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Author
Title Kisspeptin signaling and its role in canine reproduction: a basic understanding
URL
Publication Date
Degree Level masters
University/Publisher Universiteit Utrecht
Abstract There are several central and peripheral mechanisms that influence the GnRH secretion. For a long time it has been a mystery how these mechanisms influence the GnRH neurons. In recent years there have been exciting new discoveries in this field, especially with the discovery of kisspeptin and its receptor. Kisspeptin is a neuropeptide that seems to have an important role in the regulation of GnRH neurons in the hypothalamus in various mammalian species. It is, among other things, supposed to be involved in the onset of puberty and in the mediation of the positive feedback exhibited by estrogens on the GnRH neurons in females, which leads to the GnRH/LH surge. Although a lot of research has already been done, to our best knowledge, kisspeptin and its receptor have not yet been researched in the dog. Because the kisspeptin signaling has been called a major gatekeeper of the gonadotropic axis, it would be interesting to discover its role in the canine reproduction. To lessen the amount of dogs that are yearly euthanized all over the world because of overpopulation, it is important to find a non-surgical method of contraception that prevents estrous cyclicity. The kisspeptin signaling pathway could be a suitable target for developing an effective, safe, long-term or permanent non-surgical contraceptive for the bitch. The aims of the present study were to identify the canine KISS1 and KISS1R genes and to characterize the amino acid sequences of canine kisspeptin and the kisspeptin receptor. The identification and characterization were done in silico. The results show that the KiSS1 and the KiSS1R gene are present at the canine genome and a large homology is found between the canine peptides and the peptides found in other mammalian species. This gives a basis to believe that kisspeptin signaling is also important in GnRH secretion in the dog and that it may indeed be a suitable target for a non-surgical contraceptive.
Subjects/Keywords Canine, dog, bitch, kisspeptin, reproduction
Contributors Albers-Wolthers, C.H.J.
Language en
Rights info:eu-repo/semantics/OpenAccess
Country of Publication nl
Format text/plain
Record ID oai:dspace.library.uu.nl:1874/289517
Repository utrecht
Date Indexed 2016-09-27

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…step was done. Per 7.5 Ál of PCR product, 3 Ál of sap/exo mix KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING 7 (containing 2.5 Ál of shrimp alkaline phosphatase and 0.5 Ál of 2 units/Ál exonuclease I) was…

…heating cycles of 96░ C for 30 seconds, 50░ C for 15 seconds and 60░ C for 30 seconds. The second step was also done at 55░ C. KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING Chapter: Material and Methods The tercycle…

REPRODUCTION: A BASIC UNDERSTANDING 9 RESULTS IN SILICO IDENTIFICATION OF THE CANINE KISS1 AND KISS1R GENES KiSS1 Significant similarities with the cDNA sequence of human preprokisspeptin (accession NM_002256) were found on chromosome 38 of the…

…instead of an RF at its C-terminus, which is the same in mouse and rat kisspeptin 10, and the fifth amino acid is a valine instead of a serine (Fig. 2). KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING 10 Fig. 2…

…five exons and four introns. The red striped line shows the part where the gap was situated in the dog reference genome build 2.1 of the NCBI database. KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING Chapter: Results…

…primer combinations were used on all five KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING Chapter: Results Fig. 4 12 tissues using temperatures ranging from 55 to 65░ C. The tissues were chosen for their known…

…gelelectrophoresis. Lane 7 and 14 show a 100 bp size standard. Chapter: Results Fig. 5 KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING 13 DISCUSSION This study shows that both KiSS1 and KiSS1R are present on the canine genome. In…

…in the lower trace file. KISSPEPTIN SIGNALING AND ITS ROLE IN CANINE REPRODUCTION: A BASIC UNDERSTANDING Chapter: Discussion The gap in the KiSS1R gene was not completely sequenced. The trace files directly surrounding the gap were not of…

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