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Title Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway
URL
Publication Date
Discipline/Department School of Chemistry and Molecular Biosciences
University/Publisher University of Queensland
Subjects/Keywords DENV-2; Dengue fever; Flavivirus; Virus Fusion; Structural Proteins; Envelope Proteins; 030406 Proteins and Peptides; 0601 Biochemistry and Cell Biology; 060108 Protein Trafficking
Language en
Country of Publication au
Record ID oai:espace.library.uq.edu.au:UQ:363952
Repository uqld1
Date Retrieved
Date Indexed 2020-08-18

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…expression and prME heterodimerisation. Immunofluorescence will be used to view protein expression, co-localisation of prM and E and cell surface trafficking. Fluorescent activated cell sorting and flow cytometry will be used to determine cell surface…

…2.6.4. MTT assay 2.7. Protein Expression 2.7.1. Cell lysate preparation 2.7.2. SDS polyacrylamide gel electrophoresis 2.7.3. Western transfer and immunoblotting 2.7.4. Immunofluorescence: Antibodies and Molecular Probes 8 2.7.5. Immunofluorescence…

…detection of prM and E 5.3. Results: Detection of E protein production with SDS-PAGE and Western immunoblot 5.4. Results: Detection of in-cell and surface expressed prME protein production with confocal microscopy 5.1.1. Analysis of cellular and surface…

…expressed prME protein production in transfected cells 5.1.2. Analysis of cellular and surface expressed prME in infected cells 5.5. Results: Analysis of the secretory pathway of prME 5.1.1. Tracking of the secretory pathway with molecular probes and…

…antibodies 5.1.2. Tracking of the secretory pathway with molecular probes and antibodies in infected cells 5.1.3. Localisation of E in infected cells 5.6. Discussion 5.1.1. Conclusions of cellular and surface expressed MPER E2 mutant protein 9 CHAPTER 6…

…versus immature prME protein 19 Figure 5. Immature to mature virion cryo-EM reconstruction 20 Figure 6. pH changes and E protein conformation 20 Figure 7. Dengue E protein showing Domains I, II, and III 21 Figure 8. Known ribbon diagrams of Dengue…

…III of Dengue E protein Dulbecco’s Modification of Eagles Medium Dimethyl Sulfoxide Deoxyribonucleic Acid Dengue Shock Syndrome Envelope glycoprotein Ethylene Diamine Tetra-acetic Acid Green Fluorescent Protein Endoplasmic Reticulum Fluorescent…

…Activated Cell Sorting Fetal Calf Serum Fusion From Within Fusion from Without Gravity Glycosaminoglycan Green Fluorescent Protein Hepatitis C Virus Human Embryonic Kidney cell line Human Immunodeficiency Virus Heparan Sulphate Immunofluorescence Internal…

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