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Title Synthesis of Novel Inhibitors of IdeS, a Bacterial Cysteine Protease Including Studies of Stereoselective Reductive Aminations
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University/Publisher University of Gothenburg / Göteborgs Universitet
Abstract Abstract The cysteine protease IdeS is an IgG degrading enzyme secreted by the bacterium Streptococcus pyogenes to evade the human immune system. In this thesis several inhibitors of IdeS have been synthesized and evaluated. Such inhibitors should be highly useful when elucidating the detailed mechanism of IdeS action. They might also have a potential as treatment of acute and severe infections caused by the bacteria. Further, IdeS has a therapeutic application of its own due to the proteolytic ability and an IdeS inhibitor might contribute during the development. Only irreversible, unselective inhibitors of IdeS were known five years ago. In this thesis, three strategies with the aim to synthesize and identify more inhibitors have been undertaken. Focus was first set on compounds with a substructure resembling the known inhibitors but with reversible warheads, i.e. nitrile, azide and aldehyde functions. The aldehyde derivatives were found to provide the first reversible inhibitors of IdeS. Then, to avoid covalent interactions and obtain more selective inhibitors, a substrate based strategy was undertaken. A 3-aminopiperidine fragment was used as replacement of either of the two residues adjacent to the scissile bond in IgG. Such fragments can be synthesized from N-protected 3-aminopiperidone and amino acid esters in reductive aminations in which a stereogenic center is formed. A series of di-, tri- and tetrapeptide analogues, together with eight peptides covering the cleavage site of IgG, were screened for their capacity to inhibit the cysteine proteases IdeS, SpeB and papain. Several analogues showed inhibition capacity, two compounds showed also high selectivity for IdeS. In contrast, none of the tested peptides showed any inhibition. Computational docking studies indicate that the identified IdeS peptide analogues and the non-active peptides do not share the same binding site in IdeS. Probably, the piperidine moiety hinders the inhibitor to enter the catalytic site. A more detailed study of the stereoselectivity in the reductive aminations affording the 3-aminopiperidine fragment showed that a large protecting group (trityl) together with a large reducing agent (NaBH(O-2- ethylhexanoyl)3) gave the highest diastereomeric ratio. The highest ratio obtained was 21:79 when Lproline methyl ester was used. The newly formed stereogenic center had the R-configuration, determined by chemical correlation. Computer based conformational analysis combined with Boltzmann distribution calculations implies an axial attack by the reducing reagent on the intermediary imine. To improve the potency of the two identified di- and tripeptide analogues synthetic routes to conformationally restricted N-containing bicyclic derivatives was undertaken in a third strategy. Five compounds with different bicyclic scaffolds were screened for their inhibition capacity towards IdeS and papain. One of the compounds was able to inhibit the first step of proteolytic cleavage of IgG by IdeS, a process usually completed in seconds.
Subjects/Keywords Cysteine protease inhibition,; IdeS, SpeB, Papain,; Conformational restriction; Peptidomimetics
Language en
Country of Publication se
Record ID handle:2077/26663
Other Identifiers 978-91-628-8347-8
Repository goteborg
Date Retrieved
Date Indexed 2018-01-11

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…5 1.3 THREE MEMBERS OF THE CA CLAN……………………………………… 9 1.3.1 papain……………….…………………………………………………….… 9 1.3.2 SpeB………………….………………………………………………….…… 9 1.3.3 IdeS………….……….………………………………………………….…… 10 1.4 THE BACTERIA/HOST-SYSTEM…………………………………..………. 11 2…

IdeS INHIBITORS………………………… 50 5.3 PEPTIDES AND PIPERIDINE ANALOGUES AS INHIBITORS………..………. 52 5.3.1 IdeS…………………….………………….……………..………… 52 5.3.2 papain/SpeB……………………………….……………..………… 55 5.3.3. pKa measurements………………………….……………..……… .. 56 5.3.4 In…

…thesis, three enzymes from the CA clan have been investigated; IdeS (IgG degrading enzyme from Streptcococcus pyogenes) (C66) and SpeB (Streptococcal pyogenic exotoxin B) (C10), both from the same bacterium, and…

…also the plant cysteine protease papain (C1) (FIGURE 1). IdeS papain SpeB FIGURE 1. IdeS2, SpeB3 and papain4 adopt a so-called papain-like fold with the catalytic site located between the two domains shown as Richardson diagrams…

…showed inhibitory activity (FIGURE 9D). 8 FIGURE 9A-D. Recently published non-covalent and nonpeptide-like cysteine protease inhibitors. 1.3 THREE MEMBERS OF THE CA CLAN Papain, SpeB and IdeS are cysteine proteases catalyzing the…

…hydrolysis of the same substrate, the human antibody immunoglobulin G (IgG). In this thesis the main focus is set on IdeS, whereas SpeB and papain have been used for selectivity studies. 1.3.1 Papain is a plant cysteine protease found in papaya…

…present study. FIGURE 10. 3D structures of SpeB6 A) the C47S-mutated structure (PDB ID: 2JTC); B) the E-64 inhibited structure (PDB ID: 2UZJ). 1.3.3 IdeS (Immunoglobulin G-degrading enzyme of S. pyogenes, also called…

…Mac-1) was discovered by two independent research teams in 2001 and 2002.40-42 As the name indicates the cysteine protease catalyzes the hydrolysis of the human antibody IgG.43 IdeS is secreted by S. pyogenes as a mature enzyme of 339 residues and…

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