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Title Epidemiologia molecular de beta-lactamases de espectro estendido (ESBL) e carbapenemase KPC produzidas por enterobactérias isoladas de pacientes de Alagoas
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Publication Date
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Degree Level masters
University/Publisher Universidade Federal de Alagoas
Abstract

Bacterial resistance is one of the worldwide public health issues. This work aimed to genetically characterize species of the family Enterobacteriaceae phenotipic producing ESBL and KPC obtained from patients of Alagoas. Bacteria were identified by semi-automated tests. Confirmed as producing ESBL and KPC by phenotypic screening tests. The antimicrobial in vitro susceptibility test was performed by disk-diffusion method. DNA was extracted by boiling method at 95ºC. The resistance genes blaTEM, blaCTX-M, blaSHV e blaKPC were identified with specific primers and genetic typing was performed by PCR with the microsatellite (GTG)5. 254 isolates of enterobacteria were obtained, of which 92,12% (234/254) had some of the genes blaTEM, blaCTX-M, blaSHV or blaKPC, 87,18% (204/234) ESBL (blaTEM, blaCTX-M, blaSHV) and 12,82% (30/234) KPC (blaKPC). Of these 234 isolates, 4,7% (11/234) were community-acquired infections with genes that express ESBL and 95,3% (223/234) of hospital infections, of which 86,55% (193/223) ESBL and 13,45% (30/223) KPC. BlaCTX-M (> 80%) was the most frequent type gene in enterobacteria. Urinary infections were the most frequent cases of infection in the community by Escherichia coli (54,55%) and Klebsiella pneumoniae (39,46%) in the hospital. BlaKPC was identified only in bacteria of hospital infections, especially in K. pneumoniae (30%). At the ICUs (38,57%) were obtained the most number of isolates producing ESBL and KPC. These enterobacteria showed multidrug resistance phenotypes with high levels for aminoglycosides, fluoroquinolones and sulfamethoxazole/trimethoprim. Associations between genotypes and antibiotic resistance were observed. Cases of clonal spread were identified in the hospital of Alagoas by enterobacteria producing ESBL and KPC. There is a predominance of genes blaTEM, blaCTX-M, blaSHV and blaKPC among isolates of enterobacteria resistant to beta-lactam, with the prevalence of the genetic element blaCTX-M. The clonal spread have contributed to the high levels of beta-lactam resistance among isolates of enterobacteria at hospitals in this study.

Fundação de Amparo a Pesquisa do Estado de Alagoas

A resistência bacteriana representa um dos problemas mundiais de saúde pública. Este trabalho teve como objetivo caracterizar geneticamente espécies da família Enterobacteriaceae produtoras fenotípicas de ESBL e KPC obtidas de pacientes de Alagoas. As bactérias foram identificadas por testes semi-automatizados. Confirmadas como produtoras de ESBL e KPC por testes fenotípicos de triagem. O teste de susceptibilidade in vitro aos antimicrobianos foi realizado pelo método de disco-difusão. O DNA foi extraído pelo método de fervura à 95ºC. Os genes de resistência blaTEM, blaCTX-M, blaSHV e blaKPC foram identificados com oligonucleotídeos específicos e a tipagem genética foi realizada pela PCR com o microssatélite (GTG)5. Foram obtidos 254 isolados de enterobactérias, dos quais 92,12% (234/254) apresentaram alguns dos genes blaTEM, blaCTX-M, blaSHV ou blaKPC, sendo 87,18% (204/234) ESBL…

Subjects/Keywords ESBL; KPC; Infecção hospitalar; Infecção comunitária; Disseminação clonal; (GTG)5; ESBL; KPC; Hospital infection; Community infection; Clonal spread; (GTG)5; CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA::EPIDEMIOLOGIA
Contributors Silva Filho, Eurípedes Alves da; CPF:16649630497; SILVA FILHO, E. A.; Ferreira, Sonia Maria Soares; CPF:29912989449; http://lattes.cnpq.br/1584568707943074; Azevedo, Dalmo Almeida de; CPF:49935275949; http://lattes.cnpq.br/4202083703695616; Tovar, Francisco Javier; CPF:02177610702; http://lattes.cnpq.br/2366497420587582
Language pt
Rights Acesso Embargado
Country of Publication br
Format application/pdf
Record ID oai:www.repositorio.ufal.br:riufal/949
Other Identifiers PIRES, Luana Luzia Santos. Molecular epidemiology of beta-lactamases extended spectrum (ESBL) and KPC carbapenemase enterobacteriaceae isolated produced by patients of Alagoas. 2011. 108 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Alagoas, Maceió, 2011.
Repository brazil-ufal
Date Retrieved
Date Indexed 2016-02-10

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…simples repetidas SUT Sulfametoxazol/trimetoprim T TEM TBE Tampo tris-borato-EDTA TE Tris-HCl EDTA TZB Tazobactam UCIs Unidades de Cuidados Intensivos UFC Unidade formadora de colnia UFAL Universidade Federal de Alagoas UNCISAL…

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