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Title synthesis of sulfated polysaccharides for colitis mice model
URL
Publication Date
Degree MS
Discipline/Department Chemistry
Degree Level thesis
University/Publisher Georgia State University
Abstract ABSTRACT IBD (inflammatory bowel disease) is a pandemic chronic disease of the gastrointestinal tract inducing Ulcerative colitis (UC) and Crhon disease (CD). The inflammation of colon especially associated with the growing risk of developing colon cancer. To extend the research on IBD, chemical mice models are investigated on easy way to induce colitis in rodent. Dextran sulfate salt (DSS) 7 days, 3% in drinking water induce IBD on mice. One of the problem of DSS is that it requires a purification step of mRNA by LiCl. This step induce this mRNA harvest rate quantitatively and qualitatively. In this study, we synthesized Pullulan Sulfate and Cyclodextrin Sulfate as alternative colitis inducers in mice. The specificity of sulfated polysaccharides in colon versus small intesinte has been confirmed by common gastrointestinal method (pro inflammatory cytokines causes degradation of the epithelium,and the presence of blood in stool.) INDEX WORDS: IBD, mice model, DSS, mRNA purification, Pullulan and Cyclodextrin.
Subjects/Keywords IBD; mice model; DSS; mRNA purification; Pullulan and Cyclodextrin.
Contributors Dr. Hamed Laroui
Country of Publication us
Record ID oai:scholarworks.gsu.edu:chemistry_theses-1071
Repository georgia-state
Date Retrieved
Date Indexed 2018-01-11
Created Date 2015-04-28 07:00:00

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…8 Figure 5: dextran sulfate sodium. ......................................................................... 9 Figure 6: pullulan ................................................................................................ 10 Figure 7: beta…

…cyclodextrin. ................................................................................ 11 Figure 8: synthesis reaction ................................................................................ 12 Figure 9: comparison of Pullulan and PS 13C NMR…

…to move mice.. Error! Bookmark not defined.27 Figure 13: colon of pullulan group. ..................................................................... 27 Figure 14: colon of PS II group…

…28 Figure 15: small intestine of pullulan group. ....................................................... 28 Figure 16 small intestine of pullulan sulfate II group. .......................................... 29 Figure 17: colon length of pullulan, PS I…

…PS II, CS and DSS. Compare to PS II and DSS treated mice colon, those treated with Pullulan and PS I mice colon is longer. The shorter colon is a typical character of inflammation colon. ..................................... 29 x Figure 18: small…

…intestine length of pullulan, CS PS I, PS II and DSS. Compare to PS II and DSS treated mice colon, those treated with Pullulan and PS I mice colon is at same length. Which is suggesting a healthy small intestine. ......................................... 30…

…32 Figure 22: qPCR data of colon, IL-10 is chosen as primer. PS II and DSS have an incredible increment compare to Pullulan and PS I. thus inflammation is detected in PS II and DSS treated tissue…

…33 Figure 23: qPCR data of colon, Cox 2 is chosen as primer. PS II and DSS have an incredible increment compare to Pullulan and PS I. thus inflammation is detected in PS II and DSS treated tissue…

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