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Title Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing
Publication Date
Degree PhD
Discipline/Department Biological Sciences
Degree Level doctoral
University/Publisher NSYSU
Abstract Diabetes mellitus is characterized by elevated plasma glucose and increased rate of skin infection. Altered immune responses have been suggested to contribute to this prevalent complication. High glucose treatment reduced human β-defensin-3 (hBD3) expression of cultured keratinocytes. This pathogenic process involved inhibition of p38MAPK signaling, an event that resulted from increased formation of advanced glycation end product and showed worse anti-Staphylococcus aureus activity. In addition, high-glucose cultivated keratinocytes expressed reduced levels of human β-defensin-2 (hBD2) and pSTAT-1. Besides the impact on keratinocyte cultured under high glucose, the suboptimal interaction between keratinocytes and inflammatory cells also contributed to impaired diabetic wound healing. High-glucose environment enhanced interleukin (IL)-8 production via EGFR-ERK pathway in a ROS-dependent manner in keratinocytes. Treating diabetic rats with neutrophil inhibitor improved the healing. On the other hands, high glucose cultivated monocytes have significantly reduced production of IL-22, a molecule that is responsible for promoting keratinocyte migration. In summary, high glucose environment impared keratinocyte function directly or indirectly that contributed to impaired diabetic wound healing, and focusing on these defects will present a therapeutic approach to promote diabetic healing.
Subjects/Keywords hBD3; interleukin-8; keratinocyte; hBD2; high glucose; interleukin-22
Contributors Ching-Shuang Wu (chair); Lan, Cheng-Che (chair); Liu, Jong-Kang (chair); Chao, David (committee_member); Chen, Gwo-Shing (chair)
Language zh
Rights unrestricted ; Copyright information available at source archive
Country of Publication tw
Format application/pdf
Record ID oai:NSYSU:etd-0823115-001730
Repository nsysu
Date Indexed 2017-05-12
Grantor NSYSU

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…oxidative stress and increased interleukin-8 secretion from keratinocytes: New insights into impaired diabetic wound healing. Diabetes 2013; 62(7): 2530-8. Huang SM, Wu CS, Chao David, Wu JH, Li CC, Chen GS, et al. High-glucose cultivated…