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Title Perineuronal nets and the inhibitory circuitry of the auditory midbrain: evidence for subtypes of GABAergic neurons
URL
Publication Date
Degree PhD
Discipline/Department College of Arts and Sciences / School of Biomedical Sciences
Degree Level doctoral
University/Publisher Kent State University
Abstract Perineuronal nets (PNs) are aggregates of extracellular matrix molecules that surround subsets of neurons throughout the central nervous system (Celio and Blumcke, 1994). PNs appear postnatally and often in association with the closing of periods of heightened plasticity, for example in zebra finch song nuclei around the time of song learning (Balmer et al., `09). PNs are present throughout the adult auditory system, however little information is available about subcortical PNs in general, and subcortical auditory PNs in particular (Sonntag et al., `15). Preliminary data indicates a large number of PNs in the inferior colliculus (IC), a major auditory hub. Reports of PNs in the inferior colliculus (IC) vary widely in the description of their distribution (Seeger et al., `94; Atoji et al., `97; Friauf, `00; Hilbig et al., `07), and although PNs are preferentially associated with GABAergic interneurons in other brain areas (Kosaka and Heizmann, `89; Hartig et al., `92), the neurotransmitter phenotype of IC PN-surrounded neurons has not been described. Additionally, nothing is known about the circuitry of PN-surrounded IC neurons. The likely association of PNs with GABAergic cells leads to the question of whether PN-surrounded IC cells fall into the small GABAergic (SG) or large GABAergic (LG) categories described by Ito et al. (2009). Preliminary data suggests an association with the LG group, based on an association between PNs and dense rings of terminals containing vesicular glutamate transporter 2 (VGLUT2- a marker of LG cells). There is currently no information about the projections of PN-surrounded IC neurons. As stated above, PNs are associated with interneurons in other areas, however all IC neurons are believed to have projections outside of the IC (Oliver et al., `91). An association with GABA cells makes it likely that PN-surrounded IC cells will project to either the medial geniculate body (MGB) or the contralateral IC, as these are the two main projections of IC GABA cells (Gonzalez-Hernandez et al., `96; Winer et al., `96). A larger proportion of the IC-MGB pathway is GABAergic, making this the more likely target. The lack of information about the distribution, neurotransmitter phenotype, and circuitry of PN-surrounded neurons in the IC is a major roadblock to understanding the function of PNs in hearing.
Subjects/Keywords Neurosciences; perineuronal nets; GABA; auditory; inferior colliculus; medial geniculate body; VGLUT2; hearing
Contributors Schofield, Brett (Advisor)
Language en
Rights unrestricted ; This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
Country of Publication us
Format application/pdf
Record ID oai:etd.ohiolink.edu:kent1468938365
Repository ohiolink
Date Retrieved
Date Indexed 2018-02-20
Grantor Kent State University

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…goal of ongoing research. Perineuronal nets (PNs) are aggregates of extracellular matrix molecules that surround subsets of neurons throughout the central nervous system; PN-surrounded neurons are often 1 inhibitory (Kosaka and…

…the understanding of subcortical PNs. The purpose of this dissertation is to provide a more complete understanding of the inhibitory circuitry of the IC by studying IC PNs. Perineuronal nets in the nervous system First described by Camillo Golgi in…

…provides evidence that PNs hold a key to distinguishing additional subtypes of IC GABAergic neurons. Perineuronal nets in the inferior colliculus Descriptions of PNs in the IC are few in number and vary widely regarding PN distribution among IC subdivisions…

…examine the roles of inhibition in development, aging, and pathological conditions of the auditory system. 12 CHAPTER II PERINEURONAL NETS AND GABAERGIC CELLS IN THE INFERIOR COLLICULUS OF GUINEA PIGS1 Perineuronal nets (PNs) are…

…Mellott, J.G., and B.R. Schofield (2014) Perineuronal nets and GABAergic cells in the inferior colliculus of guinea pigs. Front Neuroanat 7:53. 13 PNs have been demonstrated in the auditory system at the level of the cochlear nucleus…

…geniculate body NeuN neuronal nuclear antigen viii NLL nuclei of the lateral lemniscus PAG periaqueductal gray PB phosphate buffer PBS phosphate-buffered saline PN perineuronal net RB red RetroBeads SEM standard error of the mean SC…

…microscope (Zeiss). Individual cells, nets, and VGLUT2 rings were plotted within the IC. Cells that were associated with 2 or more labels were plotted with different symbols that denoted the specific combination of markers. Numerical 17…

…multiplying by section thickness to obtain the volume in which the stained nets were counted (section thickness was estimated as the thickness setting on the microtome during sectioning). The values across all three axes were not corrected for tissue…

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