) nanofibrous scaffold by electrospinning and their advantages over… (more)

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In this study, we report our work on the incorporation of ceramic nanofillers, within a poly(caprolactone) (PCL) nanofibrous scaffold by electrospinning and their advantages over the native PCL scaffold for bone tissue engineering applications. PCL is biocompatible, biodegradable, FDA approved biomaterial. It is one of the strong candidates for bone tissue engineering. It is bioinert and has good mechanical strength as compared to polymers like PLGA and chitosan. This thesis focuses on the incorporation of different ceramic nanofillers in nanofibrous PCL scaffolds and the changes caused due to its incorporation in the properties of the scaffold, in terms of their hydrophobicity, biocompatibility, mechanical strength and osteogenic potential. Ceramics are hard and brittle materials, mostly known to be osteoconductive. Ceramics while in a composite give the mechanical strength to polymers. They can also increase the biological response especially the bioactivity of the scaffold. Nanofillers used in our study were namely, silica nanopowder (nSiO2), halloysite nanoclay (NC), nano hydroxyapatite (nHAp) and multifunctional nano hydroxyapatite (MF-nHAp). Such a study involving these four particular PCL/nanofiller composite scaffolds for assessing their regenerative potential for the purpose of bone tissue engineering may not have been done ever before in our opinion. Here, in our study, we wanted to take advantage of properties of nanoceramics (nSiO2, NC, nHAp and MF-nHAp) for reducing the hydrophobicity of PCL and enhancing the scaffold properties like strength and osteogenic potential for bone tissue engineering. Also, we wanted to exploit the magnetic resonance (MR) contrast enhancement ability of the MF-nHAp. These MF-nHAp incorporated scaffolds were engineered in order to enhance the osteogenic potential as well as its MR functionality for its application in bone tissue engineering.

References p.71-86, Annexture p.87-89

Advisors/Committee Members: Nair, Shantikumar v.

Subjects/Keywords: Tissue engineering; scaffolds; Ceramic Nanofillers; Nanoceramics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ganesh, N. (2013). Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/13056

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ganesh, Nitya. “Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -.” 2013. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/13056.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ganesh, Nitya. “Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -.” 2013. Web. 12 Dec 2019.

Vancouver:

Ganesh N. Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13056.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ganesh N. Enhancing the osteogenic potential of the polymeric nanofibrous scaffolds by the incorporation of ceramic nanofillers for Bone Tissue Engineering Applications; -. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13056

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Abhilash Sasidharan. Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -.

Degree: Engineering, 2012, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/6038

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Nanomaterials possess unique physicochemical properties such as high reactivity, large surface to volume ratio, exceptional electronic characteristics and quantum size effects. A probable downside of… (more)

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Nanomaterials possess unique physicochemical properties such as high reactivity, large surface to volume ratio, exceptional electronic characteristics and quantum size effects. A probable downside of these remarkable properties would be their enhanced interactions with biological systems, which may lead to potential toxic effects. Zinc oxide (ZnO) and graphene are two important nanomaterials that are extensively studied by the nanoscience community owing to their unique physico-chemical properties rendering wide range of potential applications. In the current Ph.D. work, we have extensively studied the specific role of size scale, and surface chemistry of zinc oxide nanocrystals (ZnO NCs) on its toxicity towards prokaryotic and eukaryotic cells. The results show that the toxicity towards bacteria increased with reduction in particle size (40nm and#8722;1.2µM) and with increasing concentrations (1mM and#8722; 7.5mM). Flow cytometry and confocal microscopy studies revealed that ZnO NCs undergo rapid dissolution in acidic (pH ~ 5-6) cancer microenvironment causing elevated ROS stress, mitochondrial superoxide formation, depolarization of mitochondrial membrane, and cell cycle arrest at S/G2 phase leading to apoptosis whereas normal cells having neutral pH remained unaffected at the same concentration. These results point to the interesting role of dissolution chemistry of nanomaterials under various bio-chemical environments in determining their toxicity. In case of graphene, where dissolution is a remote possibility, we have studied the role of surface chemistry in its nano-bio interaction. The interaction of graphene with kidney epithelial cells and macrophage cells showed that pristine graphene (p- G) accumulated preferentially on the plasma membrane leading to high oxidative stress. In contrast, carboxyl functionalized hydrophilic graphene (f-G) was internalized by the cells without causing much toxicity up to 48 h.

