Full Record

New Search | Similar Records

Author
Title Evaluation of Peanut Skin Extract, Grape Seed Extract, and Grape Seed Extract Fractions to Reduce Populations of Select Foodborne Pathogens
URL
Publication Date
Date Accessioned
Degree MSin Life Sciences
Discipline/Department Food Science and Technology
Degree Level masters
University/Publisher Virginia Tech
Abstract Grape seed extract (GSE) and peanut skin extract (PSE) are waste products in the wine and peanut industries. Both extracts have high concentrations of polyphenols, known to possess antioxidant and antimicrobial properties. A subcategory of polyphenol is procyanidin, which can be divided into two types, type A and type B. Type A (PSE), contains two single bonds connecting the phenolic groups while type B (GSE), contains one single bond connecting the phenolic groups. The minimum inhibitory concentration (MIC) of the two extracts was evaluated for their antimicrobial effect on Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella Typhimurium using the pour plate method. GSE was found to have a significantly lower MIC (p ≤ 0.05) than PSE for L. monocytogenes (GSE=60.60ppm, PSE=not found), S. aureus (GSE=38.63ppm, PSE=51.36ppm), and S. Typhimurium (GSE=45.73ppm, PSE=60.60ppm). There was no significant difference in inhibition of E. coli O157:H7 (GSE=47.44ppm, PSE=51.13ppm). Since GSE, contributed to greater pathogen inhibition, its extract was fractionated into monomer and oligomers components. Growth curves of all four pathogens inoculated in the monomer and oligomer fractions were compared using the BioScreen method. Oligomers inhibited growth of L. monocytogenes, S. aureus, and E. coli O157:H7 while monomers inhibited growth of S. Typhimurium. These results indicate that an extract with type B procyanidins that are high in oligomers may be more effective as antimicrobials. Type B procyanidins have also been shown to prevent bacterial adhesion, as is the case with urinary tract infections, and may aid in the prevention of biofilms.
Subjects/Keywords Polyphenols; natural antimicrobials; grape seed extract; Vitis vinifera; peanut skin extract; E. coli O157:H7; Listeria monocytogenes; S. aureus; Salmonella Typhimurium
Contributors Boyer, Renee R. (committeechair); O'Keefe, Sean F. (committee member); Neilson, Andrew P. (committee member); Williams, Robert C. (committee member)
Rights In Copyright
http://rightsstatements.org/vocab/InC/1.0/
Country of Publication us
Record ID handle:10919/48896
Repository vt
Date Retrieved
Date Indexed 2020-12-04
Grantor Virginia Polytechnic Institute and State University
Issued Date 2014-06-10 00:00:00
Note [degree] Master of Science in Life Sciences;

Sample Images | Cited Works

.