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Title Rapid Inline Derivatization of Primary and Secondary Amine Containing Drugs by Capillary Electrophoresis with Laser-Induced Fluorescence
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Publication Date
Degree PhD
Discipline/Department Chemistry
Degree Level doctoral
University/Publisher Florida International University
Abstract Despite the ongoing “war on drugs” the seizure rates for phenethylamines and their analogues have been steadily increasing over the years. The illicit manufacture of these compounds has become big business all over the world making it all the more attractive to the inexperienced “cook”. However, as a result, the samples produced are more susceptible to contamination with reactionary byproducts and leftover reagents. These impurities are useful in the analysis of seized drugs as their identities can help to determine the synthetic pathway used to make these drugs and thus, the provenance of these analytes. In the present work two fluorescent dyes, 4-fluoro-7-nitrobenzofurazan and 5-(4,6-dichlorotriazinyl)aminofluorescein, were used to label several phenethylamine analogues for electrophoretic separation with laser-induced fluorescence detection. The large scale to which law enforcement is encountering these compounds has the potential to create a tremendous backlog. In order to combat this, a rapid, sensitive method capable of full automation is required. Through the utilization of the inline derivatization method developed whereby analytes are labeled within the capillary efficiently in a minimum span of time, this can be achieved. The derivatization and separation parameters were optimized on the basis of a variety of experimentally determined factors in order to give highly resolved peaks in the fluorescence spectrum with limits of detection in the low µg/mL range.
Subjects/Keywords capillary electrophoresis; phenethylamines; fluorescence detection; derivatization; amphetamines; Analytical Chemistry
Contributors Bruce R. McCord; José R. Almirall; Yong Cai; David C. Chatfield; Deodutta Roy
Country of Publication us
Record ID oai:digitalcommons.fiu.edu:etd-2119
Repository fiu
Date Retrieved
Date Indexed 2020-01-06
Created Date 2013-11-14 08:00:00

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McCord*, Florida International University Correspondence: Dr. Bruce R. McCord, Department of Chemistry & Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL 33199 Email: [email protected] Fax: 305-348-3772 Keywords: amphetamines…

…17 neutral molecules to be separated. This is done by the formation of micelles around the analytes of interest, altering their electrophoretic mobility. 18 1.4 Electrophoretic Analysis of Phenethylamines Britt E. Turnquest, Bruce R

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