References given chapters wise

Advisors/Committee Members: Manzoor, Koyakutty, Nair, Shantikumar V.

Subjects/Keywords: Nanosciences; Molecular Medicine; nanosystems; ZnO Nanocrystals; Cell culture; Graphene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sasidharan, A. (2012). Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/6038

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sasidharan, Abhilash. “Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -.” 2012. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/6038.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sasidharan, Abhilash. “Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -.” 2012. Web. 12 Dec 2019.

Vancouver:

Sasidharan A. Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/6038.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sasidharan A. Nano-bio interactions of Zno and graphene nanosystems: understanding the potential in vitro and in vivo response; -. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/6038

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Divya Rani, V V. In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -.

Degree: Nanosciences and Molecular Medicine, 2012, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/4924

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An important aspect of orthopedic implant integration is the enhancement of functional activity of osteoblasts at the tissue-implant interface without any fibrous tissue intervention. Nanostructured… (more)

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An important aspect of orthopedic implant integration is the enhancement of functional activity of osteoblasts at the tissue-implant interface without any fibrous tissue intervention. Nanostructured implant surfaces are known to enhance osteoblast activity. Objective of this thesis work is to develop simple and cost effective method for the fabrication of nanostructures on titanium implants showing good biocompatibility and compare the influence of different nanostructures on osteoblast response in vitro as well as in vivo. A set of nonperiodic structures ranging from mesoporous, nanoscaffolds, nanoflowers, nanoneedles, nanorods and octahedral bipyramids were fabricated by systematically tuning the hydrothermal conditions such as reaction medium composition, concentration, temperature and time duration. All nanomodified titanium surfaces were characterized by Scanning Electron Microscopy, Energy Dispersive Analysis, X-ray Diffraction, Transmission Electron Microscopy, High Resolution TEM and Electron Diffraction. Surface area analysis by dye adsorption test showed that all the nanosurface modified samples have an increased surface area in comparison to polished Ti surface. Adhesion strength analysis using microscratch test revealed good adhesion strength of nanoscaffold and nanoneedular structures. Further a detailed in vitro cellular response as well as in vivo osteointegration studies was carried out on selected nanostructures such as nanoscaffold (NS), nanoleaf (NL), Nanoneedles (NN) in comparison to electrochemically modified nanotube (NT) as well as control polished Ti (NP). Our studies showed that, a specific surface nanomorphology, viz., nanoleaves, which is a network of vertically aligned, non-periodic, leaf-like structures with thickness in the nanoscale, provided a distinct increase in osteoblast cell proliferation, alkaline phosphatase (ALP) activity and collagen synthesis, compared to several other types of nanomorphologies such as nanotubes, nanoscaffold and nanoneedles.

References given chapters wise

Advisors/Committee Members: Nair, Shantikumar V, Menon, Deepthy.

Subjects/Keywords: Implant failure; Physical vapor Deposition; Cell Culture; Nanosciences; Molecular Medicine

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Divya Rani, V. V. (2012). In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/4924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Divya Rani, V V. “In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -.” 2012. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/4924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Divya Rani, V V. “In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -.” 2012. Web. 12 Dec 2019.

Vancouver:

Divya Rani VV. In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Divya Rani VV. In vitro cellular response and in vivo osteointegration studies on nanostructured metallic titanium implants; -. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Mini P A. Integration of nanomaterials for photovoltaic energy generation and storage.

Degree: Nanosciences, 2012, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/8878

None

References included in chapters, List of Publications p. 182-183

Advisors/Committee Members: Nair, Shantikumar V, Subramanian, K R V.

Subjects/Keywords: Nanosciences; Quantum dot sensitized solar cell; Electrochemical capacitors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

A, M. P. (2012). Integration of nanomaterials for photovoltaic energy generation and storage. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/8878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

A, Mini P. “Integration of nanomaterials for photovoltaic energy generation and storage.” 2012. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 12, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/8878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

A, Mini P. “Integration of nanomaterials for photovoltaic energy generation and storage.” 2012. Web. 12 Dec 2019.

Vancouver:

A MP. Integration of nanomaterials for photovoltaic energy generation and storage. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. [cited 2019 Dec 12]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/8878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

A MP. Integration of nanomaterials for photovoltaic energy generation and storage. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/8878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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