| Author | Talungchit, Supitcha |
| Title | Enhancing resin-dentin bond effectiveness and durability: the role of ethanol-wet bonding technique, MMP-inhibition (chlorhexidine), and photoinitiator systems |
| URL | https://ir.uiowa.edu/cgi/viewcontent.cgi?article=3143&context=etd  https://ir.uiowa.edu/etd/2996 |
| Publication Date | 2012 |
| Degree | PhD |
| Discipline/Department | Oral Science |
| Degree Level | doctoral |
| University/Publisher | University of Iowa |
| Abstract | Current hydrophilic resin adhesives undergo hydrolytic degradation and show a decrease in bond strength over time. Nanoleakage and ultrastructure studies suggest that inadequately infiltrated collagen leads to enzymatic degradation and resin-dentin bond failure. Adequate degree of conversion (DC) of resin adhesives is also critical to resin-dentin bond strength and durability. The long-term goal of this dissertation is the realization of durable resin-dentin bond. It is hypothesized that ethanol-wet bonding technique (EW) may effectively facilitate the infiltration of hydrophobic monomers into hydrophilic acid-etched dentin by maintaining interfibrillar spacing, stiffening collagen matrix, and improving adhesive resin-demineralized dentin matrix miscibility. Chlorhexidine (CHX), Matrix Metalloproteinase-inhibitor (MMP-inhibitor), should further preserve collagen integrity and resin-dentin bond strength. Moreover, efficient photoinitiator systems that broaden light absorptivity and provide more reactive radicals may enhance polymerization. In this dissertation, a clinically-relevant EW protocol, 3×15s absolute ethanol rinsing, provided significantly higher microtensile bond strength (Ï€TBS) of a hydrophobic resin (70%BisGMA/30%TEGDMA) to dentin as compared to water-wet bonding (WW). All groups showed no significant drop of Ï€TBS after 1-year storage except EW without CHX application, showing marginally significant reduction in Ï€TBS (p=0.0558) suggesting MMP-inhibition by CHX in EW. These results were consistent with subsequent experiments. EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. Monomer molar concentration across the hybrid layer was significantly higher in EW than WW. An application of 2% CHX diacetate further preserved collagen banding in EW. WW showed more generalized spotted nanoleakage, while EW presented localized reticular nanoleakage. The use of Irgacure 819 (BAPO) alone and in combination with benzoyl peroxide (BPO) or camphorquinone (CQ) increased DC of hydrophobic and hydrophilic resins over resins containing the CQ/amine (4E) control. Only BAPO and BAPO/BPO demonstrated significantly higher immediate shear bond strength than CQ/4E. Within the limitations of these studies, EW improved resin-dentin bond durability by maintaining collagen interfibrillar spaces for efficient infiltration of a hydrophobic BisGMA/TEGDMA resin resulting in significantly higher Ï€TBS and monomer molar concentrations with less nanoleakage distribution within the hybrid layer than WW. CHX further maintained collagen integrity and Ï€TBS in EW. BAPO is a potential… |
| Subjects/Keywords | Chlorhexidine; Dentin adhesion; Ethanol-wet bonding; MMP-inhibition; Photoinitiator; Resin adhesives; Oral Biology and Oral Pathology |
| Contributors | Armstrong, Steven R. (supervisor) |
| Language | en |
| Country of Publication | us |
| Format | application/pdf |
| Record ID | oai:ir.uiowa.edu:etd-3143 |
| Repository | iowa |
| Date Retrieved | 2020-02-25 |
| Date Indexed | 2020-02-25 |
| Grantor | University of Iowa |
| Issued Date | 2012-05-01 07:00:00 |
| Note | <p>This thesis has been optimized for improved web viewing. If you require the original version, contact the University Archives at the University of Iowa: <a href="https://www.lib.uiowa.edu/sc/contact/">https://www.lib.uiowa.edu/sc/contact/</a>.</p> |
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…substrate and MMP inhibition at same day ...........................................................................108 Table 2-5 Weibull parameters by the type of combination of bonding substrate and MMP inhibition at 1 year…
…Chlorhexidine (CHX), Matrix Metalloproteinase-inhibitor (MMP-inhibitor), should further preserve collagen integrity and resin-dentin bond strength. Moreover, efficient photoinitiator systems that broaden light absorptivity and provide more…
…x29; to dentin as compared to water-wet bonding (WW). All groups showed no significant drop of μTBS after 1-year storage except EW without CHX application, showing marginally significant reduction in μTBS (p=0.0558) suggesting MMP…
…inhibition by CHX in EW. These results were consistent with subsequent experiments. EW maintained interfibrillar width and hybrid layer thickness for resin infiltration and retention. Monomer molar concentration across the hybrid layer was significantly…
…replacing water with ethanol within the acid-etched dentin, was introduced to solve this miscibility issue. 5 Chlorhexidine, a known MMP inhibitor, was introduced into the bonding process to inhibit proteolytic degradation within the hybrid layer…
