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Title Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal
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Date Available
Degree Level masters
University/Publisher Universidade Federal de Alagoas
Abstract

Candida species have emerged as the second most common cause of vulvovaginitis in the world, producing infections that can cause unwanted symptoms such as itching, discharge, dyspareunia and burning. The objective of this study was to evaluate the genetic variability and the antifungal susceptibility profile of Candida yeasts isolated from vaginal secretions of patients Maceió - Alagoas. The yeasts were collected weekly in the sector of five clinical microbiology laboratories located in the city of Maceió between March and December 2010. For each isolate was performed through a purification of seeding a suspension of yeast in YPD solid medium. DNA extraction was performed by phenol / chloroform and identification by PCR with primers species specific for C.albicans, C. glabrata, C. tropicalis, C. krusei and C. parapsilosis. The patients with vaginal candidiasis were evaluated for the presence of symptoms and risk factors for acquiring the disease. The patterns of sensitivity to ketoconazole, fluconazole, itraconazole and amphotericin B were evaluated according to CLSI standards (M27-A2).Molecular typing was used in the microsatellite (GTG) 5 and the fragments separated on 1.5% agarose gel at 75 volts / cm for 120 minutes, stained with ethidium bromide and viewed on photodocumentation system. Banding patterns were analyzed by BioNumerics 6.5 soltware to obtain electropherogram using the similarity coefficient Jacard. We obtained 296 isolates of Candida yeasts, and C. albicans (82.45%) the most frequent species, followed by C. glabrata with 7.75%, C. parapsilosis with 4.05%, C. tropicalis witn 3.75% and C. krusei 2%. These isolates exhibited resistance profile of 66,7% for amphotericin B, fluconazole for 52,6%, 89,7% for itraconazole and to ketoconazole 21,3%. Among the risk factors for acquiring the disease stands out pregnancy with 24.66% and history of VVC in 15.52%. The main clinical sign was presented discharge in 77,13% of cases. The species analyzed showed a high diversity for the genetic marker (GTG)5, with values ranging from 0.7470 to 0.9963, which allowed the formation of genetic patterns 172 for C. albicans, 10 for C. tropicalis, 11 for C. parapsilosis, 8 for C. glabrata and 4 for C. krusei. The incidence of Candida species with a profile of resistance to itracozaol, amphotericin B, fluconazole and ketoconazole, provides epidemiological data for the study area and reinforces the importance of identifying yeasts to the species level and testing of in vitro antifungal susceptibility.

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As espécies de Candida têm emergido como a segunda causa mais comum de vulvovaginites no mundo, produzindo infecções, que podem causar sintomas indesejáveis como, prurido, corrimento, dispareunia e ardor. O objetivo desse estudo foi avaliar a variabilidade genética e o perfil de susceptibilidade aos antifúngicos em leveduras do gênero Candida isoladas de secreção vaginal de pacientes de Maceió Alagoas. As amostras foram coletadas no setor de microbiologia clínica…

Subjects/Keywords Candida; Susceptibilidade aos antifúngicos; PCR; Caracterização genética; (GTG)5; Candida; Antifungal susceptibility; PCR; Genetic characterization; (GTG)5; CNPQ::CIENCIAS DA SAUDE
Contributors Silva Filho, Eurípedes Alves da; CPF:16649630497; SILVA FILHO, E. A.; Azevedo, Dalmo Almeida de; CPF:49935275949; http://lattes.cnpq.br/4202083703695616; Neves, Rejane Pereira; CPF:21401365434; http://lattes.cnpq.br/0360951033804105; Araújo, Maria Anilda dos Santos; CPF:75900220471; ARAÚJO, M. A. S.
Language pt
Rights Acesso Embargado
Country of Publication br
Format application/pdf
Record ID oai:www.repositorio.ufal.br:riufal/955
Other Identifiers LIMA, Gabryelle Barbosa Cordeiro de. Genetic variability and profile of susceptibility to antifungal Candida species in isolated from vaginal secretion. 2012. 102 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Alagoas, Maceió, 2012.
Repository brazil-ufal
Date Retrieved
Date Indexed 2016-02-10

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