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Title Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts
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Date Accessioned
Degree PhD
Discipline/Department Food Science
Degree Level doctoral
University/Publisher North Carolina State University
Abstract The objectives of this research were i) to optimize extraction of phenolic compounds from muscadine grape seeds using food grade solvents, ii) to characterize and quantify the phenolic compounds present in Carlos muscadine grape seed extracts, iii) to assess the oxidative stability of the grape seed extract, iv) to evaluate the antimicrobial activity of the optimal muscadine grape seed extract, and v) to evaluate the effect of muscadine grape seed extract on MCF-10A breast cells. Carlos muscadine grape seeds were manually removed from the skin and pulp, then freeze-dried or oven-dried. Phenolic compounds were extracted from muscadine grape seeds using a Dionex Accelerated Solvent Extractor 200 following a two factor composite design. The two factors were solvent and temperature. Ethanol in combination with water and ethyl acetate in combination with water were evaluated in this research. Solvent percentages ranged from 35% to 95% and temperature ranged from 75C to 150C. Optimal extraction conditions were determined using the Folin-Ciocalteu and oxygen radical absorbance capacity assays. Identification and quantification of the phenolic compounds were determined by high performance liquid chromatography with mass spectroscopy. The optimal extraction conditions for the highest recovery of phenolic compounds consisted of the freeze-dried seeds extracted with 65% ethanol and 35% water at 150°C, with total phenolic and oxygen radical absorbance capacity values of 5.87 mg GAE⁄g and 60.32 M-TE⁄g of seeds, respectively. The four major phenolic compounds quantified in the muscadine grape seed extract were epicatechin, catechin, gallic acid and ellagic acid. Oxidative stability index of the muscadine grape seed extract resulted in an induction period of 4.6 hours. In the antimicrobial study, both muscadine grape seed extract and ellagic acid inhibited the growth of E. coli 0157:H7, Listeria monocytogenes, and Salmonella typhimurium at 104 cfu⁄ml. These research findings show that muscadine grape seed extracts contain phytochemicals with antioxidant and antimicrobial properties that can be used in applications including food ingredients, food supplements and other nutraceutical applications.
Subjects/Keywords antioxidants; anti-microbial; anti-carcinogenic; grape seed; polyphenols; muscadines
Contributors Dr. Leon Boyd, Committee Chair (advisor)
Rights I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.
Country of Publication us
Record ID oai:repository.lib.ncsu.edu:1840.16/5328
Repository ncsu
Date Retrieved
Date Indexed 2018-12-06
Issued Date 2007-12-14 00:00:00

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…7. Bioavailability of polyphenols ………………………………………… 23 8. Subclasses of flavonoids ……………………………………………… 24 9. Schematic diagram of the ORAC assay principle ……………………… 25 Chapter III 1. Flow diagram of muscadine grape seed preparation for extraction…

GRAPE SEED EXTRACTS ……………………………………………… 70 Abstract ………………………………………………………………… 70 Introduction …………………………………………………………… 72 Materials and Methods ………………………………………………… 74 Results and Discussion ………………………………………………… 77 Conclusions…

…78 References ……………………………………………………………… 89 V. EFFECTS OF MUSCADINE GRAPE SEED EXTRACTS ON THE IN VITRO ACTIVITY OF MCF-10A IMMORTALIZED BREAST CELLS ……………………………………………………………………… 91 Abstract ………………………………………………………………… 91 Introduction…

…Muscadine Grapes (Carlos) and Grape Parts (mg/100g)………………………………………………………. 19 Chapter III 1. Design matrix of solvent and temperature combinations for response surface analysis 2-factor composite design for muscadine grape seed extracts…

grape seed extract at 65% ethanol / 35% water at 150°C …56 ix Chapter IV 1. Antimicrobial activity of muscadine grape seed extracts and phenolic standards against E.coli O157: H7, Listeria monocytogenes, and Salmonella typhimurium …………………………………………… x…

…of phenolic compounds by pressurized liquid extraction …………………... 48 2. Response surface plot for the total phenolic content of freeze-dried ethanol extract of Carlos muscadine grape seed using pressurized liquid extraction …………………………………………… 58 3…

…Response surface plot for the total phenolic content of freeze-dried ethyl acetate extract of Carlos muscadine grape seed using pressurized liquid extraction …………………………………………… 59 4. Response surface plot for the total phenolic content of oven-dried…

…ethanol extract of Carlos muscadine grape seed using pressurized liquid extraction …………………………………………… 60 xi 5. Response surface plot for the total phenolic content of oven-dried ethyl acetate extract of Carlos muscadine grape seed using pressurized…

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